33results about How to "Excellent electrochemical response" patented technology

The invention provides preparation of a glassy carbon electrode modified by gold nanoparticles wrapped with ionic liquid and detection on cholesterol by the glassy carbon electrode. The preparation method comprises the following steps: at first, performing in-situ reduction on chloroauric acid by using sodium borohydride in an ionic liquid two-phase system to obtain gold nanoparticles wrapped with the ionic liquid; then, directly fixing cholesterol oxidase (ChOx) on a gold nanoparticle composite material wrapped with the ionic liquid; and finally, dropwise coating the surface of the glassy carbon electrode with the compounded composite material to form a modified glassy carbon electrode. The modified glassy carbon electrode has good electrochemical response to cholesterol and can be used for directly detecting cholesterol. The detection on cholesterol by the prepared glassy carbon electrode has the characteristics of wide linear range and low detection limitation.

The invention provides a method for preparing a multi-target DNA (Deoxyribose Nucleic Acid) biosensor using metal ions as markers. According to the invention, the method that the metal ions are used as the markers to construct the DNA biosensor and metallothionein (MT) is used as a bridge to achieve connection of the metal ions and a DNA detection probe sequence is proposed for the first time. Firstly, Zn<2+>, Cd<2+> and Pb<2+> are respectively marked on detection probes of three virus DNA segments by the metallothionein, then the marked detection probes are crossbred with corresponding DNA capture probes fixed on a gold substrate and target DANs in samples to form a sandwich structure, and finally, the metal ions are dissociated by hydrochloric acid and are detected by an anodic-stripping voltammetric method, so that the purpose of detecting the target DNAs is achieved. The sensor prepared by the method can simultaneously detect various target DNAs and has the characteristics of high sensitivity and low limit of detection.

The divisional application discloses a flexible glucose electrochemical sensor, belonging to the technical field of electrochemical sensors. A flexible electrode substrate is polydimethylsiloxane (PDMS), and a dendritic structure nano-silver conducting layer is arranged on the PDMS substrate. The preparation method comprises the steps of carrying out self-assembly of indium tin oxide (ITO) glass,carrying out electrochemical deposition of a dendritic structure nano-silver film layer on the ITO glass, putting the ITO glass with the nano-silver conducting layer into a PDMS solution for curing, and then removing the PDMS film from the ITO glass to obtain the flexible glucose electrochemical sensor based on a dendritic nano-silver structure. The flexible glucose electrochemical sensor providedby the invention has a good response to glucose, is simple in preparation method and is easy to mass production, thus being expected to be widely applied to industrial and agricultural production aswell as life science research, especially wearable medical equipment field.

The invention discloses a preparation method of a biosensor and a modified electrode that take the substrate of attapulgite clay, the sensor takes the substrate of the attapulgite clay with a natural nano structure, utilizes the unique pore canal structure and the excellent adsorptive power of the attapulgite clay, fixes glucose oxidase (GOD) on the surface of the attapulgite clay, coats the attapulgite clay with adsorbed oxidase on the surface of a glassy carbon electrode, and drops and coats a layer of Nafion methanol solution on the surface, thus forming the Nafion-GOD / ATP / GC modified electrode. The modified electrode, a platinum sheet electrode, a saturated calomel electrode form an electrolytic cell, which is further connected with a converter and a display screen into the biosensor. The biosensor has quick response time, high sensitivity, simple and convenient production method, good repeatability and stability, and convenient mechanization, integration and commercialization.

The invention discloses a flexible glucose electrochemical transducer based on a dendritic nano silver structure, and belongs to the technical field of electrochemical transducers. The base of a flexible electrode is PDMS, and a nano silver conducting layer of a dendritic structure is arranged on the PDMS base. The preparing method comprises the steps of self-assembling ITO glass, electrochemically depositing a nano argentea conducting layer of the dendritic structure on the ITO glass, putting the ITO glass with the nano silver conducting layer into a PDMS solution to be solidified, and uncovering the PDMS film from the ITO glass after solidifying to obtain the flexible glucose electrochemical transducer based on the dendritic nano silver structure. The flexible glucose electrochemical transducer responds well to glucose, is simple in preparing method, quantification production is easy to carry out, and the transducer is expected to be widely applied to the field of industrial engineering, the field of agricultural production, the field of life science research, and particularly the field of wearable medical facilities.

