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Preparation of glassy carbon electrode modified by gold nanoparticles wrapped with ionic liquid and detection on cholesterol by glassy carbon electrode

A technology of gold nanoparticles and glassy carbon electrodes, which is applied in the direction of electrochemical variables of materials, etc., to achieve the effects of high enzyme activity, good reproducibility and simple preparation process

Inactive Publication Date: 2014-07-23
NORTHWEST NORMAL UNIVERSITY
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Problems solved by technology

However, the poor compatible environment of the supporting medium and the redox active site of the enzyme are deeply buried in the protein, which limits the analytical efficiency of the prepared enzyme sensor.

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  • Preparation of glassy carbon electrode modified by gold nanoparticles wrapped with ionic liquid and detection on cholesterol by glassy carbon electrode
  • Preparation of glassy carbon electrode modified by gold nanoparticles wrapped with ionic liquid and detection on cholesterol by glassy carbon electrode
  • Preparation of glassy carbon electrode modified by gold nanoparticles wrapped with ionic liquid and detection on cholesterol by glassy carbon electrode

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[0039] In order to more clearly illustrate the content of the present invention, the technical scheme of the present invention will be further described below in conjunction with the accompanying drawings:

[0040] Used instrument and medicine in the implementation process of the present invention:

[0041] CHI 660C electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.) was used for cyclic voltammetry experiments, and a quartz tube heating automatic double pure water still (1810B, Shanghai Asia Pacific Technology Glass Co., Ltd.) was used to distill twice distilled water. A UV-Vis spectrophotometer (1102, Shanghai Tianmei Scientific Instrument Co., Ltd.) and a transmission electron microscope (JEOL JSM-6700F, Japan Optical Co., Ltd.) were used to characterize gold nanoparticles, and an electronic balance (Beijing Sartorius Instrument Co., Ltd.) was used to weigh amount of medicines. Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.). Aluminum oxi...

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Abstract

The invention provides preparation of a glassy carbon electrode modified by gold nanoparticles wrapped with ionic liquid and detection on cholesterol by the glassy carbon electrode. The preparation method comprises the following steps: at first, performing in-situ reduction on chloroauric acid by using sodium borohydride in an ionic liquid two-phase system to obtain gold nanoparticles wrapped with the ionic liquid; then, directly fixing cholesterol oxidase (ChOx) on a gold nanoparticle composite material wrapped with the ionic liquid; and finally, dropwise coating the surface of the glassy carbon electrode with the compounded composite material to form a modified glassy carbon electrode. The modified glassy carbon electrode has good electrochemical response to cholesterol and can be used for directly detecting cholesterol. The detection on cholesterol by the prepared glassy carbon electrode has the characteristics of wide linear range and low detection limitation.

Description

technical field [0001] The invention relates to the preparation of an ionic liquid-coated gold nanoparticle modified glassy carbon electrode and its detection of cholesterol. Background technique [0002] Gold nanoparticles have attracted extensive attention in the field of electrochemical sensing due to their ability to efficiently load enzymes and maintain the biological activity of enzymes. Many literatures have reported that gold nanoparticles with small particle size can accelerate the transfer of electrons generated in enzyme-catalyzed redox reactions to the electrode surface. However, when there is no protective agent or surface passivation on the surface of gold nanoparticles, the Agglomeration can easily occur, resulting in the reduction of the catalytic activity of the gold nanoparticles. The present invention uses the ionic liquid to stabilize the gold nanoparticles to obtain the gold nanoparticles with small particle size and good dispersibility. [0003] Ionic...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
Inventor 刘秀辉董明君刘盼盼李嘉伟卢小泉
Owner NORTHWEST NORMAL UNIVERSITY
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