Immune colloidal gold test strip for detecting organophosphorus pesticide and method for making same

Abstract

This invention discloses one immune glue gold test bar and process method for organophosphorus in agriculture test technique field, wherein, the test bar comprises PVC underlay, adhesive agent layer, sample pad, combination pad, aqua fortis fiber film, weight band, test band, compare band, overlap band, water absorptive pad, limit line and arrow line. The process method comprises the following steps: processing the medicine BSA load protein coupler; processing the medicine special signal clone antigen; processing sheep, rabbit or mouse antigen IgG; processing glue gold; processing single clone antigen gold label.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a test strip for detecting organophosphorus pesticide residues using antibody immune colloidal gold technology and a preparation method thereof. Background technique [0002] At present, the main methods for the detection of pesticide residues in my country still rely on liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography-tandem mass spectrometry (HPLC / MS / MS), gas chromatography-mass spectrometry (GC / MS). ) and other large-scale modern analytical instruments. Although these instruments have high detection sensitivity, they are expensive and require highly trained professional technicians to operate and use. At the same time, it takes several hours or even overnight from sample processing to final results. The operation process during this period requires several steps to more than ten steps, which cannot meet the timely an...

AI Technical Summary

Problems solved by technology

[0002] At present, the main methods for the detection of pesticide residues in my country still rely on liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography-tandem mass spectrometry (HPLC / MS / MS), gas chromatography-mass spectrometry (GC / MS). ) and other large-scale modern analytical instruments. Although these instruments have high detection sensitivity, they are expensive and require highly trained professional technicians to operate and use. At the same time, it takes several hours or even overnight from sample processing to final results. The operation process during this period requires several steps to more than ten steps, which cannot meet the timely and rapid detection of large-scale samples or temporary samples technically and economically, so that people's requirements for food quality and safety in daily life cannot be guaranteed. Therefore, research on vegetable And the rapid detection technology of pesticide residues in food has become a concern of the competent departments at all levels and the general residents. Therefore, sensitive, rapid and accurate detection equipment and methods for organophosphorus should be developed as soon as possible for the detection of vegetables, fruits, tissues and other samples. Organophosphorus residues are of great significance

In the existing similar technology, the manufacturer of the ELISA kit is mainly EnviroLogix Inc. of the United States. The test sample is water body, which cannot be used to detect the organophosphorus pesticide residues in fruits and vegetables, and the minimum detection limit is 0.023ppb; in addition, domestic, In addition, there are many manufacturers, such as American Neogen Company and domestic Zhejiang Houxiu Biological Products Development Co., Ltd., which develop and produce a variety of rapid test cards, which generally use the principle of cholinesterase inhibition to detect organic phosphorus in fruits and vegetables. Pesticide residues: Although this kind of quick test card applies the national standard GB / T5009.199 to quickly detect the residues of organophosphorus and carbamate pesticides in vegetables, it is not perfect for the detection of pesticide objects, such as the detection limit of chlorpyrifos. No instructions; and the above quick test card is only suitable for the processing of a small amount of samples, cut into pieces about 1cm square, take 5g into a bottle with a cap, add 10mL of buffer solution, shake 50 times, and let it stand for more than 2min to test immediately. Therefore, only the pesticide content on the surface of the sample can be detected actually.

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