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Double-layer nerve conduit and preparation method

A nerve conduit, double-layer technology, applied in the field of nerve conduits, can solve problems such as single function, achieve the effects of preventing scars, promoting rapid and accurate repair, and satisfying suturability

Inactive Publication Date: 2020-04-21
SUN YAT SEN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, the introduction of biologically active components is crucial to promote nerve regeneration. In the prior art, natural polymers such as collagen or conductive substances are often introduced, but these substances have a single function

Method used

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  • Double-layer nerve conduit and preparation method

Examples

Experimental program
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Effect test

preparation example Construction

[0036] The preparation method of a kind of double-layer nerve conduit of the present invention comprises:

[0037] (1) Preparation of decellularized matrix solution

[0038] Take a certain amount of decellularized matrix powder, add it to an organic solvent with a mass concentration of 1% to 15% w / v, stir for 4 to 6 days, and then place it in a ball mill at a temperature of -10 to 10°C with a power of 25 to 75Hz for ball milling 5 to 10 minutes, a total of 2 times. The milled solution was transferred to a centrifuge tube, placed in an ultra-high speed centrifuge and placed in a centrifuge with an experimental condition of 5,000-10,000 rpm for ultra-centrifugation for 3-10 minutes, and the upper layer solution was absorbed for later use.

[0039] The sources of decellularized matrix include different tissues such as brain, spinal cord, nerve, skin, small intestinal mucosal sublayer, fat, etc. of mammals such as humans, pigs, and cattle.

[0040] Organic solvents include 2,2,2...

Embodiment example 1

[0060] Example 1 Preparation of a double-layered nerve conduit containing porcine-derived decellularized neural matrix (pDNM)

[0061] pDNM was dissolved in hexafluoroisopropanol at a concentration of 5% w / v, and PCL was dissolved in 2,2,2-trifluoroethanol at a concentration of 15% w / v. The above-mentioned steps 3-5 are used for coaxial electrospinning to obtain the inner layer of the nerve guide composed of the oriented fiber membrane. Then P(LA-TMC) 70 / 30 was dissolved in 2,2,2-trifluoroethanol to make a solution of nerve guide outer layer material with a concentration of 15% w / v, and prepared according to the above steps 7-9. Double-layer nerve guide.

[0062] Its scanning electron microscope picture is shown in image 3 , has a double-layer structure, where Figure 4 The outer layer shown is a structure of randomly arranged fibers stacked, Figure 5 The inner layer shown is a core-shell structure oriented fiber, the core is PCL, and the shell is pDNM.

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Abstract

The invention provides a preparation method of a double-layer nerve conduit. Due to the design of a double-layer structure, the unification of mechanical property requirements and biological functionrequirements on the nerve conduit is achieved; acellular matrixes uniformly distributed on an inner layer can endow each fiber with biological functionality, and an oriented fiber structure can guideoriented rapid growth of nerve axons and oriented rapid migration of Schwann cells, so rapid and accurate repair of nerves is promoted; and a compact porous structure on the outer layer not only allows sufficient exchange of substances, but also can play a barrier role and prevent formation of scars. Since the double-layer nerve conduit has high mechanical properties, the double-layer nerve conduit can meet the requirement for the suturing property of a graft in the in-vivo transplantation process, and provides necessary support for nerve regeneration space.

Description

technical field [0001] The invention relates to the technical field of nerve conduits, in particular to a double-layer nerve conduit and a preparation method thereof. Background technique [0002] Nerve conduits are often used in the repair of peripheral nerve defects, which are usually hollow tubular structures that can bridge the proximal and distal ends of damaged nerves and provide a suitable microenvironment for nerve regeneration. An excellent nerve conduit needs to: 1. Promote the rapid regeneration of nerves; 2. Effectively block the invasion of external fibroblasts and inflammatory cells, etc., and maintain the space for nerve growth; 3. Fully realize the exchange of internal and external substances. The nerve conduits currently on the market are usually single-structure conduits composed of a single material (such as polyglycolic acid, collagen, etc.). Although they have achieved good results in clinical applications, they all have fatal defects, such as rapid degr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/36A61L27/50A61L27/56
CPCA61L27/18A61L27/3633A61L27/50A61L27/56A61L2430/32C08L67/04
Inventor 全大萍邓荣力陈诗浩白莹周晶杨习锋曾晨光朱庆棠刘小林
Owner SUN YAT SEN UNIV
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