The invention discloses a method for regenerating carbon fiber ultramicroelectrode. The method is characterized in that a reference electrode and a poisoning electrode are placed in deionized water, through a constant potential current time method, the reference electrode and the poisoning electrode are activated, wherein no chemical substance is added in the deionized water. The above method has good activation effect, compared with the poisoning electrode, the electrochemistry response of the processed carbon fiber ultramicroelectrode is obviously increased, and the electrochemistry performance of the regenerated electrode is stable.

The invention belongs to the technical field of electrochemical and nanometer materials, and relates to a DNA sensing electrode preparation method, wherein a DNA sensing electrode based on anionic porphyrin-carbon nano-tube modification is prepared by using the pi-pi interaction between anionic porphyrin and carbon nano-tubes. The modification steps comprise: ultrasonically dispersing carbon nano-tubes (CNTs) in a Tris-HCl buffer liquid, adding ssDNA and complementary paired ssDNA thereof (are hybridized to form dsDNA), adding an anionic porphyrin (TSPP) solution, and carrying out drop coatingto obtain the TSPP / dsDNA / CNTs / GCE modified electrode. According to the present invention, the preparation method has characteristics of simple process and short preparation time; the prepared electrode is the good detection electrode; and the prepared DNA biological sensing electrode combines the specificity of DNA complementary pairing and the high sensitivity of electrochemical detection, and is expected to provide the new way for the early diagnosis of cancer cells.

The invention discloses a metal phthalocyanine-MXene composite material, a preparation method and an application thereof. Wherein, the method for preparing metal phthalocyanine-MXene composite material comprises: (1) metal phthalocyanine is mixed with first solvent, obtains metal phthalocyanine solution; Said metal phthalocyanine solution is added into water, obtains metal phthalocyanine nanostructure (2) mixing the metal phthalocyanine nanostructure, MXene material and the second solvent to obtain the metal phthalocyanine-MXene composite material. The process of the method is simple and repeatable, and the materials used are easy to synthesize, low in price, and easy to be prepared on a large scale, which is conducive to realizing the commercialization of materials and devices. By adopting this method, metal phthalocyanine nanostructures can be introduced between MXene layers to act as interlayer spacers, thereby effectively preventing the restacking effect of MXene and increasing the electrochemically active sites on the surface of MXene, which is useful for the electrochemical redox process. There is also a significant enhancement of the ion mobility, which in turn can improve the electrochemical response to charge storage.

The invention relates to a preparation method of an optical composite nano-fiber material. The method comprises the steps of 1) preparing a nano gold-multi-wall carbon nano tube compound Au-MCNT; 2) preparing a spinning solution; 3) preparing the optical composite nano-fiber material in an electrostatic spinning way. In the electrostatic spinning, the prepared uniform and transparent precursor electrostatic spinning solution is arranged in an injector, a positive electrode of a high-voltage electrostatic generator is connected with a needle, a negative electrode is connected onto a clean bare electrode, the liquid is supplied by adopting a trace injection pump and directly applied to the injector needle through the high-voltage electrostatic power generated by the high-voltage electrostatic generator, and the optical composite nano-fiber is collected onto the bare electrode. The nano gold-multi-wall carbon nano tube compound with good conductivity and large specific surface area and capable of stably and firmly loading a great amount of trisruthenium Ru(bpy)3<2+> is doped with the spinnable macromolecular nylon 6 with good stability to obtain the precursor electrostatic spinning solution, and the optical composite nano fiber is obtained through the one-step electrostatic spinning method.

