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Magnetic nano immobilized enzyme catalysis production method of soya isoflavone

A technology of isoflavone aglycones and soybean isoflavones, which is applied in the production of bulk chemicals, immobilization on or in inorganic carriers, fermentation, etc., can solve the problems of magnetic nano-immobilized enzymes that have no relevant patents and reports, and achieve Easy separation, high glycoside extraction rate, simple process and easy operation

Inactive Publication Date: 2009-09-16
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the application of magnetic nano-immobilized enzymes in catalyzing the conversion of glycoside-type soybean isoflavones into soybean isoflavone aglycones has not yet seen relevant patents and reports

Method used

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  • Magnetic nano immobilized enzyme catalysis production method of soya isoflavone
  • Magnetic nano immobilized enzyme catalysis production method of soya isoflavone
  • Magnetic nano immobilized enzyme catalysis production method of soya isoflavone

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The method for producing soybean isoflavone aglycone by magnetic nano-immobilized enzyme conversion glycoside soybean isoflavone raw material comprises the following steps:

[0038] Step 1: Enrichment of Glycosidic Soybean Isoflavones by Supercritical Fluid Extraction

[0039] Take 100g of defatted soybean meal, fully wet it with 100mL of 95% ethanol entrainer, and put it into the extraction kettle of a supercritical fluid extraction device. At an extraction pressure of 30MPa, an extraction temperature of 50°C, a carbon dioxide flow rate of 6L / h, and 200mL of 95% ethanol entrainer Under the conditions, static extraction was performed for 120 minutes first, followed by dynamic extraction for 60 minutes. Release the extract from the separation tank, and evaporate under reduced pressure to separate the ethanol entrainer from the extract to obtain the entrainer and the extract. The obtained entrainer can be reused, and the extract is glycoside-based soybean isoflavones afte...

Embodiment 2

[0051] Soybean germ powder was used as the raw material for supercritical fluid extraction and enrichment of glycoside-type soybean isoflavones, ferric iron tetroxide magnetic nanoparticles were used, and the others were the same as in Example 1.

[0052] That is, 1.1 mg of high-purity soybean isoflavone aglycone finished product was obtained.

Embodiment 3

[0054] Defatted soybean powder was used as the raw material for supercritical fluid extraction and enrichment of glycoside-type soybean isoflavones, and titanium oxide magnetic nanoparticles were used. Others were the same as in Example 1.

[0055] That is, 0.9 mg of high-purity soybean isoflavone aglycone finished product was obtained.

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Abstract

The invention relates to a magnetic nano immobilized enzyme catalysis production method of soya isoflavone, comprising the following steps: (1) concentrating glycoside type soya isoflavone raw material using supercritical fluid extraction; (2) extracting soya isoflavone glycoside hydrolase; (3) preparing the magnetic nano immobilized enzyme; (4) catalyzing the glycoside type soya isoflavone raw material using magnetic nano immobilized enzyme; (5) extracting soya isoflavone aglycone. The preparation method has features of simple and easily-operable supercritical extraction technology, innocuity and no-residue of carbon dioxide in operation process, high glycoside extraction ratio; simple preparation method of magnetic nano immobilized enzyme, simple recovery and repeat use of the enzyme using the external magnetic field; no destruction of the soya isoflavone aglycone and biological activity, high content of the soya isoflavone aglycone. The sum of the soya isoflavone aglycone concentration is 82.7% in the soya isoflavone aglycone product and the content of genistein is 90.3%.

Description

technical field [0001] The invention relates to a method for converting soybean isoflavone glycosides enriched by magnetic nano-immobilized enzyme biocatalysis supercritical fluid extraction into soybean isoflavone aglycone. Background technique [0002] Soybean isoflavones are a class of secondary metabolites formed during the growth of soybeans. There are 12 types in total, including 3 soybean isoflavone aglycones, genistein, daidzein, and glycitein. See figure 1 , and the composition of nine glycoside isoflavones (soybean isoflavone glycosides) formed by linking them with glucose, acetylglucose, and malonylglucose by β-glycosidic bonds, see figure 2 . Studies have proved that soybean isoflavone aglycone is the active form of soybean isoflavone to exert pharmacological functions. They are active aglycones, and genistein is the most active. However, the content of active aglycone is extremely low, only accounting for 2-3% of the total amount of soybean isoflavones. Soy ...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12N11/14
CPCY02P20/54
Inventor 罗建平潘利华查学强王贵娟徐学玲
Owner HEFEI UNIV OF TECH
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