Preparation method and application of degradable carboxymethyl chitosan composite nerve conduit

A technology of carboxymethyl chitosan and nerve conduits, which is applied in the direction of catheters, etc., can solve the problems of poor formability of water-soluble carboxymethyl chitosan, and achieve the effect of low cost and simple preparation process

Active Publication Date: 2012-07-04
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor formability of water-soluble carboxymethyl chitosan, it can be quickly dissolved after contacting water. These characteristics have become a major obstacle for carboxymethyl chitosan to be used as a scaffold material for peripheral nerve repair catheters.

Method used

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  • Preparation method and application of degradable carboxymethyl chitosan composite nerve conduit
  • Preparation method and application of degradable carboxymethyl chitosan composite nerve conduit
  • Preparation method and application of degradable carboxymethyl chitosan composite nerve conduit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Prepare the preparation of the original catheter solution:

[0026] 1) Dissolve medical carboxymethyl chitosan dry powder in water for injection, stir well, adjust pH to 9 with sodium hydroxide solution of appropriate concentration, let stand to remove air bubbles, make final carboxymethyl chitosan solution concentration be 2.5 %(m / V);

[0027] 2) Dissolving medical polyvinyl alcohol in hot water for injection, fully stirring, and standing to remove air bubbles, the final concentration of polyvinyl alcohol solution is 10% (m / V);

[0028] 3) After mixing the carboxymethyl chitosan solution and the crosslinking agent 1,4-bisglycidyl ether in a ratio of 100 / 4 (m / m), let it stand to remove air bubbles, put it in an oven, and carry out crosslinking React for 20 hours.

[0029] 4) Add the polyvinyl alcohol solution in proportion to the cross-linked carboxymethyl chitosan solution (polyvinyl alcohol / carboxymethyl chitosan=2 / 1, m / m), stir well, and let stand to remove bub...

Embodiment 2

[0037] 1. Prepare the preparation of the original catheter solution:

[0038] 1) Dissolve medical carboxymethyl chitosan dry powder in water for injection, stir well, adjust pH to 10 with sodium hydroxide solution of appropriate concentration, let stand to remove air bubbles, make final carboxymethyl chitosan solution concentration be 2.0 %(m / V);

[0039] 2) Dissolving medical polyvinyl alcohol in hot water for injection, fully stirring, and standing to remove air bubbles, the final concentration of polyvinyl alcohol solution is 10% (m / V);

[0040] 3) After mixing carboxymethyl chitosan solution, polyvinyl alcohol and 1,4-crosslinking agent bisglycidyl ether in a ratio of 100 / 4 (m / m), let it stand to remove air bubbles, and put it in an oven , to carry out the cross-linking reaction for 18 hours.

[0041] 4) Add the polyvinyl alcohol solution in proportion to the cross-linked carboxymethyl chitosan solution (polyvinyl alcohol / carboxymethyl chitosan=1 / 1, m / m), fully stir, and...

Embodiment 3

[0045] 1. Prepare the preparation of the original catheter solution:

[0046] 1) Dissolve medical carboxymethyl chitosan dry powder in water for injection, stir well, adjust pH to 10 with sodium hydroxide solution of appropriate concentration, let stand to remove air bubbles, make final carboxymethyl chitosan solution concentration be 2.0 %(m / V);

[0047] 2) Dissolving medical polyvinyl alcohol in hot water for injection, fully stirring, and standing to remove air bubbles, the final concentration of polyvinyl alcohol solution is 10% (m / V);

[0048] 3) After mixing the carboxymethyl chitosan solution and the cross-linking agent 1,4-bisglycidyl ether in a ratio of 100 / 2 (m / m), let it stand to remove air bubbles, put it in an oven, and carry out cross-linking React for 18 hours.

[0049] 4) Add the polyvinyl alcohol solution in proportion to the cross-linked carboxymethyl chitosan solution (polyvinyl alcohol / carboxymethyl chitosan=2 / 1, m / m), stir well, and let stand to remove ...

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Abstract

The invention relates to a preparation method and an application of a carboxymethyl chitosan composite nerve conduit which has high tensile strength and can be stitched and fixed. The preparation method comprises the following steps of: blending the crosslinking carboxymethyl chitosan solution and polyving akohol solution at different volume ratios; injecting the crosslinking fluid into a mould; performing repeated freeze thawing, and performing air drying and curing; washing with water for injection; removing the solvent; and performing air drying. According to the electrophysiological and histological observation results of a peripheral nerve defect animal model, the prepared nerve conduit can realize bridge connection of the nerve-defect broken ends, has high tensile strength and long in-vivo hold time, and can promote nerve regeneration; and the nerve axon can grow along the conduit. The preparation method provided by the invention has the characteristics of simple technology and low cost, can replace the traditional nerve sewing method, and has a relatively good application prospect in clinical treatment of peripheral nerve defect.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, and relates to a preparation method of a nerve guide composed of a novel material, which can be used as a nerve bridge when treating peripheral nerve defects and can be biodegraded. Background technique [0002] The repair of peripheral nerve defects has always been a clinical problem. The excellent and good rate of traditional repair methods such as adventitial suture is only 50%. In the field of trauma surgery, nerve damage is repaired by nerve end-to-end suture. However, because the peripheral nerve is a mixed nerve, there is still a lack of fast and effective surgery. The scientific identification method to accurately distinguish the sensory nerve and motor nerve fibers at the nerve stump, even if the perineural suture is performed with fine microsurgical techniques, it is difficult to avoid the wrong butt joint of the sensory and motor nerve fibers after the end-to-end suture of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L29/06A61L29/04
Inventor 蒋丽霞魏长征奚宏伟王文斌
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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