The invention relates to a graphene-single-wall carbon nano tube-nano cerium compound modified sensing electrode which comprises an electrode body and a modification article covering the electrode body, wherein the modification article comprises a compound of graphene, a single-wall carbon nano tube and nano cerium. The invention further relates to a preparation method and an application of the sensing electrode. An electrochemical sensor is assembled by replacing a conventional electrode with the graphene-single-wall carbon nano tube-nano cerium compound modified sensing electrode; and when metronidazole and ciprofloxacin in a solution is determined, two oxidation peaks with a very good separation degree can be obtained and have better peak shapes. Therefore, the preparation can be used for improving electrochemical determination accuracy.

The invention discloses a wearable cloth-based electrochemical sweat sensing device and method. The device includes a sweat collection layer, a sweat detection layer, a waste liquid collection layer and a fixed pressing layer. The sweat collection layer is used to quickly collect sweat on the skin surface. The sweat detection layer includes a folded cloth-based detection chip, the cloth-based detection chip includes a cloth-based carrier, an auxiliary unit and a sweat detection unit, the waste liquid collection layer includes a paper-based waste liquid collection sheet, and the fixed pressing layer is used to fix the cloth-based detection The chip and the paper-based waste liquid collection sheet are connected to the external circuit. Sweat penetrates into the hydrophilic area of ​​the cloth-based detection chip through the sweat collection layer, and then reaches the working electrode for detection. The paper-based waste liquid collection sheet absorbs the sweat overflowing from the working electrode, and the cloth-based detection chip transmits the detection signal to the external circuit for analysis. Finally, the sweat detection results are obtained, and the low-cost, non-invasive, stable real-time continuous monitoring of the sweat components on the human skin surface is realized, which is of great significance to the monitoring of human health status.

The invention is a solid-phase electrochemiluminescence detection method for aniline, and the specific method includes: 1) preparation of optical composite nanofiber Ru-AuNPs-PA6; 2) construction and optimization of a solid-phase electrochemiluminescence sensing system; 3 ) Quantitative detection of aniline. The electrochemiluminescence active material bipyridyl ruthenium-doped nano-gold colloid / nylon 6 optical composite nanofiber Ru‑AuNPs‑PA6 was prepared by electrospinning, and the solid-phase electrochemiluminescence sensing system was constructed by using Ru‑AuNPs‑PA6. Relevant parameters were optimized, and the constructed solid-phase electrochemiluminescence sensing system was used to quantitatively detect aniline. The invention establishes a solid-phase electrochemiluminescence detection method for aniline with the characteristics of high sensitivity, high stability, wide linear range, low detection limit, reusable sensing system and the like.

The invention discloses nickel metal organic framework nanomaterials. The preparation steps are as follows: mixing an ethylene glycol solution of a divalent nickel salt and a dimethylformamide solution of terephthalic acid, stirring evenly, and heating the The hydrothermal reaction is carried out to obtain the precipitate produced by the reaction; the precipitate is washed with dimethylformamide and ethanol, and then dried to obtain the nickel metal organic framework nanomaterial. The sensor electrode prepared by the invention has a wide detection range for glucose, can detect 0.5 μM-8.062 mM glucose, has high detection sensitivity, and has good anti-interference performance for ascorbic acid, uric acid, dopamine and sodium chloride.

The invention belongs to the technical field of electrochemical and nanometer materials, and relates to a DNA sensing electrode preparation method, wherein a DNA sensing electrode based on anionic porphyrin-carbon nano-tube modification is prepared by using the pi-pi interaction between anionic porphyrin and carbon nano-tubes. The modification steps comprise: ultrasonically dispersing carbon nano-tubes (CNTs) in a Tris-HCl buffer liquid, adding ssDNA and complementary paired ssDNA thereof (are hybridized to form dsDNA), adding an anionic porphyrin (TSPP) solution, and carrying out drop coatingto obtain the TSPP / dsDNA / CNTs / GCE modified electrode. According to the present invention, the preparation method has characteristics of simple process and short preparation time; the prepared electrode is the good detection electrode; and the prepared DNA biological sensing electrode combines the specificity of DNA complementary pairing and the high sensitivity of electrochemical detection, and is expected to provide the new way for the early diagnosis of cancer cells.

