Composite stem cell bionic nerve conduit, and preparation method thereof
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A nerve conduit and stem cell technology, applied in pharmaceutical formulations, tissue regeneration, braids, etc., can solve the problems of poor hydrophilicity of polymer materials, unsatisfactory effects, weak cell adsorption, etc. The effect of improving hydrophilicity and cell affinity, increasing surface area
Active Publication Date: 2016-11-30
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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Problems solved by technology
However, polymer materials have poor hydrophilicity and weak cell adsorption, and the effect is often not ideal when making cell-scaffold composites.
If a pore-forming agent is added during the preparation process, the residue has potential cytotoxicity and the danger of affecting nerve growth; the pore-forming method using the preparation condition control method has the disadvantages of discontinuous pores and lack of directionality. The goal of artificial nerve guides
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Embodiment 1
[0028] 1) Preparation of braided wire: make monofilaments with a diameter of 12 μm, 8 monofilaments are made into multifilaments, 2 strands of multifilaments are made into long filaments, and 2 strands of filaments with an average fineness of 6tex are heated at a temperature of 25°C and a relative humidity of 70°C. Under atmospheric conditions of %, 2 strands of filaments are twisted into a braided wire at 200 twists / m;
[0029] 2) Fabrication of the braided tube: the braided wire is braided on a 10-spindle vertical spindle braiding machine at a temperature of 20°C and a relative humidity of 65%. The braiding angle is 45°, and the inner diameter of the casing is 3mm, the wall thickness is 0.5mm;
[0030] 3) Atmospheric pressure plasma treatment: the gas used is helium, the gas temperature is 90°C, the flow rate is 6L / min, and the treatment time is 8min;
[0031] 4) Bone marrow stromal stem cells obtained from autologous or allogeneic mammals, and placed at 37°C, 5% CO 2 , Am...
Embodiment 2
[0035] 1) Preparation of braided wire: make monofilaments with a diameter of 14 μm, 6 monofilaments are made into multifilaments, 2 strands of multifilaments are made into long filaments, and 3 strands of filaments with an average fineness of 8tex are heated at a temperature of 25°C and a relative humidity of 70°C. % under atmospheric conditions, twist 2 strands of filaments into a braided wire at 250 twists / m;
[0036] 2) Fabrication of the braided tube: Put the braided wire on a 12-spindle vertical spindle braiding machine at a temperature of 20°C and a relative humidity of 65%, using a two-dimensional and three-axial braiding process with a braiding angle of 55° and an inner diameter of the casing of 4mm , the wall thickness is 0.6mm;
[0037] 3) Atmospheric pressure plasma treatment: the gas used is helium, the gas temperature is 100°C, the flow rate is 10L / min, and the treatment time is 6min;
[0038] 4) Embryonic stem cells obtained from autologous or allogeneic mammals...
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Abstract
The invention relates to a composite stem cell bionic nerve conduit. The composite stem cell bionic nerve conduit comprises a braided hose side wall; a round hose is formed via coiling of the braided hose side wall; and gel and stem cells are arranged in the round hose formed via coiling of the braided hose side wall. The materials of the composite stem cell bionic nerve conduit are high-molecular degradable materials, are easily available, and possesses certain strength and excellent biocompatibility; the nerve conduit side wall formed via braiding possesses a lot of pores with the same directivity; the compactness of the nerve conduit side wall can be adjusted via different braiding methods, so that it is beneficial for nutrient delivery and exchanging; atmospheric pressure plasma jet processing is capable of increasing nerve conduit braided fiber surface area, and improving conduit hydrophilic performance and cellular affinity; the gel in the nerve conduit is semi-solidified, a support interface and a material exchange interface are formed by the semi-solidified gel, so that it is beneficial for uniform distribution of stem cell in the nerve conduit, and cell fixing and differentiation, and it is also beneficial for growth of regenerated nerve axon and formation of myelin sheath, and repairing of long nerve.
Description
technical field [0001] The invention relates to a nerve conduit and a manufacturing method thereof, in particular to a bionic nerve conduit compounded with stem cells and a manufacturing method thereof. Background technique [0002] Nerve fiber defects are often caused by direct or indirect damage to the nerve fibers of patients due to trauma, extrusion, surgery and other reasons. Typical findings include movement disturbances, sensory disturbances, and autonomic dysfunction. The repair of nerve fibers after injury is related to many factors, but there are many problems such as long time and poor prognosis. Even the damage, rupture and defect of some nerve fibers cannot be repaired at all, and severe cases will lead to permanent disability. Therefore, the regeneration and functional recovery of nerve fibers after injury has always been a hot topic in the field of neuroscience. Designing nerve conduits with good biocompatibility, biodegradability and mechanical properties i...
Claims
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