A kind of method for quantitative determination of serotonin

A technology for the quantitative determination of serotonin, applied in the direction of material electrochemical variables, etc., can solve the problems of difficult control of film thickness, complicated electrode modification process, insufficient attachment of modifiers, etc., achieve a good linear relationship, and overcome the contamination of electrodes by serotonin oxidation products , Realize the effect of quantitative determination and detection

Inactive Publication Date: 2017-05-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

Five serotonin is an electroactive substance that can be detected by electrochemical methods, but the oxidation active product produced during the oxidation process is easily adsorbed on the surface of electrodes of various materials to form an insulating film, including noble metal electrodes, glassy carbon electrodes, and carbon fiber electrodes. And Peng doped diamond electrodes, etc., resulting in the contamination of the electrode surface and affecting the detection sensitivity and stability
Hashemi et al. used the electroplating method to modify the cation exchange polymer Nafion on the surface of carbon fiber microelectrodes (Voltammetric detection of 5-hydroxytryptamine release in the rat brain, Anal Chem, vol.81:9462-9471, 2009), which was used for the release of serotonin in the rat brain. Medical monitoring; Zhu Mingfang et al. prepared graphene-tetrasulfonic acid group iron phthalocyanine modified carbon fiber microelectrodes (Research on new methods for electrochemical determination of neurotransmitter molecules, South China University of Technology Ph.D. Dissertation, 43-56, 2012), It is also used for the simultaneous determination of dopamine and serotonin in the hippocampus of mice. However, the process of modifying the electrode is more complicated and pollutes the environment, and the modification of the electrode often has the disadvantages that the modification is not firmly attached to the electrode surface, and the thickness of the film is difficult to control. Disadvantages such as short life

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  • A kind of method for quantitative determination of serotonin
  • A kind of method for quantitative determination of serotonin
  • A kind of method for quantitative determination of serotonin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 carbon fiber ultramicro electrode preparation

[0045] One end of a clean glass capillary (1 mm inner diameter) was drawn to a tip with an inner diameter of approximately 20 μm. Carbon fibers (diameter 6 μm, length about 15 mm) were ultrasonically cleaned with acetone, ethanol, and double distilled water for 5 minutes in sequence, and dried in air. The carbon fiber and copper wire (0.2 mm in diameter, about 10 cm in length) were bonded with carbon powder conductive adhesive, the carbon fiber was penetrated from the other end of the glass capillary to about 2 mm outside the tip of the glass capillary, and the other end of the capillary was sealed with epoxy resin, statically Fix the copper wire after the epoxy resin cures overnight. Place the tip of the capillary with carbon fiber at the outer flame in the middle of the alcohol lamp (the temperature is between 750 and 850°C) and burn for about 0.5s, the tip of the capillary melts and seals the carbon fiber ...

Embodiment 2

[0046] Example 2 Detection of serotonin without activation of the electrode by flame burning

[0047] The carbon fiber ultramicroelectrodes prepared in Example 1 were cleaned in acetone, dehydrated alcohol and ultrapure water for 3min respectively, and placed in 0.5mol / L H 2 SO 4 The aqueous solution is activated by cyclic voltammetry for 0.5 h, and then cleaned with ultrapure water to obtain a clean carbon fiber ultramicro electrode after activation.

[0048] Place a clean carbon fiber ultramicro electrode at 1.0×10 -4 The mol / L serotonin solution was subjected to cyclic voltammetry scans 10 times, the scan speed was set at 100mV / s, and the scan range was from 0.1V to 0.8V. The current decreases as the number of scans increases, such as figure 1 As shown, it shows that serotonin has a serious poisoning effect on the electrode surface by cyclic voltammetry. A two-electrode system was adopted, and all potentials were used as a reference electrode with Ag / AgCl.

[0049] Pla...

Embodiment 3

[0051] (1) Flame burning activated carbon fiber ultra-micro electrode method: Press the wick of the alcohol lamp with tweezers to make the flame of the alcohol lamp show a weak flame, and then slowly approach the outer flame at the bottom of the flame (the temperature is about 350 ℃), remove it immediately when observing the carbon fiber tip slightly red against the black background.

[0052] (2) Place a clean carbon fiber ultramicro electrode at 1.0×10 -4 mol / L dopamine and 1.0×10 -3 mol / L potassium ferricyanide solution for cyclic voltammetry scanning and differential pulse voltammetry scanning, after being poisoned by serotonin, place it in the above solution for scanning, activate the carbon fiber ultramicro electrode by flame burning method, and then place it Scan in the above solution. The cyclic voltammetry and differential pulse diagrams of carbon fiber ultramicroelectrodes in each electroactive material before and after poisoning and after activation are shown in ...

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Abstract

The invention discloses a method for quantitatively measuring serotonin. The method comprises the following steps: a differential pulse voltammetry method is adopted, a clean carbon fiber ultramicroelectrode subjected to flame-burned activation is taken as a working electrode, peak current of an original clean electrode in a reference solution is taken as reference current, the quotient obtained by dividing the peak current of the original clean electrode by peak current of an activated electrode is taken as a current correction factor, and the quotient obtained by dividing the correction factor by oxidation peak current in a serotonin solution of each gradient concentration is taken as the corrected oxidation peak current; or different carbon fiber ultramicroelectrodes with the differential pulse response current differences within 20 percent in the reference solution are taken as working electrodes, the ratio of peak current of each electrode to the peak current of a first electrode is taken as a current correction factor, and the quotient obtained by dividing the correction factor by oxidation peak current in the serotonin solution of each gradient concentration is taken as the corrected oxidation peak current. A standard curve is drawn by taking the serotonin concentration as a horizontal ordinate and taking the corrected oxidation peak current as a vertical coordinate, the linear relation of the oxidation peak current after current correction and the concentration is good, and the quantitative determination of serotonin is realized.

Description

technical field [0001] The invention relates to the technical fields of analytical chemistry, electroanalytical chemistry and bioelectroanalytical chemistry, in particular to an electrochemical detection method for quantitatively measuring neurotransmitter serotonin. Background technique [0002] Five serotonin (5-HT) is one of the main neurotransmitters in the brain. It is synthesized in the brain nerve cells and can participate in the regulation of various physiological functions and pathological states, such as body temperature, sleep, diet, and mental and emotional diseases. Five serotonin is an electroactive substance that can be detected by electrochemical methods, but the oxidation active product produced during the oxidation process is easily adsorbed on the surface of electrodes of various materials to form an insulating film, including noble metal electrodes, glassy carbon electrodes, and carbon fiber electrodes. And Peng doped diamond electrodes, etc., resulting i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 程寒杨沫马婷婷王慧霞
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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