Preparation method for biologic sensor and modified electrode based on attapulgite clay soil as substrate

The technology of attapulgite clay and biosensor is applied in the field of preparation of biosensors and modified electrodes based on attapulgite clay, which can solve the problems of high enzyme dosage and high sensor manufacturing cost, and achieves high sensitivity, fast electronic response speed and low price. Effect
CN101344522AInactive Publication Date: 2009-01-14HUAIYIN TEACHERS COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAIYIN TEACHERS COLLEGE
Publication Date
2009-01-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a biosensor and a modified electrode that take attapulgite clay as substrate, the sensor takes the attapulgite clay with natural nano structure as the substrate, and utilizes the unique pore canal structure and the excellent adsorptive capability of the attapulgite clay to fix glucose oxidase (GOD) on the surface of the attapulgite clay, coats the attapulgite clay with adsorbed oxidase on the surface of a glassy carbon electrode, and drops and coats a layer of Nafion methanol solution on the surface, thus forming the Nafion-GOD / ATP / GC modified electrode. The modified electrode, a platinum sheet electrode, a saturated calomel electrode form an electrolytic cell which is further connected with a converter and a display screen to form the biosensor. The biosensor has quick response time, high sensitivity, simple and convenient production method, good repeatability and stability, and convenient mechanization, integration and commercialization.
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Description

technical field

[0001] The invention relates to a biosensor and a preparation method thereof, in particular to a biosensor with attapulgite clay as a matrix and a preparation method for a modified electrode. Background technique

[0002] Biosensors have been widely concerned by people in the industry for their ability to quickly, accurately and conveniently detect the content of biological samples such as glucose in the biology, food and fermentation industries. However, the current biosensors generally have shortcomings such as insufficient sensitivity, poor anti-interference ability, low stability and high production cost. For example, the Chinese patent application "whole blood glucose biosensor" (application number 03129434.0) proposes a glucose biosensor made of carboxymethyl cellulose, modified carbon nanotubes (electronic mediator), glucose oxidase and other materials in the reaction layer, Can be used to determine the content of glucose in the blood. The invention ...

Claims

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