Method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases

A technology for post-harvest fruit diseases and bamboo leaf flavonoids, which is applied in the directions of yeast-containing food ingredients, fruit and vegetable preservation, food ingredients as antimicrobial preservation, etc., can solve the pressure of large economic costs, the control effect cannot meet the requirements, and the way of use To achieve the effect of reducing the decay rate, low cost, and avoiding harm to people without waiting for the problem

Active Publication Date: 2016-07-27
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that most of the antagonistic bacteria reported in the literature are used to prevent and treat pathogenic bacteria in the wounds of fruits and vegetables, rather than directly used on the skin of fruits and vegetables to prevent the invasion of pathogenic bacteria in normal fruits and vegetables; on the other hand, under commercial production conditions, due to the uncertainty of environmental conditions, Its control effect either fails to meet the requirements or is unstable, and the control cost is higher compared with chemical fungicides; antagonistic bacteria are used as preservatives, and their use is not as convenient as chemical fungicides
In addition, the characteristic that antagonistic yeast needs to be effective at a higher concentration will also generate great economic cost pressure

Method used

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  • Method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases
  • Method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases
  • Method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Pichia carribbica (P.carribbica) was screened by our laboratory, applied for a Chinese invention patent and authorized, patent number ZL201010198119.6, patent name Pichia carribbica, in fruit storage and preservation application and method of use. NYDA (2% agar (mass meter) added on the basis of NYDB medium) medium was stored at a low temperature of 4°C. Yeast extract 5g in NYDB medium, beef extract 8g, glucose 10g, purified water 1000ml, natural pH.

[0026] The culture procedure is as follows: (1) solid activation: inoculate Pichia calibike in NYDA medium, and culture at 28°C for 48 hours; (2) liquid culture: put 50ml of NYDB seeds in a 250ml Erlenmeyer flask for culture Base, use an inoculation loop to insert a loop of activated Pichia calibikis, and culture it at 180rpm and 28°C for 20h; (3) Centrifuge and resuspend: centrifuge the above yeast culture mixture at 8000g for 10min, And washed twice with sterile distilled water to remove the culture medium, ...

Embodiment 2

[0027] Example 2 Inhibitory effect of bamboo leaf flavonoids combined with Pichia calebicus on apple postharvest blue mold

[0028] 1) Test plan

[0029] The fruit for testing has been carefully selected, and the fruit should be selected as uniform as possible, without scratches on the surface! Mechanical injury and pest injury. The apples were punched with a sterile punch, and the surface wound was 5mm (diameter) × 3mm (depth). Add 30 μl of the following treatment solution equally to each wound: (1) 1×10 8 cells / mL of Pichia calibicus suspension (containing bamboo leaf flavonoids of various concentrations); (2) 1×10 8 cells / mL of Pichia calibicus suspension; (3) sterile water. After 3 hours, inoculate 30 μL of pathogenic bacteria spore suspension to the fruit wound respectively: inoculate 5×10 4 spores / mL of Penicillium expanusm spore suspension. After drying naturally, put the fruit into a plastic basket and seal it with a plastic wrap, and cultivate it in a constant t...

Embodiment 3

[0035] Example 3 Effects of Bamboo Leaf Flavonoids Combined with Pichia Calibicus on Natural Rot and Storage Quality of Apples

[0036] 1) Test plan

[0037] Spray the following treatment solution evenly on the surface of the fruit and let it dry naturally: (1) 1×10 8 cells / mL of Pichia calibicus suspension (BLF concentration is 100 μg / mL); (2) 1×10 8 cells / mL Pichia calibicus suspension; (3) BLF (100 μg / mL concentration) and (4) sterile water. Then, put the processed fruit into a plastic basket and seal it with plastic wrap. Store in a constant temperature incubator at 20°C (RH95%) for 35 days. From the 10th day, the fruit was taken out every 5 days to count the number of rotten fruits, and the rotten situation was observed. After 35 days, the storage quality of the fruit was measured. Each treatment was repeated 3 times, and the whole experiment was repeated 2 times. Inhibitory effect of bamboo leaf flavonoids combined with Pichia calibbica on fruit natural decay expre...

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Abstract

The present invention discloses a method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases, and belongs to the biological prevention and treatment field of fruit postharvest diseases. The pichia caribbica is activated, the activated pichia caribbica is inoculated into a NYDB culture medium to be cultured for 20 h, the cultured pichia caribbica is centrifuged to obtain the pichia caribbica cells, the pichia caribbica cells are diluted into pichia caribbica suspension at 1x10^8 cells/mL using the bamboo leaf flavonoid at twice final concentration (the final concentration of the bamboo leaf flavonoid is 100 [mu]g/mL-500 [mu]g/mL); the pichia caribbica suspension is uniformly sprayed on apples and the apples are dried naturally; the dried apples are placed in plastic baskets, the appless are sealed with preservation films, and the apples are placed in a room temperature (20 DEG C) to be stored. The combination of the bamboo leaf flavonoid and the pichia caribbica improves the effectiveness of the biological prevention and treatment of the apple post-harvest diseases. The method is easy to use, convenient to operate, good in effects and low in costs.

Description

technical field [0001] The invention relates to a method for controlling postharvest diseases of apples by combining bamboo leaf flavonoids (BLF) with Pichiacaribbica, and belongs to the field of biological control of postharvest diseases of fruits. Background technique [0002] Apple is one of the four major fruits in the world. It is the most important fruit tree species cultivated in temperate regions of the world and occupies an important position in the world fruit market. Apples are very rich in nutrients. Contains protein, fat, carbohydrates, vitamin C, vitamin B1, vitamin B2, carotene, calcium, phosphorus, iron, citric acid, tartaric acid and other organic acids, as well as pectin and cellulose. At present, there are about 80 apple producing countries (regions) in the world, and China and South America are the countries and regions with the fastest growing apples in the past 20 years. In 2001, the world's apple area was 5.685 million hectares, and the output was 6....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A23B7/155
CPCA23B7/154A23B7/155A23V2002/00A23V2200/10A23V2250/2116A23V2250/76
Inventor 张红印任晓锋李万海张晓云杨其亚陈亮亮朱淑云
Owner JIANGSU UNIV
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