36 results about "Ultramicroelectrode" patented technology

The invention discloses a preparation method of a dual mode (two mode signals-electrophysiological signal and electrochemical signal) ultramicro planar array sensor for quantitative assay of nerve cell quantum release. An ultramicro planar electrode (0.5-5 mum) is prepared through combination of a double layer wiring design, a high precision stepping photolithography technique and a partition isolation design. According to the method, orientated nanometer modification, biological compatibility modification and specific recognition enzyme modification are combined and performed on the surface of an electrode array so as to prepare the dual mode planar ultramicro electrode array sensor. The dual mode planar ultramicro electrode array is prepared by the method combining a micro electromechanical system technique, a nanometer modification technique and a biological modification technique. Through adoption of the method, the limitations of large electrode site size (10-50 mum) of a conventional planar microelectrode and single site detection of a rodlike carbon fiber electrode are broken. The ultramicro planar electrode array prepared by the method has small electrode site and multiple recording points, does not damage nerve cells, and can simultaneously detect neurotransmitter quantum release of a plurality of nerve cells and dual mode information of an electrophysiological action potential signal in real time in situ.

The method of preparing reconfigurable ultramicroelectrodes with controllable morphology based on magnetic field drive first prepares a substrate with a micron groove array and a liquid storage tank; selects a low-melt alloy material as the electrode material, and adds iron powder; the substrate is placed in the storage tank. In the liquid tank, add hydrochloric acid solution into the liquid storage tank, and inject liquid low-melting alloy on the groove of the substrate; the motion control platform controls the permanent magnet to rise to be in contact with the liquid storage tank, and align the permanent magnet with the low-melting alloy droplet. The permanent magnet moves according to the pre-designed electrode pattern, and the low-melting alloy droplet is driven by the permanent magnet to form the electrode pattern; the substrate is taken out from the liquid storage tank, cooled, and the shape-controllable reconfigurable ultramicro Electrode; the reconfigurable ultramicro electrode is heated, the reconfigurable ultramicro electrode is melted, and the permanent magnet is used to absorb the low-melt alloy droplet from the substrate, that is, the reconstruction of the low-melt alloy reconfigurable ultramicro electrode is completed; the present invention can The electrode with complex pattern shape is manufactured, the preparation method is simple, and the electrode material can be recycled.

The invention discloses a preparation method of a gold disc sub-micron electrode with a controllable geometrical shape. The method mainly comprises the following steps: placing a gold wire into a capillary glass tube, the capillary glass tube is firstly refined by use of a laser drawing instrument, then the capillary glass tube is enabled to be completely attached to the gold wire, finally, the capillary glass tube is snapped off, an ultramicroelectrode is obtained, then the tip of the ultramicroelectrode is heated by use of a needle grinding instrument, through controlling the heating process, the diameter of the capillary glass tube and the RG of the electrode are controlled, finally, the cross section of the tip of the ultramicroelectrode is truncated by use of a focusing icon beam electron microscope, and thus the gold disc sub-micron electrode is obtained. The electrode obtained by use of the method has the following advantages: the end surface is flat, the insulation layer is uniform and compact, the sensitivity is high, and the electrochemical performance is excellent. Therefore, the electrode is especially applied to basic electrochemistry, biological electrochemical sensors and scanning probes.

The invention discloses a preparation method for a Pt disk sub-micron electrode with a controllable geometrical shape. The method mainly comprises the steps of placing a Pt wire in a capillary glass tube; thinning a middle part of the capillary glass tube through adoption of a laser drawing device; enabling the capillary glass tube to fit with the Pt wire completely; breaking the capillary glass tube to obtain ultramicro electrodes; heating the tips of the ultramicro electrodes through adoption of a needle grinding device; controlling the diameters of the capillary glass tubes and electrode RG by controlling the heating process; and cutting off the sections of the tips of the ultramicro electrodes through adoption of a focused ion beam electron microscope, thereby obtaining the Pt disk sub-micron electrodes. According to the method, the obtained Pt sub-micron electrodes are flat in end face, uniform and densified in insulation layer, high in sensitivity and excellent in electrochemical performance and are especially applicable to basic electrochemistry and bioelectrochemistry sensors and scanning probes.

