Detection method of organophosphorus pesticide residues in tea

A technology of organophosphorus pesticides and detection methods, applied in Raman scattering, material excitation analysis, etc., can solve the problems of false positives, expensive instruments, poor reliability, etc., to overcome false positives, high sensitivity, and reduce detection interference Effect

Inactive Publication Date: 2013-07-10
XIAMEN UNIV
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Problems solved by technology

At present, the detection of pesticide residues in tea mainly uses large-scale instruments such as gas chromatography, liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry. The instruments are expensive and the operators must have considerable professional skills. The detection cycle is long, which is not conducive to front-line supervision department to popularize
The rapid detection of pesticide residues in tea mainly focuses on organophosphorus pesticides, and the enzyme inhibition method is widely used. Rapid detection, but the activity of the enzyme is easily interfered by pH and other conditions such as inhibitors, resulting in false positives, and the reliability of the method is poor

Method used

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  • Detection method of organophosphorus pesticide residues in tea
  • Detection method of organophosphorus pesticide residues in tea

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Embodiment 1

[0020] The detection of methyl parathion in the tealeaves of embodiment 1

[0021] Instrument: B&W TEK iRaman; power: 40mw; integration time: 10s (A), 20s (B, C, D)

[0022] Sample to be tested:

[0023] (1) 10ppm methyl parathion standard substance, its configuration method is: transfer 1ml of 100ppm methyl parathion standard substance (solvent is acetone) into a 10ml volumetric flask, and prepare 10ppm methyl parathion standard substance with ultrapure water Phosphorus solution.

[0024] (2), blank tea leaves

[0025] (3) For tea leaves containing 2ppm methyl parathion, the preparation method is as follows: take 5g of tea leaves and put them into a beaker, then add 0.1ml of 100ppm methyl parathion standard solution (solvent is acetone) dropwise, and place in the air Let it dry naturally for later use.

[0026] (4) For tea leaves containing 1ppm methyl parathion, the preparation method is as follows: take 5g of tea leaves and put them in a beaker, then add 0.05ml of 100pp...

Embodiment 2

[0036] The detection of omethoate in embodiment 2 tealeaves

[0037] Instrument: B&W TEK iRaman; power: 40mw; integration time: 10s (A), 20s (B, C, D)

[0038] Sample to be tested:

[0039] (1), 10ppm omethoate standard substance, its preparation method is: all move 1ml100ppm omethoate standard substance (solvent is acetone) in the 10ml volumetric flask, be mixed with ultrapure water 10ppm methyl parathion solution.

[0040] (2) For tea leaves containing 1ppm omethoate, the preparation method is as follows: take 5g of tea leaves and put them into a beaker, then add 0.05ml of 100ppm omethoate standard solution (solvent is acetone) dropwise, and place them in the air to dry naturally for later use .

[0041] (3), blank tea leaves

[0042] 1. Extraction

[0043] Take 5g of tea leaves (accurate to 0.01g) and add 15ml of acetonitrile, ultrasonically extract for 10min, take out the supernatant and put it in a 25ml chicken heart bottle, blow it to nearly dry with nitrogen in a wa...

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Abstract

The invention relates to a detection method of organophosphorus pesticide residues in tea. By using the surface enhanced Raman spectroscopy (SERS) as the detection method and using nano-gold as an SERS substrate, the tea is pretreated, and organophosphorus pesticides on the tea are enriched and separated so as to obtain a surface enhanced Raman spectrogram spectrogram by using a portable Raman spectrometer. Experiments prove that the detection method of the organophosphorus pesticide residues in the tea is easy to operate and high in accuracy, and can quickly detect organophosphorus in the tea, and false positive is avoided.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and specifically relates to a surface-enhanced Raman detection method for organophosphorus pesticide residues in tea leaves. Background technique [0002] my country is a large agricultural country, and tea is one of the most culturally distinctive and economically valuable crops in my country's agricultural products. With the rapid development of the domestic economy, my country has gradually become a world-wide tea production and sales country. However, in recent years, the problem of pesticide residues in tea has become increasingly serious, and has received widespread attention from all walks of life at home and abroad. Pesticide residues in tea directly cause harm to the human body through brewing, and its potential threat and enrichment effect make it a serious social problem. The hygienic quality of tea is directly related to the health of consumers. With the improvement of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 康怀志曾勇明陈启振刘国坤田中群
Owner XIAMEN UNIV
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