Preparation of an ito-based carboxylated multi-walled carbon nanotube-modified electrode and a method for measuring uric acid using the electrode

A technology of multi-walled carbon nanotubes and modified electrodes, which is applied in the field of electrochemical analysis, can solve the problems of cumbersome sample processing, limited scope of use, and high price in chromatography, and achieve high activity of surface atoms, low cost of determination, and simple method Effect
CN109298060BActive Publication Date: 2021-03-26DALIAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN UNIV
Publication Date
2021-03-26

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Abstract

The invention relates to the technical field of the electrochemical analysis, and particularly relates to a preparation method of a carboxylated multiwalled carbon nanotube modified electrode based onITO and a method for measuring uric acid by using the electrode. An electrochemical measuring system is composed of a self-made electrode serving as a working electrode, an Ag / AgCl electrode servingas a reference electrode, a platinum wire serving as an auxiliary electrode, and an electrochemical workstation. The preparation method comprises the following steps of: taking ITO glass as a substrate, alternately self-assembling PDDA and PSS on the ITO surface to form a film, and uniformly coating a layer of carboxylated multiwalled carbon nanotube on the self-assembled layer. The invention aimsto apply the carboxylated multiwalled carbon nanotube modified electrode based on the ITO to establish the method for measuring the uric acid. The method adopts a differential pulse voltammetry to perform the electrical measuring, and adopts a standard curve method to perform the quantitative analysis. The method is simple, low in the measuring cost, and has higher accuracy and precision in measuring the uric acid, so that the method has high application value in the field of the uric acid detection.
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Description

technical field

[0001] The invention relates to the technical field of electrochemical analysis, in particular to an electrode that can be used for measuring uric acid. Background technique

[0002] The biomolecule uric acid is the final metabolite of purine metabolism in the human body. Under normal circumstances, the uric acid in the body is about 1200 mg and is in a balanced state. High uric acid concentration in the body can cause gout, cardiovascular disease, kidney disease, etc. The current main analysis methods for uric acid include high performance liquid chromatography (HPLC), sensor method, enzymatic method, electrochemical analysis method and photometric method, etc. Spectroscopic methods are easily affected by other chromophores in the sample; chromatography sample processing process Too cumbersome; although the enzymatic method has good selectivity, it is expensive and costly, which limits its scope of use. Contents of the invention

[0003] In order to mak...

Claims

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