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Method for measuring pesticide resistance of macrosiphum avenae

A technology for the resistance of aphids and insecticides, which is applied in the field of monitoring and measuring the resistance of agricultural pests, can solve the problems of no improvement, cumbersome operation, and shorten the time for checking results, and achieve intuitive and clear results, easy to use, and overcome sample large number of effects

Inactive Publication Date: 2014-04-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Toxicity determination is a common method for indoor understanding of insecticide resistance, but the traditional immersion method and drip method are cumbersome to perform, and several concentrations must be set and repeated to ensure the reliability of the results.
The glass tube drug film method is a new method for rapid detection of pest resistance. Due to its obvious effect, it shortens the time for checking results, but it is still the same as the traditional method in terms of concentration and repetition.

Method used

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  • Method for measuring pesticide resistance of macrosiphum avenae
  • Method for measuring pesticide resistance of macrosiphum avenae
  • Method for measuring pesticide resistance of macrosiphum avenae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Obtaining of embodiment 1 diagnostic dosage

[0025] 1. Research object

[0026] The research object of the present invention is the mixed population of the aphid aphid from various places raised in the laboratory for multiple generations.

[0027] 2. Method

[0028] The test drugs imidacloprid, pirimicarb, omethoate and beta-cypermethrin were tested for their toxicity by glass tube drug film method.

[0029] 2.1 Configuration and dilution of the mother liquor of the original drug

[0030] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C.

[0031] Before the toxicity test, the original drug mother solution was diluted to various concentrations with acetone and set aside.

[0032] 2.2 Preparation of glass tube drug film

[0033] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from th...

Embodiment 2

[0042] Example 2 Diagnosis Dosage Detection of Drug Resistance of Aphid aphid

[0043] 1. Research object

[0044] The object of this case study is the tested population of the field aphid.

[0045] 2. Method

[0046] For the test agents imidacloprid, pirimicarb, omethoate and beta-cypermethrin, the diagnostic dosage of the present invention was used to make glass tube drug films, and the resistance level of Aphid aphids was tested in the field.

[0047] 2.1 Configuration and dilution of the mother liquor of the original drug

[0048] The original drug is dissolved in acetone, and the diagnostic dose obtained by diluting the original drug mother liquor in Example 1 with acetone is set aside.

[0049] 2.2 Preparation of glass tube drug film

[0050] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the diluted drug solution and add it to a glass tube with a diameter of 1.7cm and a height ...

Embodiment 3

[0055] Example 3 Determination of drug resistance of the field tube aphid by chemical control diagnosis glass tube

[0056] 1. Research object

[0057] The object of this case study is the tested population of the field aphid.

[0058] 2. Method

[0059] The test drugs imidacloprid, pirimicarb, omethoate and beta-cypermethrin were used to make a glass tube drug film with the diagnostic dose of the present invention, and the drug resistance of the field aphid was measured.

[0060] 2.1 Operation steps

[0061] 1) Take wheat plants with aphids according to field sampling standards and bring them back indoors;

[0062] 2) Shake the wheat seedlings to make the aphids fall off on the white paper, select the lively tube aphids and put them into diagnostic glass tubes, 10-20 heads per tube (choose wingless adult aphids as much as possible), seal with gauze, 3-5 per drug Duplicate diagnostic reagent tubes;

[0063] 3) Place the diagnostic reagent tubes containing the aphids indoo...

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Abstract

The invention provides a method for measuring the pesticide resistance of macrosiphum avenae. The method comprises the following steps of manufacturing a diagnosis glass pipe under the diagnosis dosage of a pesticide, rapidly detecting the resistance level to the pesticide from macrosiphum avenae field population, wherein the pesticide is imidacloprid, pirimor, omethoate or beta-cypermethrin, and the diagnosis dosage of the pesticide comprises 4289ng / cm<2> of the imidacloprid, 103ng / cm<2> of the pirimor, 224ng / cm<2> of the omethoate, and the 4695ng / cm<2> of the beta-cypermethrin. According to the method provided by the invention, the use is simple and convenient, the result is intuitive, the method is suitable for rapidly detecting macrosiphum avenae resistance level in the field, the disadvantages that the number of samples in the traditional detecting method is large, the operation is tedious, the time is wasted and the like are overcome, and the method is used for rapidly detecting the macrosiphum avenae field resistance level.

Description

technical field [0001] The invention relates to a method for measuring the drug resistance of agricultural pests, in particular to a method for detecting the drug resistance of the aphid. Background technique [0002] Wheat aphid is one of the important agricultural pests that damage wheat worldwide. It is found in every wheat-producing country in the world and occurs in both the north and south wheat regions of my country. The aphid can not only directly suck the nutrients of plants, causing serious dehydration and malnutrition of plants, but also spread viruses, cause greater indirect damage to plants, and cause huge economic losses to agricultural production. For a long time, the control of Aphid aphid has been mainly controlled by chemical control. Due to the massive, continuous and irrational use of chemical insecticides, the Aphid aphid in many countries and regions has developed varying degrees of resistance to commonly used insecticides. drug resistance. [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 高希武辛娟娟鲁艳辉刘晓岚张帅肖达杨婷王思一宋敦伦
Owner CHINA AGRI UNIV
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