The invention discloses a simple and fast method for preparing an ultramicro electrode array, which relates to a method for preparing an ultramicro electrode array. The purpose of the present invention is to solve the problems of complex process, high cost and time-consuming preparation of the existing ultramicro electrode array. Method: 1. Paste one side of the double-sided carbon conductive tape flatly on the metal-plated glass sheet; 2. Place a template with nano-aperture on the other side of the double-sided carbon conductive tape to obtain the assembled metal sheet; 3. Open a round hole at the bottom of the electrolytic cell, and then place the assembled metal sheet on the bottom of the electrolytic cell; 4. Use constant voltage deposition under room temperature and nitrogen atmosphere conditions to obtain super microelectrode array. The invention is convenient and fast, has simple process, low cost, environmental protection and high efficiency, and can prepare various metal nanostructures at the same time. The invention can obtain an ultramicro electrode array.

The invention belongs to the fields of preparation method and application of microelectrodes, and provides a nano flower-shaped ultramicro gold electrode and a preparation method and an application thereof. Micro carbon fibers and copper wires are adhered by a graphite conductive adhesive and then extend into a glass capillary tube with an end part stretched, and flame etching is performed to obtain a nanoscale diameter; the surface is subjected to electrochemical deposition with an electrophoretic paint, and heating and baking treatment is performed; in an acidic chloroauric acid solution, the nano flower-shaped ultramicro gold electrode is obtained by electrochemical deposition of gold. The surface of the prepared electrode is assembled with biological molecules marked with an electrochemical active substance, a receptor of the surface of a target molecule can be specifically recognized, and the target molecule is detected by an electrochemical method. The electrode can be applied in the research fields of biological sensors, biological molecular detection and the like. The preparation method has the advantages of simpliness, low cost, small size and easy operation; and the surface of the prepared gold electrode is clean, the specific surface area of the ultramicro electrode is increased, and an electrochemical signal is enhanced.

The invention discloses a method for preparing metal hybrid and homogeneous ultramicro electrodes by using carbon template and electroplating method. The method can prepare single-tube ultrafine metal electrodes including gold, platinum, mercury, etc., and double-channel hybrid and homogeneous ultrafine metal electrodes with different (or the same) metal materials on both sides. Firstly, micro-nano-scale quartz double tubes are drawn according to the needs of the experiment, and then high-temperature cracking and deposition of butane is performed on the dual channels of the quartz tubes to prepare incompletely sealed carbon templates. Since no special attention to nitrogen protection is required, the method has greatly reduced requirements on operation and environment. On this basis, we inserted it into a metal electrolyte containing a certain concentration, determined the reduction potential and performed constant potential electroplating, and finally obtained various metal ultramicro electrodes with dense and flat filling tips. Since the outer wall is silanized, metal growth outside the tube is prevented. Electrochemical techniques and surface analysis techniques were used to characterize the prepared electrodes.

The invention discloses a method for regenerating carbon fiber ultramicroelectrode. The method is characterized in that a reference electrode and a poisoning electrode are placed in deionized water, through a constant potential current time method, the reference electrode and the poisoning electrode are activated, wherein no chemical substance is added in the deionized water. The above method has good activation effect, compared with the poisoning electrode, the electrochemistry response of the processed carbon fiber ultramicroelectrode is obviously increased, and the electrochemistry performance of the regenerated electrode is stable.

The present invention discloses a method of manufacturing an ultra-microelectrode and is characterized by sealing the top end of a capillary tube by adopting a flame fusing method, plating a dielectric film by adopting a cyclic voltammetry method for the first time and obtaining the top end of an ultra-microelectrode by using an electric shock method. Comparing with the traditional technology, the present invention discloses an ultra-microelectrode having a top end with a minimum diameter of 300-1000nm, having a uniform compact uneasy-falling dielectric film, and the size is controllable, thesensitivity is high, the steady-state current is reduced from 10[-7]A to 10[-9]A, the ultra-microelectrode can be tested in an organic system, and thus, application fields of the ultra-microelectrodeis widened greatly. The method has characteristics of simple manufacturing and low cost, and the manufactured ultra-microelectrode has a perfect electrochemical performance and can be used for singlecell detecting and probes of scanning electrochemical microscopes.