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Silk protein nerve conduit and preparation method thereof

A nerve conduit, silk protein technology, applied in pharmaceutical formulations, coatings, medical science and other directions, can solve the problems of unstable silk protein solution, poor mechanical properties, etc.

Active Publication Date: 2021-01-26
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the research of our team, through the combination of silk protein weaving technology and silk protein film-forming technology, a composite catheter with good mechanical properties was successfully obtained and applied to the replacement of small blood vessels. However, due to the instability of silk protein solution However, how to stably obtain water-insoluble silk protein fiber-film composite catheter still needs technical improvement and breakthrough on the basis of understanding the mechanism of silk protein; in addition, we successfully obtained silk protein nanofibers with oriented structure through electric field-induced migration Silk protein gel or sponge, and it has been successfully applied to wound repair and induced migration of nerve cells, but the mechanical properties of the gel or sponge are poor, and it is difficult to meet the requirements of clinical applications. Therefore, this invention is based on the team's deep understanding of silk protein It is hoped that through the combination and optimization of different technologies, a composite nerve conduit with excellent mechanical properties on the outside to meet the application requirements and an oriented silk protein sponge on the inside can be prepared to provide orientation-inducing signals to obtain a better repair effect

Method used

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  • Silk protein nerve conduit and preparation method thereof
  • Silk protein nerve conduit and preparation method thereof
  • Silk protein nerve conduit and preparation method thereof

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preparation example Construction

[0036] The present invention also provides a preparation method of the above-mentioned silk protein nerve guide, comprising: S1) braiding silk protein fibers to obtain a catheter matrix; S2) soaking the catheter base in a silk protein solution to obtain a catheter part; S3) The silk protein nanofiber gel with an orientation structure is combined with the conduit part, and after freeze-drying, the silk protein nerve conduit is obtained.

[0037] The present invention has no special limitation on the sources of all raw materials, which can be commercially available.

[0038] The catheter matrix is ​​obtained by weaving silk protein fibers; the silk protein fibers are preferably silk, more preferably degummed silk, and more preferably 80-100 denier degummed silk; the braiding angle of the braiding is preferably 45°-60°; The speed of the knitting is preferably 30-60r / min, more preferably 40-50r / min, and more preferably 45r / min; the gear ratio during the knitting is preferably (60-...

Embodiment 1

[0050] (1) Select the smooth first mold with a diameter of 3mm, use a cooked silk thread of 100 denier degummed silk, and weave a skeleton structure on it through a textile machine. The weaving angle is 45°, the speed is 45r / min, and the gear ratio is 80:40 ; The woven skeleton structure is a braided structure.

[0051] (2) Submerge the braided catheter completely in 2% silk protein aqueous solution (the content of silk protein nanofibers is 0.8% of the total mass of silk protein), the volume ratio of the catheter to the silk protein aqueous solution is 1:2, at 10°C Under certain conditions, with the slow evaporation of water in the silk protein solution, a layer of insoluble silk fibroin film will be formed on the skeleton, and a silk protein fiber-silk protein film composite catheter will be obtained.

[0052] (3) Prepare 0.2% oriented silk protein nanofiber gel, the basic parameters of electric field induction are: electric field intensity 10V, treatment time 5 minutes. Th...

Embodiment 2

[0056] (1) Select the smooth first mold with a diameter of 5mm, use a cooked silk thread of 100 denier degummed silk, and weave a skeleton structure on it through a textile machine. The weaving angle is 60°, the speed is 45r / min, and the gear ratio is 80:40 ; The woven skeleton structure is a braided structure.

[0057] (2) Submerge the braided catheter completely in 10% silk protein solution (the content of silk protein nanofibers is 4% of the silk protein mass), the volume ratio of the catheter to the silk protein aqueous solution is 1:2, at 90°C Under certain conditions, with the slow evaporation of water in the silk fibroin solution, a layer of insoluble silk fibroin film will be formed on the skeleton, and a silk protein fiber-silk protein film composite catheter will be obtained.

[0058] (3) Place the catheter along a direction parallel to the electrodes and immerse it in 4% silk protein nanofiber gel, then perform electric field treatment on the gel, the electric field...

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Abstract

The invention provides a silk protein nerve conduit. The silk protein nerve conduit comprises a conduit part and filling sponge filled in the conduit part, wherein the conduit part comprises a conduitbase body and a silk protein film compounded on the surface of the conduit base body; the conduit base body is formed by weaving silk protein fibers; and the filling sponge is formed by silk proteinnanofiber gel with an orientation structure. Compared with the prior art, the silk protein nerve conduit has the advantages that the silk protein nanofiber sponge with the orientation structure is arranged in the conduit, and the orientation structure is consistent with the direction of nerve axon, so that an orientation induction signal can be provided, migration and proliferation of nerve cellsare promoted, regeneration and extension of nerve fibers can be guided, and the function of the nerve conduit is remarkably improved; and therefore, the silk protein nerve conduit is combined with thesilk protein orientation sponge, so that the problems of mechanical property and orientation induction are solved.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a silk protein nerve guide and a preparation method thereof. Background technique [0002] Peripheral nerve injury is a common clinical condition. Autografts are often used to bridge peripheral nerve defects. However, limited availability and donor site morbidity still exist. Therefore, to overcome this limitation, researchers developed biomaterials based on nerve conduits to aid peripheral nerve regeneration. Artificial conduits have demonstrated the ability to fill neural gaps, synthetic nerve conduits are expected to replace autografts, and improvements in biomaterials hold great potential for promoting nerve regeneration and functional restoration. Therefore, the developed nerve guide not only has good biological performance, but also should provide a better microenvironment for the regeneration of nerve tissue. [0003] At present, there are many n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/34A61L27/52A61L27/56A61L27/58
CPCA61L27/227A61L27/34A61L27/52A61L27/58A61L27/56A61L2430/32A61L2420/02A61L2400/12C08L89/00
Inventor 吕强路青青张锋
Owner SUZHOU UNIV
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