NGF (Nerve Growth Factor) injectable in-situ hydrogel as well as preparation and application thereof

Abstract

The invention relates to NGF (Nerve Growth Factor) injectable in-situ hydrogel as well as preparation and application thereof. The NGF injectable in-situ hydrogel is obtained by adding EDC(1-ethyl amide crosslinking agent-3-(3-dimethyl amino propyl) carbide imine) and NHS (N-hydroxysuccinimide) into an aqueous solution system of hyaluronic acid and chitosan hydrochloride, and adding an NGF after HA-NHS-active ester intermediate by the hyaluronic acid under the action of the EDC/NHS under an acidic condition. The hydrogel has a pH sensitive feature; the conversion process of solution-gel can be completed under physiological conditions of a human body; the NGF injectable in-situ hydrogel can be subjected to in-situ curing under the action of pH in an acceptor at a nerve damage part after the NGF injectable in-situ hydrogel is injected into nerve tissue; the traumatic property of a surgery is avoided. The NGF can be released slowly in vitro by utilizing the environmentally sensitive feature of the hydrogel; the problems of low activity, burst release and the like caused by the facts that a half-life period is short and the diffusion and the degradation are too quick of the NGF are effectively solved; high bioactivity of the NGF is kept; the extension and the myelination of a nerve axon are promoted; therefore, the purpose of repairing nerves is achieved.

Description

technical field [0001] The invention belongs to the fields of nerve tissue engineering and regenerative medicine, and in particular relates to an injectable in situ hydrogel of nerve growth factor, its preparation and its application in nerve repair. Background technique [0002] Peripheral nerve injury is a common clinical disease. At present, nerve anastomosis, autologous nerve transplantation, allogeneic nerve transplantation, autologous non-nerve tissue transplantation, biomaterial replacement and other methods are generally used in clinical nerve repair. Among them, the processing of biomaterials into tubular structures of nerve conduits is the focus of current research. However, when encountering long-distance and thick nerve defects, such catheters made of biological materials lack the support of microenvironmental substances such as bioactive substances, and the repair effect is very limited. [0003] Nerve growth factor (NGF) is a nerve cell growth regulator with ...

AI Technical Summary

Problems solved by technology

Afterwards, the environment-sensitive properties of the hydrogel can be used to realize the slow release of NGF in the body, effectively solving the problems of NGF activity reduction and burst release c

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