Cosmetic composition comprising culture solution of mesenchymal stem cells cultured in hpl-containing medium

A technology of platelet lysate and cosmetic composition, which is applied in the direction of medical preparations containing active ingredients, cosmetics, drug combinations, etc., can solve problems such as unpleasant smell, cosmetic discoloration, and limited use, so as to improve skin elasticity and improve wrinkles Effect

Pending Publication Date: 2021-11-30
SCM LIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, hydroquinone has been used as the most effective ingredient for pigmentation for decades, but it has been reported that long-term use can cause allergic reactions and other side effects (white spots, dark spots), and its use has been restricted in many countries. the trend of
Thiol compounds such as glutathione and cysteine ​​are difficult to use due to their unique odor and problems of not being absorbed through the skin
In addition, their glycosides and derivatives have high polarity, making it difficult to use them as ingredients in cosmetics
[0005] Moreover, ascorbic acid, which exhibits a whitening effect by inhibiting the enzymatic activity of tyrosinase, is easily oxidized, causing problems such as discoloration and odor of the cosmetics matched with it. In order to overcome its instability, ascorbyl palmitate ( Ascorbylpalmitate), Ascorbyl-dipalmitate, Ascorbyl stearate, Ascorbyl magnesium phosphate and other derivatives, but it is difficult to penetrate into the skin without using special technology , and has the disadvantage of low inhibitory activity to tyrosinase

Method used

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  • Cosmetic composition comprising culture solution of mesenchymal stem cells cultured in hpl-containing medium
  • Cosmetic composition comprising culture solution of mesenchymal stem cells cultured in hpl-containing medium
  • Cosmetic composition comprising culture solution of mesenchymal stem cells cultured in hpl-containing medium

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preparation example Construction

[0034]The present invention provides a preparation method of mesenchymal stem cell culture fluid rich in growth factors and cytokines, comprising: step 1), culturing mesenchymal stem cells in a culture medium containing human platelet lysate; step 2), obtaining cultured The stem cells are then cultured in a serum-free medium; and in step 3), the culture fluid derived from the mesenchymal stem cells is collected and filtered to obtain the stem cell culture fluid.

[0035] The "human platelet lysate" in the present invention is a turbid light yellow liquid obtained by freezing / thawing human platelets. Platelet freezing / thawing releases the large amounts of growth factors needed for cell expansion by lysing the platelets.

[0036] In the present invention, "stem cell culture fluid" refers to a substance containing components contained in a medium obtained by culturing stem cells, and the type of mesenchymal stem cells used to prepare the culture fluid is not limited. Stem cells ...

Embodiment 1

[0070] Example 1. Cultivating mesenchymal stem cells and collecting culture fluid in a medium comprising fetal bovine serum or human platelet lysate

[0071] 1.1 Obtaining bone marrow-derived monoclonal mesenchymal stem cells isolated by layer separation culture method

[0072] After the buttocks of a 35-year-old male bone marrow donor were anesthetized with a local anesthetic, the bone marrow was collected by inserting a needle into the iliac crest. In a 100 mm culture container, 10 ml of Duchenne's modified Eagle's medium (GIBCO-BRL, Life-technologies company, Minnesota, U.S.) containing 20% ​​fetal bovine serum and 1% penicillin / streptomycin were put into a culture vessel. Put 3ml of human bone marrow at 37°C, 5% CO 2 Incubate for 2 hours in a cell culture incubator. After culturing, slightly tilt one end of the culture container, and move only the upper layer of the culture container to a new container as much as possible without detaching the cells attached to the botto...

Embodiment 2

[0076] Example 2. Analysis of Growth Factors Present in FBS-CM or hPL-CM by Protein Array Method

[0077] Cytokines present in FBS-CM and hPL-CM prepared by the same method as in Example 1 were analyzed. The analysis method is to use the analysis kit (Proteome Analyzer Human XL Cytokine Array Kit, R&D Company) that can be purchased through commercial channels to implement according to the instructions provided with the kit, and the observation after color development is performed using LAS4000 ( Fuji Corporation, Japan).

[0078] A serum-free medium cultured under the same conditions without cells was used as a control group, and FBS-CM and hPL-CM prepared by the same method as in Example 1 were used as an experimental group for comparison. The array map (ARRAY MAP) that can confirm the components contained in each stem cell culture medium is as follows: figure 1 and shown in Table 1. The relative content of each growth factor was confirmed by comparing and analyzing the sp...

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Abstract

The present invention relates to: a method for preparing a stem cell culture solution which contains growth factors and cytokines and is obtained by culturing, in a serum-free medium, mesenchymal stem cells cultured in a medium containing human platelet lysates (hPLs); and a cosmetic composition using the stem cell culture solution. When the method for preparing a mesenchymal stem cell culture solution obtained from mesenchymal stem cells cultured in an hPL-containing medium is used, it is possible to obtain a mesenchymal stem cell culture solution which has a higher content of cytokines and growth factors than that of a mesenchymal stem cell culture solution obtained from mesenchymal stem cells cultured in an FBS-containing medium, and has excellent efficacy in the promotion of human dermal fibroblast proliferation, collagen synthesis, elastin synthesis, inhibition of melanin synthesis, and hair growth. The mesenchymal stem cell culture solution can be used to produce cosmetics having effects of skin regeneration, wrinkle improvement, skin elasticity enhancement, skin whitening, and hair growth, and thus can be widely used in the cosmetic field and the pharmaceutical industry.

Description

technical field [0001] The present invention relates to a method for preparing a stem cell culture solution containing growth factors and cytokines and a cosmetic composition using the stem cell culture solution. ) The mesenchymal stem cells cultured in the culture medium were obtained by culturing in serum-free medium. Background technique [0002] Skin aging is roughly divided into two types to study, one of which is "intrinsic aging", which is the aging phenomenon that increases with age, and the other is "exogenous aging", which refers to the aging caused by the external environment, that is, ultraviolet rays. Or aging caused by environmental pollution. Regarding skin aging, various theories have been proposed so far. Among them, the theory that is scientifically closest to skin aging is the theory that skin aging is caused by oxidation. Human skin is composed of the epidermis including the stratum corneum, the dermis, and the junctional tissue, wherein the stratum cor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K8/98A61Q19/08A61Q19/02A61Q7/00
CPCA61Q19/02A61Q7/00A61Q19/08C12N5/0663C12N2503/06C12N2523/00C12N2500/90C12N2500/84A61K8/99A61K8/981C12N2500/80A61P17/18A61K35/28
Inventor 宋淳旭金时那李灿周
Owner SCM LIFESCI CO LTD
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