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Preparation method of DNA sensing electrode based on anionic porphyrin-carbon nano-tubes

A technology of carbon nanotubes and sensing electrodes, applied in the field of electrochemistry and nanomaterials, can solve the problems of difficult processing, lack, easy entanglement and agglomeration of surface functional groups

Inactive Publication Date: 2018-10-19
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the method for preparing high-quality CNTs in batches is becoming more and more mature. However, CNTs have their own shortcomings, such as insolubility, easy entanglement and agglomeration, and lack of surface functional groups, making it difficult to process, manipulate, disperse and stabilize. Due to the above problems, scientists are also trying to find modifiers that can improve the performance of CNTs, and then turned their attention to porphyrin compounds, which are a class of π-π conjugated rings with 4 pyrrole molecules. Compounds, with rigid planar structure and high stability, these characteristics make it molecular recognition and rich optoelectronic properties

Method used

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  • Preparation method of DNA sensing electrode based on anionic porphyrin-carbon nano-tubes
  • Preparation method of DNA sensing electrode based on anionic porphyrin-carbon nano-tubes
  • Preparation method of DNA sensing electrode based on anionic porphyrin-carbon nano-tubes

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example 1

[0015] Example 1 The present invention is used in a kind of preparation method of DNA sensing electrode based on anion porphyrin-carbon nanotube

[0016] GCE with 0.3 μm, 0.05 μm α-Al 2 o 3 Polish to a mirror surface, then ultrasonically clean with ethanol and double distilled water respectively, and dry at room temperature. The carbon nanotubes were ultrasonically dispersed in 0.05 M, pH = 8.00 Tris-HCl containing 0.1 M KCl buffer to obtain a CNTs dispersion with a mass concentration of 2.5 × 10 -3 L -1 ; Then ssDNA (2.0 × 10 -6 M) and its complementary paired ssDNA (2.0 × 10 -7 M) Add the prepared CNTs dispersion, and then add the TSPP solution (2.5 × 10 -7 M), TSPP can be adsorbed on the surface of CNTs through π-π interaction, but cannot approach CNTs due to the repulsion of ssDNA; however, dsDNA formed by the hybridization reaction falls off the surface of carbon nanotubes while TSPP is adsorbed on CNTs through π-π interaction Surface, and finally get modified e...

example 2

[0017] Example 2 Detection of the electrochemical performance of the DNA biosensing electrode prepared in the present invention.

[0018] (1) Electrochemical performance detection of different modified electrodes

[0019] Using GCE, CNTs / GCE, TSPP / GCE, and TSPP / CNTs / GCE modified electrodes as working electrodes, platinum wire as auxiliary electrode, and Ag-AgCl as reference electrode, a three-electrode system was established. On the workstation, within the potential range of -0.2~0.6V, at 0.005 M [Fe(CN) 6 ] 3− / 4− , in 0.10 M KCl electrolyte solution, its electrochemical performance was characterized by electrochemical cyclic voltammetry (CV), as figure 1 As shown, it was found that the modified electrode ( figure 1 Curve d) compared to modified electrode alone ( figure 1 Curves a, b, and c) have larger peak currents and smaller potential differences, indicating that the modified electrode has excellent conductivity, indicating that the modified electrode prepared by the...

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Abstract

The invention belongs to the technical field of electrochemical and nanometer materials, and relates to a DNA sensing electrode preparation method, wherein a DNA sensing electrode based on anionic porphyrin-carbon nano-tube modification is prepared by using the pi-pi interaction between anionic porphyrin and carbon nano-tubes. The modification steps comprise: ultrasonically dispersing carbon nano-tubes (CNTs) in a Tris-HCl buffer liquid, adding ssDNA and complementary paired ssDNA thereof (are hybridized to form dsDNA), adding an anionic porphyrin (TSPP) solution, and carrying out drop coatingto obtain the TSPP / dsDNA / CNTs / GCE modified electrode. According to the present invention, the preparation method has characteristics of simple process and short preparation time; the prepared electrode is the good detection electrode; and the prepared DNA biological sensing electrode combines the specificity of DNA complementary pairing and the high sensitivity of electrochemical detection, and is expected to provide the new way for the early diagnosis of cancer cells.

Description

technical field [0001] The invention belongs to the technical field of electrochemistry and nanomaterials, and relates to the technical field of preparation of tetrasulfophenylporphyrin (TSPP) modified electrodes, in particular to a preparation method of a DNA sensing electrode based on anion porphyrin-carbon nanotubes and This electrode is used for the detection of ssDNA. Background technique [0002] Nanomaterials technology is a multidisciplinary emerging discipline developed in the early 1990s. Its development has opened up a new level of human understanding of the world. Carbon nanotubes (CNTs) have attracted the attention of scientists due to their unique physical and chemical properties such as conductor and semiconductor properties, extremely high mechanical strength, large specific surface area and aspect ratio, and more catalytic sites. At present, the method for preparing high-quality CNTs in batches is becoming more and more mature. However, CNTs have their own ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 宁静恒刘龙王敏罗新
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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