Ultramicroelectrode preparation system for electrophysiological recording of individual nerve cells

Abstract

The invention provides an ultramicroelectrode preparation system for the electrophysiological recording of individual nerve cells, which comprises a constant voltage source, a step motor, an electrolytic cell, a digital ampere meter and a control chip; the control chip controls the constant voltage source to apply preset voltage on a microelectrode submerged in electrolyte solution, so that the microelectrode is electrochemically corroded; the step motor is controlled to drive the microelectrode to go up in the electrolyte solution, and by controlling the speed of the step motor, the tip of the microelectrode is corroded to form a taper; the detection result of the digital ampere meter is acquired, when the current change on the microelectrode is judged to reach a preset condition, the constant voltage source is switched off, the step motor is controlled to speed up to lift the microelectrode out of the electrolyte solution, and thereby an ultramicroelectrode, which is formed by microelectrode corrosion and the tip diameter of which is less than 1 micron, is obtained. The ultramicroelectrode preparation system remarkably increases the precision and speed of preparation, shortens the time of preparation, and helps to popularize the application of ultramicroelectrodes in the fields of individual nerve cell recording and fruit fly olfactory unit receptor recording.

Description

technical field [0001] The invention relates to the field of medical device manufacturing, in particular to an ultramicro electrode preparation device for electrophysiological recording of single nerve cells. Background technique [0002] At present, the increasing incidence of brain diseases and the increasing medical burden have aroused widespread concern in society, and brain science has become a current research hotspot. In order to improve neuropsychiatric diseases such as schizophrenia, epilepsy, Parkinson's disease, anorexia nervosa, depression, Alzheimer's disease, tremor, convulsions, obsessive-compulsive disorder, anxiety disorder, stroke, and drug addiction, which have always threatened human health In the current situation of diagnosis and treatment, it is urgent to study the neural circuit mechanism comprehensively and in-depth, and to explore more effective clinical treatment targets. Electrophysiology is an important research method in modern neuroscience res...

AI Technical Summary

Problems solved by technology

This will not only consume a lot of experimental time, but also very likely to damage the electrode due to improper parameter control

Existing technologies and devices have significant disadvantages that severely limit their use

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