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Integrated microfluidic electrochemical sensor chip for rapid BOD detection, and preparation method and BOD detection method thereof

A sensor chip and electrochemical technology, applied in chemical instruments and methods, laboratory containers, electrochemical variables of materials, etc., can solve problems such as inability to realize continuous online monitoring of BOD, large volume, and limited efficiency of microbial degradation of organic matter

Active Publication Date: 2020-07-31
BEIJING INFORMATION SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of instrument and method is simple to operate and detects quickly (usually within 30 minutes), it also has its own problems: first, dissolved oxygen is used as the detection object, because the solubility of dissolved oxygen in water is very low, making microorganisms degrade organic matter The efficiency is limited, resulting in a weak oxygen electrode signal, which brings challenges to the sensor back-end circuit detection system; second, the compact microbial film covers the surface of the oxygen electrode, and the replacement of the electrode will affect the microbial system at the same time, and the long-term stability of the sensor is difficult. Guaranteed, continuous online monitoring of BOD cannot be realized; third, due to the thin thickness of the microbial film on the surface of the fixed oxygen electrode, the types and quantities of microorganisms on the film are limited, and it is easy to encounter organic matter that exceeds the capacity of fixed microorganisms in practical applications. components, causing problems with low test results
However, both the traditional bioreactor and the currently proposed biofilm reactor have complex structures and large volumes, making it difficult to miniaturize detection instruments

Method used

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  • Integrated microfluidic electrochemical sensor chip for rapid BOD detection, and preparation method and BOD detection method thereof
  • Integrated microfluidic electrochemical sensor chip for rapid BOD detection, and preparation method and BOD detection method thereof
  • Integrated microfluidic electrochemical sensor chip for rapid BOD detection, and preparation method and BOD detection method thereof

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Experimental program
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preparation example Construction

[0072] The present invention provides a method for preparing an integrated microfluidic electrochemical sensor chip described in the above scheme, comprising the following steps:

[0073] (1) Insulate both sides of the single crystal silicon substrate, apply a positive photoresist on the surface of the insulated single crystal silicon substrate, and construct a three-electrode gold interdigitated ultramicroelectrode array on the surface of the insulated single crystal silicon substrate;

[0074] Form the bottom surface of the single-crystal silicon substrate of the three-electrode gold interdigitated ultramicroelectrode array with a positive photoresist, and then build a platinum thin-film temperature sensor on the bottom surface of the insulated single-crystal silicon. Surface-attached miniature heating copper electrodes;

[0075] (2) Throw a negative photoresist on the surface of the quartz glass sheet, and use photolithography to engrave the microfluidic channel system patt...

Embodiment 1

[0115] Preparation of integrated microfluidic electrochemical sensor chip:

[0116] The schematic diagram of the integrated microfluidic electrochemical sensor chip is shown in figure 1 with figure 2 As shown, the actual picture is as Figure 7 As shown, including a single crystal silicon substrate 1;

[0117]The PDMS layer 3 superimposed on the surface of the monocrystalline silicon substrate 1 (thickness is 270 μm); the inside of the PDMS layer 3 is provided with a microfluidic channel system, and the microfluidic channel system includes the inlet 4 of the liquid to be tested (divided into the liquid inlet 4-1 and the test liquid inlet 4-2), the biofilm reactor 6 (in the to-be An air injection port 10) is arranged between the inlet of the measuring liquid and the biofilm reactor, the electrochemical detection cell 7 communicating with the outlet of the inlet and the biofilm reactor through the second microflow channel 5-2, and the electrochemical detection cell 7 communi...

Embodiment 2

[0135] The integrated microfluidic electrochemical sensor chip packaged in Example 1 is used to measure the biochemical oxygen demand (BOD) of the water sample:

[0136] (1) Preparation of solution: Dilute 100mg / L BOD standard solution with 5mM PBS (pH 7.0) to prepare BOD standard solutions of different concentrations (4mg / L, 20mg / L, 40mg / L, 60mg / L and 80mg / L) , and then mixed 40mM potassium ferricyanide (formed in 5mM PBS (pH 7.0)) solution respectively, and finally prepared into BOD sample solutions of different concentrations containing 20mM potassium ferricyanide (2mg / L, 10mg / L, 20mg / L, 30mg / L and 40mg / L);

[0137] (2) Inject solution: open the liquid to be tested inlet 4-1, the liquid to be tested inlet 4-2 and the air injection port 10, close the waste liquid outlet 8, and the standard solution is simultaneously injected by the liquid to be tested inlet 4-1, the liquid to be tested Inject into the inlet 4-2 until the biofilm reactor is full of solution, close the inlet...

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Abstract

The invention provides an integrated microfluidic electrochemical sensor chip for rapid BOD detection, and a preparation method and a BOD detection method thereof, and relates to the technical field of BOD detection. The chip comprises: a monocrystalline silicon substrate; a PDMS layer superposed inside the surface of the monocrystalline silicon substrate and provided with a microfluidic channel system, wherein the microfluidic channel system comprises a to-be-detected liquid inlet, a microfluidic channel, a biofilm reactor, an electrochemical detection pool and a waste liquid outlet; a three-electrode system arranged between the monocrystalline silicon substrate and the PDMS layer and arranged in an interdigital ultramicro electrode array; and a platinum film temperature sensor arranged on the bottom surface of the monocrystalline silicon substrate, wherein a micro heating copper electrode is adhered to the surface of the platinum film temperature sensor. Effective integration of twofunctional units of the biofilm reactor and the electrochemical electrode is realized on the same chip by adopting a microfluidic technology, and miniaturization of the sensor and rapid, stable, accurate and continuous automatic monitoring of the BOD of the water sample are realized.

Description

technical field [0001] The invention relates to the technical field of biochemical oxygen demand detection, in particular to an integrated microfluidic electrochemical sensor chip for rapid detection of BOD, its preparation and a BOD detection method. Background technique [0002] Biochemical oxygen demand (also known as BOD) is a comprehensive index indicating the content of aerobic pollutants such as organic matter in water. It shows the total amount of dissolved oxygen consumed in the process of oxidative decomposition of organic matter in water due to the biochemical action of microorganisms. Its unit Expressed in mg / L, the higher the value, the more organic pollutants in the water and the more serious the pollution. The determination of BOD is to simulate the biodegradation and aerobic biochemical treatment process of natural water bodies. It is the main means to evaluate the biochemical treatment of sewage, and it is also a must-test item for water quality evaluation. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N33/18B01L3/00
CPCG01N27/26G01N33/1806B01L3/502707
Inventor 胡敬芳周兴辉胡世炜宋钰高国伟
Owner BEIJING INFORMATION SCI & TECH UNIV
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