Method for detecting organophosphorus pesticide residue in tobacco

Abstract

The invention discloses a method for detecting the organophosphorus pesticide residue in the tobacco, comprising the following steps of preprocessing by adding water of 5-15 times the weight of the tobacco sample to the tobacco sample to sock the tobacco sample, adding 10-20 ml of extracting agent to each gram of the tobacco sample to extract the tobacco sample, centrifuging to obtain organic-phase supernatant, purifying the organic-phase supernatant, concentrating the organic-phase supernatant by blowing with nitrogen, adding acetone solution to the organic-phase supernatant, and filtering the organic-phase supernatant with a organic-phase filtering membrane of 0.2 Mum to obtain the sample solution, wherein the extracting agent is the mixed solution of acetonitrile and acetone which are at the volume ratio of 1-2:1; and detecting the organophosphorus pesticide residue in the sample solution with gas chromatography and mass spectroscopy by using the chlorazol phosphorus as the internal standard substance after finishing preprocessing. Because the extracting agent is used for extracting in the preprocessing step, the target substance in the tobacco sample can be effectively extracted into the organic phase, and the precision for detecting the organophosphorus pesticide residue in the tobacco can be greatly improved.

Description

technical field [0001] The invention relates to a rapid detection method for organophosphorus pesticide residues in tobacco, belonging to the technical field of trace analysis. Background technique [0002] During the planting and storage of tobacco, large pesticide residues will be produced. Among them, organophosphorus pesticides (OPs) have many varieties, wide activity and high efficacy, and are widely used. Due to the unstable chemical properties of these organophosphorus pesticides, they are relatively easy to oxidize, hydrolyze, etc., and are often oxidized into compounds that are more toxic than the original pesticides in the living body. Health hazards are enormous. Therefore, no matter from the perspective of tobacco and tobacco product safety or environmental protection, the detection of organophosphorus pesticide residues in tobacco is of great significance. At present, the research on the analysis method of pesticide residues in tobacco and tobacco products is ...

AI Technical Summary

Problems solved by technology

[0003] In the detection of pesticide residues, the treatment of tobacco samples is often the main factor affecting the detection efficiency. Due to the complexity of tobacco samples, many pre-treatment methods can only target a single or a small number of analysis objects, or the pre-treatment steps are too cumbersome. The operation is difficult, the reagent consumption is high, and the extraction efficiency is difficult to improve

Such as the current GB / T13595-2004 method for detecting organophosphorus pesticide residues in tobacco and tobacco products. More than 6 kinds of organic solvents are used; moreover, the loss or damage of the analysis object will be caused during the pretreatment process, which will affect the final test result

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