The invention discloses a method for regenerating carbon fiber ultramicroelectrode. The method is characterized in that a reference electrode and a poisoning electrode are placed in deionized water, through a constant potential current time method, the reference electrode and the poisoning electrode are activated, wherein no chemical substance is added in the deionized water. The above method has good activation effect, compared with the poisoning electrode, the electrochemistry response of the processed carbon fiber ultramicroelectrode is obviously increased, and the electrochemistry performance of the regenerated electrode is stable.

The invention relates to a graphene-single-wall carbon nano tube-nano cerium compound modified sensing electrode which comprises an electrode body and a modification article covering the electrode body, wherein the modification article comprises a compound of graphene, a single-wall carbon nano tube and nano cerium. The invention further relates to a preparation method and an application of the sensing electrode. An electrochemical sensor is assembled by replacing a conventional electrode with the graphene-single-wall carbon nano tube-nano cerium compound modified sensing electrode; and when metronidazole and ciprofloxacin in a solution is determined, two oxidation peaks with a very good separation degree can be obtained and have better peak shapes. Therefore, the preparation can be used for improving electrochemical determination accuracy.

The invention discloses a preparation method for a nanogold-nanofiber functional composite modified electrode. The preparation method specifically comprises the following steps of (1) preparing a spinning solution; (2) performing electrostatic spinning to prepare composite nanofibers to form a composite nanofiber PA6-MCWNTs modified electrode; and (3) performing functionalization on composite nanofibers through electro-deposition nanogold, namely immersing the composite nanofiber PA6-MCWNTs modified electrode into deposition liquid containing HAuCl4, reducing HAuCl4 into nanogold by adopting a multi-potential step change method, and synchronously and directly depositing the nanogold on the surfaces of PA6-MCWNTs composite nanofibers, thus obtaining the nanogold / nanofiber functional composite AuNPs-PA6-MCWNTs modified electrode. The preparation method can be used for obtaining a functional composite electrode modified material with the characteristics of high stability, large specific surface area, high biological compatibility, high electron transmission rate, uniform nano diameter aperture distribution and the like.

The invention discloses an electrochemical method for on-line analysis of nitro reduction reaction. The method comprises the following steps: (1) placing the mixed solution of the polyelectrolyte and the organic solvent in the dialysis membrane device; (2) placing the working electrode, the counter electrode and the reference electrode in the dialysis membrane device in step (1) After that, the dialysis membrane device is placed in the nitro compound reaction solution, and then connected to the electrochemical system circuit, the nitro reduction reaction can be analyzed and detected. The material used in the analytical method of the present invention has low toxicity, and can not only simplify the detection device for the nitro reduction reaction, but also can quickly complete the detection process through the diffusion of the nitro compound on the microelectrode, and because the membrane technology is applied to Electrochemical voltammetric detection technology, the combination of the two technologies, greatly solves the feasibility of online monitoring of organic reactions.

The invention relates to the technical field of the electrochemical analysis, and particularly relates to a preparation method of a carboxylated multiwalled carbon nanotube modified electrode based onITO and a method for measuring uric acid by using the electrode. An electrochemical measuring system is composed of a self-made electrode serving as a working electrode, an Ag / AgCl electrode servingas a reference electrode, a platinum wire serving as an auxiliary electrode, and an electrochemical workstation. The preparation method comprises the following steps of: taking ITO glass as a substrate, alternately self-assembling PDDA and PSS on the ITO surface to form a film, and uniformly coating a layer of carboxylated multiwalled carbon nanotube on the self-assembled layer. The invention aimsto apply the carboxylated multiwalled carbon nanotube modified electrode based on the ITO to establish the method for measuring the uric acid. The method adopts a differential pulse voltammetry to perform the electrical measuring, and adopts a standard curve method to perform the quantitative analysis. The method is simple, low in the measuring cost, and has higher accuracy and precision in measuring the uric acid, so that the method has high application value in the field of the uric acid detection.

