Application of arginine in the preparation of preservatives for improving the ability of Prucheminic acid production and biocontrol efficacy of Metchiella citrinum

A technology that can generate arginine and is used in food ingredients as antimicrobial preservation, application, and fruit and vegetable preservation. It can solve the problems of large difference in effect, high production cost, and short shelf life, and achieve the goal of solving agricultural residues. and environmental pollution problems, good application potential, effect of enhancing potency

Active Publication Date: 2022-05-20
SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the traditional general-purpose chemical fungicides, there is still a certain gap in the effect of these commercial biocontrol agents and the antagonistic yeast with potential commercial value reported by research.
The main reasons are: the effect of antagonistic yeasts in the actual application process is quite different from that in the laboratory research; the stability is not good due to the influence of factors such as the concentration of pathogenic bacteria, the physiological state of the fruit, and the storage environment; the shelf life is short, and it is difficult to develop long-term effective formulations ; high production costs, etc.
These problems lead to slow progress in the large-scale commercial application of antagonistic yeast

Method used

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  • Application of arginine in the preparation of preservatives for improving the ability of Prucheminic acid production and biocontrol efficacy of Metchiella citrinum
  • Application of arginine in the preparation of preservatives for improving the ability of Prucheminic acid production and biocontrol efficacy of Metchiella citrinum
  • Application of arginine in the preparation of preservatives for improving the ability of Prucheminic acid production and biocontrol efficacy of Metchiella citrinum

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Embodiment Construction

[0024] In order to make the object, technical solution and beneficial effect of the present invention clearer, preferred embodiments of the present invention will be described in detail below.

[0025] 1. Experimental method

[0026] 1. Effects of different amino acids on the production of M.citriensis pucherin

[0027] With different concentrations of amino acids (0, 1, 5, 10, 100mmol·L -1 ) PDA plate to test the effect of amino acids on the production of pucherin by M.citriensis. Fixed content of 35mg·L -1 FeCl 3 The volume of PDA medium on each plate is 10 mL. After the medium is fully solidified, take 20 μL of 1×10 8 cells mL -1 The M.citriensis bacterial suspension was evenly spread on the central axis of the PDA plate, and the spreading range was 60mm×2.6mm. After the PDA plate was cultured at 28°C for 3 days, the width of the pucemin pigment circle was measured. A total of 20 amino acids were tested: alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), ...

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Abstract

The invention discloses the application of arginine in the preparation of a preservative for improving the production ability of Placemin acid by Metchi citriens, and a method for improving the production ability of Placemin acid by using arginine. The application of arginine in the preparation of preservatives for improving the biological control effect of Metchi citrus yeast on post-harvest fruit diseases, and the method of using arginine to improve the biological control effect of Metchi citrus yeast on post-harvest fruit diseases, the effect is clear, Significantly, it can effectively solve the problems of agricultural residues and environmental pollution caused by the use of pesticides, has good application potential in the biological control of postharvest diseases of fruits, and also provides an excellent method for developing microbial pesticides and enhancing their efficacy.

Description

technical field [0001] The invention belongs to the technical field of post-harvest disease biological control of fruits, and relates to the application of arginine in the preparation of a preservative for improving the ability of Metschnikowia citriensis to produce clementine acid and its biocontrol efficacy. Background technique [0002] Since Wilson and Pusey reviewed the application potential of using antagonistic bacteria to carry out postharvest biological control of postharvest fruits in 1985, the research reports on antagonistic yeasts have shown exponential growth, and the research on using antagonistic yeasts to control postharvest fruit diseases has also made great progress. progress, some antagonistic yeasts with good comprehensive properties have also achieved commercial applications, such as Candida oleophila (Aspire TM , Ecogen, Langhorne, PA, US), Cryptococcus albidus (YieldPlus TM , Lallemand, Montreal, Canada), Candida sake (Candifruit TM , IRTA, Lleida, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B7/155C12N1/38C12R1/645
CPCA23B7/155C12N1/38A23V2002/00A23V2200/10Y02A40/90
Inventor 曾凯芳王淑培易兰花邓丽莉姚世响阮长晴
Owner SOUTHWEST UNIV
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