35 results about "Ultramicroelectrode" patented technology

The invention discloses a preparation method of a dual mode (two mode signals-electrophysiological signal and electrochemical signal) ultramicro planar array sensor for quantitative assay of nerve cell quantum release. An ultramicro planar electrode (0.5-5 mum) is prepared through combination of a double layer wiring design, a high precision stepping photolithography technique and a partition isolation design. According to the method, orientated nanometer modification, biological compatibility modification and specific recognition enzyme modification are combined and performed on the surface of an electrode array so as to prepare the dual mode planar ultramicro electrode array sensor. The dual mode planar ultramicro electrode array is prepared by the method combining a micro electromechanical system technique, a nanometer modification technique and a biological modification technique. Through adoption of the method, the limitations of large electrode site size (10-50 mum) of a conventional planar microelectrode and single site detection of a rodlike carbon fiber electrode are broken. The ultramicro planar electrode array prepared by the method has small electrode site and multiple recording points, does not damage nerve cells, and can simultaneously detect neurotransmitter quantum release of a plurality of nerve cells and dual mode information of an electrophysiological action potential signal in real time in situ.

The invention relates to a preparation method and application of a core-shell type ultra-micro electrode. The core-shell structure ultra-micro electrode is obtained by preparing ultra-micro electrode fibers of a coaxial structure in a one-step mode through a coaxial electrostatic spinning method and then performing packaging. The coaxial structure comprises a core electrode base layer and one or two surface sensing layers. By the adoption of the one-step forming technology, the complex surface modification process of traditional ultra-micro electrodes (such as glassy carbon electrodes obtained after ablation treatment) is omitted, and the ultra-micro electrode with the diameter ranging from 40 nm to 6 micrometers can be prepared; due to the subsequent treatment temperature ranging from 70 DEG C to 150 DEG C, the structures and functions of organic functional components (such as biological protein and enzymes) in a modification layer of the ultra-micro electrode can be easily maintained, which is of great significance for the pluralistic design of the ultra-micro electrode. The ultra-micro electrode is used for detecting adenosine triphosphate (ATP), dopamine and adrenal hormones and is high in response speed and sensitivity, strong in anti-jamming capability and especially suitable for on-line rapid determination, particularly, real-time non-destructive determination of living cells.

The invention belongs to the fields of preparation method and application of microelectrodes, and provides a nano flower-shaped ultramicro gold electrode and a preparation method and an application thereof. Micro carbon fibers and copper wires are adhered by a graphite conductive adhesive and then extend into a glass capillary tube with an end part stretched, and flame etching is performed to obtain a nanoscale diameter; the surface is subjected to electrochemical deposition with an electrophoretic paint, and heating and baking treatment is performed; in an acidic chloroauric acid solution, the nano flower-shaped ultramicro gold electrode is obtained by electrochemical deposition of gold. The surface of the prepared electrode is assembled with biological molecules marked with an electrochemical active substance, a receptor of the surface of a target molecule can be specifically recognized, and the target molecule is detected by an electrochemical method. The electrode can be applied in the research fields of biological sensors, biological molecular detection and the like. The preparation method has the advantages of simpliness, low cost, small size and easy operation; and the surface of the prepared gold electrode is clean, the specific surface area of the ultramicro electrode is increased, and an electrochemical signal is enhanced.

A method consists in measuring ac impedance spectrum using gold, platinum or carbon working ultramicroelectrodes (1). The direct current DC potential pulse which overlaps with alternating current AC signal of an amplitude in the range from 0.005 to 0.015 V and frequency in the range from 10<-2 >to 10<6>, Hz is applied at the electrode. The value of said DC potential pulse for a process of copper electrorefining is in the range from -0.4 to -0.9 V in relation to a platinum reference electrode (2). Resistance values characteristic of a given electrolyte (E) are obtained from said AC impedance spectrum and compared with standard characteristic obtained by standard additions method. A measuring system consist of electrodes (1, 2, 3) placed inside a measuring cell (4) filled with flowing industrial electrolyte. The electrodes (1, 2, 3) are joined to a spectrum analyser (12) controlled by a programming apparatus (13)

