Preparation method for biologic sensor and modified electrode based on attapulgite clay soil as substrate

A technology for attapulgite clay and biosensor, which is applied in the field of biosensor and modified electrode preparation with attapulgite as a matrix, can solve the problems of high enzyme dosage and high sensor manufacturing cost, and achieves high sensitivity, fast electronic response speed and wide source. Effect

Inactive Publication Date: 2013-03-27
HUAIYIN TEACHERS COLLEGE
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Problems solved by technology

However, since the reaction layer is superimposed in the cavity with capillary pores, the amount of enzyme used is relatively high, and the production cost of the sensor is relatively high.

Method used

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  • Preparation method for biologic sensor and modified electrode based on attapulgite clay soil as substrate
  • Preparation method for biologic sensor and modified electrode based on attapulgite clay soil as substrate
  • Preparation method for biologic sensor and modified electrode based on attapulgite clay soil as substrate

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example 1

[0037] Example 1: if Figure 1-3 As shown, the sensor is composed of an electrolytic cell 7 connected to a transducer 10 and a display 11. An electrode bracket 9 is installed on the electrolytic cell 7 storing an electrolyte 8. A modified electrode 1, a Pt sheet electrode 5 and a saturated sweetener are installed on the electrode bracket 9. Mercury electrodes 6 , each electrode is connected to a display 11 through a transducer 10 .

[0038] In the attapulgite clay-based biosensor of the present invention, the modified electrode 1 is a working electrode, the Pt sheet electrode 5 is a counter electrode, and the saturated calomel electrode 6 is a reference electrode.

[0039] In the attapulgite clay-based biosensor of the present invention, the modified electrode 1 is a Nafion-GOD / ATP / GC electrode with a glassy carbon electrode as the substrate.

[0040] In the preparation method of the modified electrode of the present invention, the glassy carbon electrode 2 is used as a subst...

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Abstract

The invention discloses a preparation method of a biosensor and a modified electrode that take the substrate of attapulgite clay, the sensor takes the substrate of the attapulgite clay with a natural nano structure, utilizes the unique pore canal structure and the excellent adsorptive power of the attapulgite clay, fixes glucose oxidase (GOD) on the surface of the attapulgite clay, coats the attapulgite clay with adsorbed oxidase on the surface of a glassy carbon electrode, and drops and coats a layer of Nafion methanol solution on the surface, thus forming the Nafion-GOD / ATP / GC modified electrode. The modified electrode, a platinum sheet electrode, a saturated calomel electrode form an electrolytic cell, which is further connected with a converter and a display screen into the biosensor. The biosensor has quick response time, high sensitivity, simple and convenient production method, good repeatability and stability, and convenient mechanization, integration and commercialization.

Description

technical field [0001] The invention relates to a biosensor and a preparation method thereof, in particular to a biosensor with attapulgite clay as a matrix and a preparation method for a modified electrode. Background technique [0002] Biosensors have been widely concerned by people in the industry for their ability to quickly, accurately and conveniently detect the content of biological samples such as glucose in the biology, food and fermentation industries. However, the current biosensors generally have shortcomings such as insufficient sensitivity, poor anti-interference ability, low stability and high production cost. For example, the Chinese patent application "whole blood glucose biosensor" (application number 03129434.0) proposes a glucose biosensor made of carboxymethyl cellulose, modified carbon nanotubes (electronic mediator), glucose oxidase and other materials in the reaction layer, Can be used to determine the content of glucose in the blood. The invention ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48G01N27/327G01N27/403G01N27/36
Inventor 徐继明张艺凡宋洁吕金顺尹起范
Owner HUAIYIN TEACHERS COLLEGE
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