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Method for preparing artificial nerve conduit based on 3D printing and artificial nerve conduit

An artificial nerve and 3D printing technology, applied in the field of artificial nerve conduits, can solve the problems of restricting clinical application of autologous nerve transplantation, and achieve the effect of facilitating cell proliferation and growth, long degradation cycle, and fast degradation speed

Inactive Publication Date: 2016-12-14
青岛三缔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] This application provides a method for preparing an artificial nerve guide based on 3D printing and an artificial nerve guide, which solves the technical problem of limiting clinical application of existing autologous nerve transplantation

Method used

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  • Method for preparing artificial nerve conduit based on 3D printing and artificial nerve conduit
  • Method for preparing artificial nerve conduit based on 3D printing and artificial nerve conduit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Preparation of silk fibroin solution: In order to remove the sericin in silk fibroin, place silk fibroin in 4.24g / L sodium carbonate solution, boil it for 20 minutes, wash it with deionized water and dry it . Dissolve silk fibroin with a 9.3mol / L lithium bromide aqueous solution, dialyze the resulting solution, remove the lithium bromide in the solution by ion exchange, and obtain fresh regenerated silk fibroin aqueous solution after centrifugation. Place the solution in an oven and slowly concentrate to the silk fibroin solution The concentration is 15%. Store at 4°C.

[0054] 2. Preparation of silk fibroin microsphere suspension: mix the above silk fibroin solution with 10% PEG solution, add emulsifier Tween-20 dropwise while stirring, and the volume ratio is 2:1, then mix The solution was placed in the refrigerator to freeze for 48 hours, and after taking it out, it was melted at room temperature to obtain a milky white solution. The solution was centrifuged at a s...

Embodiment 2

[0062] 1. Preparation of silk fibroin solution: In order to remove the sericin in silk fibroin, place silk fibroin in 4.24g / L sodium carbonate solution, boil it for 30 minutes, wash it with deionized water and dry it . Dissolve silk fibroin with a 9.3mol / L lithium bromide aqueous solution, dialyze the resulting solution, remove the lithium bromide in the solution by ion exchange, and obtain fresh regenerated silk fibroin aqueous solution after centrifugation. Place the solution in an oven and slowly concentrate to the silk fibroin solution The concentration is 20%. Store at 4°C.

[0063] 2. Preparation of silk fibroin microsphere suspension: mix the above silk fibroin solution with 10% PEG solution, add emulsifier Tween-40 dropwise while stirring, and the volume ratio is 2:1, then mix The solution was placed in the refrigerator to freeze for 48 hours, and after taking it out, it melted at room temperature to obtain a milky white solution. The solution was centrifuged at a speed...

Embodiment 3

[0071] 1. Preparation of silk fibroin solution: In order to remove the sericin in silk fibroin, place silk fibroin in 4.24g / L sodium carbonate solution, boil it for 50 minutes, wash it with deionized water and dry it . Dissolve silk fibroin with a 9.3mol / L lithium bromide aqueous solution, dialyze the resulting solution, remove the lithium bromide in the solution by ion exchange, and obtain fresh regenerated silk fibroin aqueous solution after centrifugation. Place the solution in an oven and slowly concentrate to the silk fibroin solution The concentration is 25%. Store at 4°C.

[0072] 2. Preparation of silk fibroin microsphere suspension: mix the above silk fibroin solution with 20% PEG solution, add emulsifier Tween-60 dropwise while stirring, the volume ratio is 2:1, and then mix The solution was placed in the refrigerator to freeze for 48 hours, and after taking it out, it melted at room temperature to obtain a milky white solution. The solution was centrifuged at a speed...

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Abstract

The invention discloses a method for preparing an artificial nerve conduit based on 3D printing and the artificial nerve conduit. The problem that clinical application is limited by autogenous nerve grafting is solved. A silk fibroin solution is prepared from fibroin; a silk fibroin microsphere suspension is prepared; growth factors are loaded in silk fibroin microspheres; silk biological ink containing nerve cells is prepared; the silk fibroin microspheres loaded with the growth factors and PLGA are used for preparing a composite; the silk biological ink is filled in a first printing nozzle of a 3D printer, and the composite is filled in a second printing nozzle; the second printing nozzle is controlled to print an outer tube of the artificial nerve conduit, then the first printing nozzle is controlled to print a plurality of inner tubes of the artificial nerve conduit, and finally cell culture differentiation is conducted to form the nerve conduit. The nerve conduit prepared through three-dimensional biological printing provides a base body for adhesion growth of nerve cells, provides guidance for nerve axon combination, can promote nerve regeneration and replaces autogenous nerve grafting.

Description

Technical field [0001] The invention belongs to the technical field of medical devices, and specifically relates to a method for preparing an artificial nerve catheter based on 3D printing and an artificial nerve catheter. Background technique [0002] Peripheral nerve injury is a common clinical cause of severe limb trauma. [0003] According to the "Science Times" (2004) report, in the world's new trauma cases every year, peripheral nerve injury cases account for about 1.5% to 4.0%. There are 600,000 to 900,000 new cases of peripheral nerve injury in my country each year, of which about 300,000 to 450,000 cases require nerve transplantation to repair nerves. The repair of peripheral nerve defects, especially the repair of long-distance defects, has always been a difficult clinical problem. [0004] The most commonly used method at present is to use autologous secondary nerve transplantation, allogeneic nerve transplantation, xenogeneic nerve transplantation, etc. to bridge and re...

Claims

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Application Information

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IPC IPC(8): A61L27/58A61L27/22A61L27/54B33Y10/00B33Y80/00
CPCA61L27/58A61L27/227A61L27/54A61L2300/414A61L2430/32B33Y10/00B33Y80/00C08L89/00
Inventor 王红韩芳芳王克响
Owner 青岛三缔生物科技有限公司
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