Neural restoration sleeve and preparing method and application thereof

A nerve repair and cannula technology, applied in the field of nerve cannula, can solve the problems of limited clinical use, difficulty in use, nerve compression, etc., and achieve the effect of accelerating nerve tissue repair, good biocompatibility, and simple method

Inactive Publication Date: 2016-03-23
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural materials that can be used as nerve sleeves include blood vessels, denatured muscle, laminin, fibronectin, collagen, etc. Natural materials can sometimes cause adverse immune reactions, although immunosuppressants can play a role, but in injury or In the case of tumors, it is not easy to use; in addition, natural proteins are difficult to mass-produce, and the quality and performance are difficult to control. People have turned more attention to artificial materials
Non-degradable artificial material sleeves include silicone sleeves and polytetrafluoroethylene sleeves. The simple use of such sleeves has no effe

Method used

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  • Neural restoration sleeve and preparing method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In this embodiment, the nerve repair sleeve is prepared by the following method, which specifically includes the following steps:

[0044] (1) Dissolve polycaprolactone in a mixture of chloroform and N,N-dimethylformamide at a volume ratio of 1:1 to prepare a solution with a mass volume concentration of 30%, and then stir at 50°C 24h to obtain a polymer solution; 10 mg of epidermal growth factor was dissolved in 1 mL of Tris-hydrochloric acid buffer at pH=7.4, and then dispersed in the polymer solution to obtain a polymer solution dispersed with nerve growth factor;

[0045] (2) The polymer solution dispersed with nerve growth factor is injected into the electrospinning equipment through a syringe (with a No. 5 stainless steel needle, diameter 1-5mm) for spinning, and the parameters during spinning are set to: spinneret voltage The solution flow rate is 10kV, and the solution flow rate is 3mL / h; the formed fibers are ejected from the spinneret of the electrospinning equ...

Embodiment 2

[0047] In this embodiment, the nerve repair sleeve is prepared by the following method, which specifically includes the following steps:

[0048] (1) Glycolide-lactide copolymer (glycolide content greater than 80%) is dissolved in a mixture of chloroform and N,N-dimethylformamide with a volume ratio of 2:1 to prepare A solution with a mass volume concentration of 10%, and then stirred at 60° C. for 20 h to obtain a polymer solution; 5 mg of epidermal growth factor was dissolved in 1 mL of Tris-hydrochloric acid buffer at pH=7.4, and then dispersed in the polymer solution to obtain A polymer solution dispersed with nerve growth factor;

[0049] (2) The polymer solution dispersed with nerve growth factor is injected into the electrospinning equipment through a syringe (with a No. 5 stainless steel needle, diameter 1-5mm) for spinning, and the parameters during spinning are set to: spinneret voltage is 15kV, and the solution flow rate is 1mL / h; the formed fibers are ejected from...

Embodiment 3

[0051] In this embodiment, the nerve repair sleeve is prepared by the following method, which specifically includes the following steps:

[0052] (1) Dissolving caprolactone-glycolide copolymer (caprolactone content greater than 70%) in a mixture of chloroform and N,N-dimethylformamide with a volume ratio of 3:1 is prepared A solution with a mass volume concentration of 1%, and then stirred at 80° C. for 12 hours to obtain a polymer solution; 15 mg of platelet-derived proliferation factor was dissolved in 1 mL of Tris-hydrochloric acid buffer at pH=7.4, and then dispersed in the polymer solution Obtaining a polymer solution dispersed with nerve growth factor;

[0053] (2) The polymer solution dispersed with nerve growth factor is injected into the electrospinning equipment through a syringe (with a No. 5 stainless steel needle, diameter 1-5mm) for spinning, and the parameters during spinning are set to: spinneret voltage is 30kV, and the solution flow rate is 1mL / h; the forme...

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Abstract

The invention provides a neural restoration sleeve and a preparing method and application thereof. The neural restoration sleeve is a double-layer fiber sleeve, the inner layer of the sleeve is an orientation polymer fiber layer, the outer layer of the sleeve is a non-orientation polymer fiber layer, bioresorbable polymers are adopted, and the nerve growth promoting factors are embedded in the inner layer. The neural restoration sleeve prepared through the preparing method can induce nerve cells to grow in the fiber orientation, meanwhile, the nerve growth promoting factors are embedded in the inner layer to promote growth of nerve axon and accelerate nerve tissue restoration, the non-woven non-orientation polymer fiber layer on the outer layer plays a role in supporting and endows the neural restoration sleeve with high strength and stability, and the polymers on the inner layer and the outer layer are the bioresorbable polymers, have high biocompatibility, will not cause chronic inflammation reaction and are high in safety; the preparing method is simple, and industrial production can be realized.

Description

technical field [0001] The invention belongs to the technical field of nerve sleeves, and relates to a nerve repair sleeve, a preparation method and application thereof. Background technique [0002] Peripheral nerve injury is a disease often encountered in clinical work, which can lead to loss of sensation and motor dysfunction. Since nerve cells do not divide, their growth is impenetrable, making regeneration in the body very difficult. At present, various intervention strategies such as tissue or cell transplantation, tissue and bioengineering, electromagnetic and other physical factors, nerve stimulation or regulation, drugs or chemicals are used to promote the regeneration, repair and reconstruction of destroyed or damaged nerves on the basis of the original nerve anatomy and function. Modeling and reconstructing neuroanatomical projection pathways and circuits, regulating and improving nerve signal conduction can all partially achieve neurological function restoration...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/58A61L27/18D04H1/76D01D1/02D01D5/00
CPCA61L27/54A61L27/18A61L27/58A61L2300/252A61L2300/414A61L2430/32D01D1/02D01D5/003D04H1/76C08L67/04
Inventor 韩志超许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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