Direct electrochemical method for detecting aesculin and/or aesculetin

A technology of quincetin and quincetin, which is applied in the field of direct electrochemical method of cerequinone and/or cerequinone, can solve the problems of long detection time, expensive detection technology equipment, complicated sample pretreatment, etc., and achieve detection Low limit, improved sensitivity and selectivity, and accurate detection results

Pending Publication Date: 2022-03-25
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of expensive equipment, complex sample pretreatment, and long detection time in the existing detection technology, the present invention provides a direct electrochemical method for th

Method used

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  • Direct electrochemical method for detecting aesculin and/or aesculetin
  • Direct electrochemical method for detecting aesculin and/or aesculetin
  • Direct electrochemical method for detecting aesculin and/or aesculetin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of electrochemical sensor PtNPs / AuNPs / CFME

[0034] 1.1 Synthesis of AuNPs

[0035] Reduction of HAuCl by trisodium citrate 4 ·3H 2 O to prepare AuNPs. 500μL (25.4mM) of HAuCl 4 ·3H 2 Add O solution and 900 μL (0.1 M) trisodium citrate solution into 35 mL of boiling ultrapure water, stir vigorously, and reflux for 40 min until the solution turns wine red. Finally, the obtained AuNPs sol was stored in a refrigerator at 4 °C.

[0036] 1.2 Synthesis of PtNPs

[0037] Reduction of H by sodium citrate 2 PtCl 6 ·6H 2 O to prepare PtNPs. 2.5mL (0.193mM) H 2 PtCl 6 ·6H 2 O was added to 42 mL (80°C±0.5°C) of water, and stirred vigorously. Quickly add 7.5mL (0.1m) trisodium citrate solution to the solution and keep stirring. The solution was refluxed for 6 h at 80 °C ± 0.5 °C until the PtCl 6 2- The characteristic absorption peaks of PtNPs disappeared completely, and the orange PtNPs sol was obtained, which was stored in a brown bottle at 4...

Embodiment 2

[0053] Example 2 The detection effect of PtNPs / AuNPs / CFME on fenceretine and fenperetin in samples

[0054] 2.1 The effect of pH value on the oxidation peak current value of fenceretin and terperetin B

[0055] The electrochemical response signals at different pH values ​​were detected by DPV.

[0056] Such as figure 2 As shown in middle E, the oxidation peak currents of teretin and teretin B gradually increased with the decrease of pH value in the range of pH 7.00 to pH 4.00, and the oxidation peak current reached the maximum at pH 4.00. Therefore, PBS buffer with a pH value of 4.00 was selected for subsequent experiments.

[0057] At the same time, with the increase of pH value, the oxidation peak potentials of fencetin and fencetin shifted negatively, indicating that protons participated in the redox process.

[0058] Oxidation peak potential E pa relationship with pH, ​​such as figure 2 As shown in F, the E pa1 and the linear regression equation between pH is E pa...

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Abstract

The invention discloses a direct electrochemical method for detecting aesculin and/or aesculetin, which is characterized in that a sample is detected by using a carbon fiber electrode PtNPs/AuNPs/CFME modified by AuNPs and PtNPs through cyclic voltammetry or differential pulse voltammetry. The electrode used in the method has good electrochemical response, one component or both can be accurately detected in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with two samples, detection results are accurate, detection limit is low, and detection sensitivity and selectivity can be improved in complex biological matrixes.

Description

technical field [0001] The invention relates to the technical field of quantitative detection of compounds, in particular to a direct electrochemical method capable of detecting fencetin and / or cerequinone in biological samples. Background technique [0002] Qinpi is a traditional Chinese herbal medicine with a long history and wide application. According to the "Chinese Pharmacopoeia" (2015 edition), the main active ingredients of chinensis are fennecine A and fencetine B, these two components are the main symbolic components for evaluating the quality of chinensis, and their structural formulas are as follows: [0003] [0004] Pharmacological experiments have shown that teretin and teretin can effectively inhibit the growth of microorganisms, and have anti-inflammatory, analgesic and anti-tumor effects. Therefore, it is of great significance to establish a method for the quantitative detection of terecetine and terecetine separately or simultaneously, and to study the...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30G01N27/327
CPCG01N27/48G01N27/308G01N27/3278
Inventor 程寒鲍昌昊施敏马雯雯方涛黄先菊李竣
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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