The invention provides an integrated microfluidic electrochemical sensor chip for rapid BOD detection, and a preparation method and a BOD detection method thereof, and relates to the technical field of BOD detection. The chip comprises: a monocrystalline silicon substrate; a PDMS layer superposed inside the surface of the monocrystalline silicon substrate and provided with a microfluidic channel system, wherein the microfluidic channel system comprises a to-be-detected liquid inlet, a microfluidic channel, a biofilm reactor, an electrochemical detection pool and a waste liquid outlet; a three-electrode system arranged between the monocrystalline silicon substrate and the PDMS layer and arranged in an interdigital ultramicro electrode array; and a platinum film temperature sensor arranged on the bottom surface of the monocrystalline silicon substrate, wherein a micro heating copper electrode is adhered to the surface of the platinum film temperature sensor. Effective integration of twofunctional units of the biofilm reactor and the electrochemical electrode is realized on the same chip by adopting a microfluidic technology, and miniaturization of the sensor and rapid, stable, accurate and continuous automatic monitoring of the BOD of the water sample are realized.

The invention discloses an electrochemical ultra-micro compound electrode and a preparation method thereof. The method adopts optical lithography; after a pattern of an ultra-micro electrode is transferred onto a substrate, photoresist is thrown and coated again, and is directly taken as an insulation encapsulating layer; and the photoresist on the tip part of the electrode is etched through exposure to form a micro electrolyzer. The method has simple manufacturing steps, can massively prepare electrode at one time, and ensures accurately controllable electrode exposing areas. The micro electrolyzer prepared has good electrochemical characteristics, such as high mass transfer rate, high signal to noise ratio, high current density and the like in experimental tests. The electrode has fixed position, so a result with good repeatability can be obtained. The electrolyzer has a capacity of less than 1pL, and can be used for accurately confining and detecting trace electrolyte. The results show that the devices have good trace detection capacity.

The invention relates to an ultra-fine cone electrode array and a method for manufacturing the same. The method for manufacturing the nano-size ultra-fine cone electrode array comprises the following steps: oxidizing a silicon slice with the wet process and the dry process to form an SiO2 film on the silicon slice, and photoetching a rectangular window on the SiO2 film by using positive photoresist; etching the silicon slice exposed in the rectangular window on the SiO2 film with the wet process by using the TMAH solution to form a pit in the rectangular pyramid shape, and removing the left SiO2 film; thermally oxidizing the silicon slice at 950 DEG C to form an SiO2 film, sputtering ultra-fine electrode material on the surface of the silicon slice, which is provided with the pit, with the radio-frequency magnetron sputtering process, and forming a ultra-fine electrode position and a lead wire pattern with the photoetching process and the etching process; depositing an Si3N4 film on the surface of the ultra-fine electrode material with the vapor-phase deposition process, and photoetching and etching a window on the Si3N4 film with the photoetching process and the etching process so as to expose an ultra-fine electrode bonding window; thinning and etching the back surface of the silicon slice by using the TMAH solution until an SiO2 lug boss being exposed, and etching the SiO2 lug boss until ultra-fine electrodes with a certain height ratio being exposed.

The invention discloses an integrated ultramicro electrode, a preparation method thereof and application. According to the integrated ultramicro electrode, an ultramicro working electrode and a counter electrode are integrated in a theta-type glass microtube, so that a two-electrode system can be formed; the minimum iR drop of the ultramicro electrode provides a basis and guarantee for the construction of the two-electrode system. According to an integrated ultramicro electrode probe, only microliters of solution is needed in single measurement, so that the consumption of samples is greatly reduced; meanwhile, the spatial resolution, portability and on-site detection capability of the probe are enhanced, so that the probe is suitable for the in-situ electrochemical detection of electroactive substances, enzyme activity and biological samples in a homogeneous solution with any microliter-level volume; a test process is not limited by the volume and measurement time of a supporting electrolyte solution in a three-electrode system micro electrochemical detection device; the assistance of the milliliter-level supporting electrolyte solution is not needed, measurement can be directly carried out, and therefore, detection efficiency is improved.

