An axial gradient protein-loaded silk nerve conduit and its preparation and application

Abstract

The invention relates to the field of biomedical engineering, and discloses an axial gradient protein-loaded silk nerve guide and its preparation and application. In the present invention, by grafting o-nitrobenzyl photosensitive molecules on silk fibroin, silk fibroin modified by o-nitrobenzyl photosensitive molecules is obtained, and then prepared into a photoactive silk nerve guide, and finally The silk nerve guide is soaked in the nerve growth factor solution, the front end, the middle part and the end are irradiated with blue light along the axial direction, after washing and freeze-drying, an axial gradient protein-loaded silk nerve guide is obtained. The invention gradually reduces the light irradiation time in the front, middle and end regions of the silk nerve guide, and realizes the precise and controllable gradient loading of the silk nerve guide. The biological activity of the silk nerve guide of the invention is greatly improved, can effectively promote the proliferation and migration of nerve cells, can induce the rapid growth of new nerve tissue, and achieve good nerve repair effect in clinical practice.

Description

technical field

[0001] The invention relates to the field of biomedical engineering, in particular to an axial gradient protein-loaded silk nerve guide and its preparation and application. Background technique

[0002] Rapid and effective repair of peripheral nerve injury (PNI) is one of the unsolved worldwide clinical problems. Every year, there are about 2 million new PNI patients in the world, so there is a huge clinical demand for PNI repair. However, various tissue-engineered nerve bridges (stents / catheters) currently used for PNI repair still cannot achieve the repair effect of autologous nerve transplantation, especially it is difficult to quickly and effectively repair long-distance nerve defects and induce rapid regeneration of new nerve tissue. grow. The fundamental reason is that this type of bridge lacks biological signals that can effectively promote PNI repair. Photopatterning technology provides a powerful means for introducing biochemical signals into biol...

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