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Heterologous decellularized nerve graft with tissue adhesion preventing function and preparation method thereof

A nerve transplantation and tissue adhesion technology, applied in tissue regeneration, coating, medical science, etc., can solve problems such as unfavorable nerve connection, scar formation, affecting surgical results, etc., and achieves low cost, tissue adhesion prevention, simple and flexible operation. Effect

Inactive Publication Date: 2017-05-31
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the above-mentioned acellular nerve graft is implanted in the body, although it can repair the nerve defect, at the same time, it will definitely promote the proliferation of fibroblasts, secrete a large amount of collagen fibers, and form scars and adhesions, which is not conducive to the connection between nerves and affects Surgical effects, sometimes leading to serious postoperative complications

Method used

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  • Heterologous decellularized nerve graft with tissue adhesion preventing function and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. At room temperature, add 2 g of sodium hyaluronate (with a weight average molecular weight of 300,000) into 100 mL of purified water, and stir magnetically for 8 hours until it is completely dissolved to obtain an aqueous solution of sodium hyaluronate with a mass fraction of 2%.

[0040] 2. Immerse the xenogeneic acellular nerve graft as a whole in the above-mentioned sodium hyaluronate aqueous solution for 10 minutes, so that the surface and interior of the acellular nerve are fully infiltrated with the solution.

[0041] 3. The xenogeneic acellular nerve graft impregnated with sodium hyaluronate solution was taken out, pre-frozen at -10°C for 6 hours, and then freeze-dried at -20°C for 10 hours.

[0042] 4. The freeze-dried product is sterilized by cobalt-60 irradiation (irradiation dose is 13KGy), and aseptically packaged to obtain an anti-adhesion xenogeneic acellular nerve graft product containing sodium hyaluronate. The schematic diagram is as follows figure 1...

Embodiment 2

[0045]1. At room temperature, add 1 g of carboxymethyl chitosan (with a weight average molecular weight of 700,000) into 100 mL of purified water, stir magnetically for 4 hours until it dissolves completely, and obtain an aqueous solution of carboxymethyl chitosan with a mass fraction of 1%.

[0046] 2. Immerse the xenogeneic decellularized nerve graft as a whole in the above carboxymethyl chitosan aqueous solution for 20 minutes, so that the surface and interior of the decellularized nerve are fully infiltrated with the solution.

[0047] 3. The heterogeneous decellularized nerve graft impregnated with carboxymethyl chitosan solution was taken out, pre-frozen at -30°C for 3 hours, and then freeze-dried at -15°C for 6 hours.

[0048] 4. The freeze-dried product is sterilized with ethylene oxide, and aseptically packaged to obtain an anti-adhesion heterogeneous acellular nerve graft containing carboxymethyl chitosan, the schematic diagram of which is shown in figure 1 shown.

...

Embodiment 3

[0051] 1. At room temperature, add 1.5 g of carboxymethyl cellulose (with a weight average molecular weight of 500,000) into 100 mL of purified water, and magnetically stir for 6 hours until it is completely dissolved to obtain a carboxymethyl cellulose aqueous solution with a mass fraction of 1.5%.

[0052] 2. Immerse the xenogeneic decellularized nerve graft as a whole in the above carboxymethyl cellulose aqueous solution for 15 minutes, so that the surface and interior of the decellularized nerve are fully infiltrated with the solution.

[0053] 3. The xenogeneic acellular nerve graft impregnated with carboxymethyl cellulose solution was taken out, pre-frozen at -20°C for 4 hours, and then freeze-dried at -10°C for 6 hours.

[0054] 4. The freeze-dried product is sterilized by electron beam irradiation (irradiation dose is 15KGy), and aseptically packaged to obtain an anti-adhesion heterogeneous decellularized nerve graft product containing carboxymethyl cellulose. The schem...

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Abstract

The invention discloses a heterologous decellularized nerve graft with a tissue adhesion preventing function and a preparation method thereof. The heterologous decellularized nerve graft capable of preventing tissue adhesion consists of a heterologous decellularized nerve graft and an anti-adhesion macromolecular layer attached to the surface of the heterologous decellularized nerve graft; the heterologous decellularized nerve graft is an extracellular matrix which is obtained through subjecting in-vitro peripheral nerves of a healthy mammal to decellularizing. The anti-adhesion heterologous decellularized nerve graft disclosed by the invention is wide in source, low in price, good in biocompatibility and safe in use and meanwhile is free of immunogenicity; a microstructure of the extracellular matrix of the peripheral nerves is completely reserved, and thus, a natural piping support and a nutrition environment are provided for regenerating nerve axons and restoring a nerve conduction function; the anti-adhesion macromolecular layer of the heterologous decellularized nerve graft capable of preventing tissue adhesion, disclosed by the invention, can be changed into gel coats which are attached to surfaces of the decellularized nerves after the heterologous decellularized nerve graft is grafted in vivo, so that the action of preventing heterologous decellularized nerves from being adhered to periphery tissue can be excellently exerted.

Description

technical field [0001] The invention relates to a heterogeneous decellularized nerve graft with the function of preventing tissue adhesion and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Peripheral nerve injury may cause partial or total loss of body sensory and motor functions, which seriously affects the functioning of the body and the quality of life of the patient. The repair of peripheral nerve defects, especially the repair of long-distance defects, has always been a difficult clinical problem. At present, the most commonly used method is to use autologous nerve transplantation to bridge the stump to repair the injury. Although the repair effect is good, there are shortcomings such as limited sources, secondary trauma and easy formation of donor site neuromas, which greatly limit its clinical application. . Nerve conduit repair materials developed in recent years have certain effects in repairing periphe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/34A61L27/56A61L27/58A61L27/50
CPCA61L27/3633A61L27/34A61L27/3675A61L27/3695A61L27/50A61L27/56A61L27/58A61L2430/32C08L5/08C08L1/286C08L3/08
Inventor 窦源东陈振华孙先昌尹晓彤任志伟
Owner YANTAI ZHENGHAI BIO TECH
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