The invention discloses a method for quantitatively measuring serotonin. The method comprises the following steps: a differential pulse voltammetry method is adopted, a clean carbon fiber ultramicroelectrode subjected to flame-burned activation is taken as a working electrode, peak current of an original clean electrode in a reference solution is taken as reference current, the quotient obtained by dividing the peak current of the original clean electrode by peak current of an activated electrode is taken as a current correction factor, and the quotient obtained by dividing the correction factor by oxidation peak current in a serotonin solution of each gradient concentration is taken as the corrected oxidation peak current; or different carbon fiber ultramicroelectrodes with the differential pulse response current differences within 20 percent in the reference solution are taken as working electrodes, the ratio of peak current of each electrode to the peak current of a first electrode is taken as a current correction factor, and the quotient obtained by dividing the correction factor by oxidation peak current in the serotonin solution of each gradient concentration is taken as the corrected oxidation peak current. A standard curve is drawn by taking the serotonin concentration as a horizontal ordinate and taking the corrected oxidation peak current as a vertical coordinate, the linear relation of the oxidation peak current after current correction and the concentration is good, and the quantitative determination of serotonin is realized.

Disclosed herein are systems and methods that relate to use of ultramicroelectrodes (UME) in measurement of ion concentration in presence of one or more organic compound(s).

The invention discloses a method for simply and rapidly preparing an ultramicro electrode array, and relates to a preparation method of an ultramicro electrode array. The invention aims to solve the problems of complex process, high cost and time-consuming preparation of an existing ultramicro electrode array preparation process. The method comprises the following steps of: 1, flatly adhering one side of a double-sided carbon conductive adhesive tape to a metal-plated glass sheet; 2, placing a template with a nanometer aperture on the other side of the double-sided carbon conductive adhesive tape to obtain an assembled metal sheet; 3, forming a round hole in the bottom of an electrolytic tank, and then placing the assembled metal sheet at the bottom of the electrolytic tank; and 4, performing constant-voltage deposition under the conditions of room temperature and nitrogen atmosphere to obtain the ultramicro electrode array on the assembled metal sheet. The method is convenient and rapid to implement, simple in process, low in cost, environment-friendly and efficient, and meanwhile various metal nanostructures can be prepared. According to the method, the ultramicro electrode array can be obtained.

The invention discloses a method for preparing a metal hybrid and homogeneous ultramicro electrode by using a carbon template and an electroplating method. The method can be used for preparing single-tube ultramicro metal electrodes such as gold, platinum, mercury and the like and double-channel hybrid and homogeneous ultramicro metal electrodes made of different (or same) metal materials on the two sides. The method comprises the following steps: firstly, drawing micro-nano quartz double tubes according to experimental requirements, and then carrying out butane high-temperature cracking deposition on double channels of the quartz tubes to prepare an incompletely sealed carbon template. As nitrogen protection does not need to be specially paid attention to, the requirements of the method on operation and environment are greatly reduced. On the basis, the electrode is inserted into a metal electrolyte with a certain concentration, constant-potential electroplating is carried out after the reduction potential is determined, and finally various metal ultramicroelectrodes with compact and flat filling tips are obtained. As the outer wall is silanized, metal is prevented from growing outside the tubes. The prepared electrode is characterized by adopting an electrochemical technology and a surface analysis technology.

The invention relates to a monomolecular protein detection chip and a monomolecular protein detection method based on ultramicro electrode array electrochemiluminescence. The monomolecular protein detection chip is provided with a substrate of an ITO (indium tin oxide) electrode and an inclined micro trap array with a three-dimensional concave part on the ITO electrode, the two ends of the inclined micro trap array in the transverse direction are respectively provided with wires connected with the positive electrode and the negative electrode of a power supply. The single-molecule protein detection chip is simple to prepare, an electrochemical luminescence detection system further amplifies a signal, and high-sensitivity single-molecule detection of trace protein can be realized.