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Double-nerve-factor connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and preparing thereof

A nerve factor, composite silk technology, applied in filament/thread forming, medical science, non-woven fabrics, etc., to achieve the effect of simple and easy method, improved biocompatibility and nerve repair, and large number of connections

Inactive Publication Date: 2016-11-09
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is no report on the connection of NGF and NT-3 two nerve factors in parallel conductive polymer silk membrane chemical activation method to obtain longer nerve axon growth

Method used

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  • Double-nerve-factor connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and preparing thereof
  • Double-nerve-factor connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and preparing thereof
  • Double-nerve-factor connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and preparing thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Dissolve 0.3 g of polylactic acid with a viscosity of ~2.3dL in 3.6 mL of dichloromethane, then add 0.4 mL of N,N dimethylformamide into the dissolved polylactic acid solution, sonicate for 15 minutes and continue magnetic stirring for 12 hours Make it fully miscible. Put the dissolved polylactic acid solution into the syringe pump, set the pump speed to 0.5-2.0 mL / h, the spinning voltage to 10-15 kV, the spinning distance to 8-25 cm, and the diameter of the collecting drum to be 12 cm. cm, the drum speed is 400-2000 r / min, and the electrospinning time is 3 hours, the parallel porous polylactic acid nanofilament membrane can be obtained. Prepare a mixed solution containing DBS and polyglutamic acid at a concentration of 14 mM and 14 mM, respectively, and then add 100 mL of pyrrole monomer under magnetic stirring, continue to stir and ultrasonically form a suspension, and immerse the silk film in the C pre-cooled pyrrole solution for 0.5 hours, and then slowly added dro...

Embodiment 2

[0021]Mix 3.6 mL of dichloromethane and 0.4 mL of N, N dimethylformamide evenly, then dissolve 0.3 g of polylactic acid with a viscosity of ~2.3dL in the mixture, ultrasonicate for half an hour and stir for 12 hours, then add the solution to For the syringe pump, set the corresponding parameters: pump propulsion rate is 0.5 ~ 2.0 mL / h, voltage is 10 ~ 15kV, spinning distance is controlled at 8 ~ 25 cm, collection drum diameter is 12 cm, drum speed is 400 ~ 2000 r / min, electrospinning for 3 hours. The silk film was soaked in solutions containing DBS, polyglutamic acid and pyrrole monomer concentrations of 14 mM, 28 mM and 100 mL respectively, and kept the mixed microemulsion and ferric chloride solution at 4 °C in precooling , the ferric chloride solution was added dropwise into the microemulsion and shaken for 12 hours. Activate the conductive filament membrane with a mixed solution with a pH of 5.0, EDC and NHS concentrations of 0.2 M and 0.05 M, respectively, for 0.5 hours,...

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Abstract

The invention relates to a two-nerve-factor NGF and NT-3 connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and a preparing method thereof. Firstly, a parallel porous polylactic acid filament membrane with the diameter ranging from 50 nm to 2000 nm is put into a mixed aqueous solution containing sodium alkyl sulfonate, polyglutamic acid, FeCl3 and pyrrole, then a sufficient reaction is carried out for 2-24 h with an in-situ polymerization method, the surface of the parallel filament membrane is evenly coated with polypyrrole nano-particles, washing and drying are carried out, and then conductive polypyrrole wrapped polylactic acid fiber filament membrane with the diameter ranging from 60 nm to 2500 nm is obtained; then, protein of the two nerve factors, namely, NGF and NT-3, is connected to the parallel conductive porous composite filament membrane in sequence with the carbodiimide technology, and the conducive composite filament membrane which can release nerve factors and promote nerve axon elongation and peripheral nerve repair under electrical field stimulation is obtained. The two-nerve-factor NGF and NT-3 connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and the preparing method thereof have the advantages that implementation is easy, the nerve factor connection efficiency is high, the material conductivity is high, and the nerve repair time is short.

Description

technical field [0001] The invention relates to a polypyrrole-polylactic acid parallel conductive porous composite silk membrane connected by double nerve factors and its preparation. Background technique [0002] Compared with ordinary nanofiber preparation technology, the birth of electrospinning technology provides a new idea for the preparation of nanofibers. It can prepare nanowires with smaller diameters and longer fibers and improve their biodegradability. been applied to many fields. Compared with random electrospun fibers, parallel fibers have attracted much attention due to their anisotropy, such as their high tensile strength in the longitudinal direction, and their good parallel structure can guide the growth of cells and tissues. The polypyrrole in the conductive polymer has good biocompatibility, high conductivity and good mechanical matching of the tissue, and is a good bioelectric stimulation medium. However, the brittleness, refractory degradation and unfa...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/54C08G73/06D04H1/728D01D5/00
CPCA61L27/56A61L27/18A61L27/50A61L27/54A61L2300/414A61L2430/32A61L2430/40C08G73/0611D01D5/0038D01D5/0069D01D5/0076D01D5/0092D04H1/728C08L67/04C08L77/04C08L79/04
Inventor 黄忠兵周星星尹光福廖晓明蒲曦鸣
Owner SICHUAN UNIV
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