The invention relates to method for preparing a functionalized composite nano-fiber modified electrode. The method specifically comprises the steps of (1) preparing a spinning solution; (2) preparing composite nano-fibers through electrostatic spinning so as to form a composite nano-fiber PA6-MWNTs modified electrode; (3) carrying out electrical-polymerization thionine functionalization on the composite nano fibers: soaking the composite nano-fiber PA6-MWNTs modified electrode into a polymerization solution containing thionine monomers PTH, applying a voltage to the modified electrode so as to carry out anodizing treatment, then, carrying out cyclic volt-ampere scanning, washing away the thionine monomers, adsorbed to the modified electrode, by using a phosphate buffer solution PBS, forming a functionalized composite nano-fiber electrode modification material PA6-MWNTs / PTH layer on the surface of the electrode after the polymerization reaction is ended, thus obtaining the functionalized composite nano-fiber PA6-MWNTs / PTH modified electrode. According to the method, a functionalized composite nano-fiber electrode modification material which has the characteristics of good stability, large specific surface area, good biocompatibility, high electron transfer rate, uniformly-distributed diameter and pore size, and the like is obtained.

The invention discloses a flexible L-phenylalanine electrochemical sensor based on a dendritic nanometer silver structure, and belongs to the technical field of electrochemical sensors. A flexible electrode substrate is PDMS, and a layer of nanometer conducting layer with a dendritic structure is on the PDMS substrate. The preparation method comprises the following steps: self-assembly of ITO glass is carried out; electrochemical deposition of the nanometer silver film with a dendritic structure is carried out on the ITO glass; the ITO glass with the nanometer silver conducting layer is placed in a PDMS solution for solidification; after solidification, the PDMS film is uncovered from the ITO glass, and the flexible L-phenylalanine electrochemical sensor based on the dendritic nanometer silver structure is obtained. The flexible L-phenylalanine electrochemical sensor based on the dendritic nanometer silver structure has good response and simple preparation method, quantitative production is easy to carry out, and the product is hopeful to be widely applied to the fields of industrial and agricultural production, bioscience research, especially wearable medical equipment.

The invention discloses an electroluminescent material based on a phenanthrenequinone derivative. The electroluminescent material has a chemical name of (E)-2-3-(4-(1H-phenanthro[9,10-d]imidazole-2-yl)styryl)-5,5-dimethylcyclohex-2-en-1-ylidene)malononitrile, and a molecular formula of C34H26N4. According to the invention, through introduction of a malononitrile derivative for construction of a red luminescent molecule, the disadvantage of the design of a compound containing a phenanthro[9,10-d]imidazole group in the red luminescent molecule is made up, and the compound has strong red fluorescence and good electrochemical response; under the action of an applied voltage of +1.47 V, the compound can realize electroluminescence (ECL); and under the multiplication of 800 V, the compound has luminescent intensity of about 1900 and is extremely stable. The invention also discloses a preparation method for the electroluminescent material and an application of the electroluminescent materialin preparation of organic electroluminescent devices.

The invention discloses a preparation method and application of a graphene-amphiphilic column[5]arene-gold nanoparticle ternary nanocomposite. The preparation method of the nanocomposite comprises preparation of a graphene-column[5]arene nanocomposite and preparation of graphene-column[5]arene-gold nanoparticles. The synthetic method provided by the invention is simple and quick; experiments prove that an electrode modified by the graphene-column[5]arene-gold nanoparticle ternary nanocomposite is capable of synergistically promoting the electrochemical reaction of dopamine, and has a wide linear range to electrochemical detection of dopamine, namely 1.2*10<-8>-2.5*10<-5> mol.L<-1>, and also an extremely limit of detection, namely 9*10<-9> mol.L<-1>.