Nerve repairing film and preparation method thereof

A nerve repair and animal tissue technology, applied in the field of medical biomaterials, can solve the problems of reducing the effect of nerve repair, the outflow of nerve growth factors, and hindering the growth of regenerated nerve fibers into the distal end of the nerve, so as to promote nerve regeneration and prevent adhesion.

Inactive Publication Date: 2015-07-01
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the gold standard for repairing peripheral nerve injury in clinical practice is still epineurium suture under the microscope. After simple nerve suture, inflammatory cells invade around the stump, and scar tissue forms between the anastomosis and nerve bundles, hindering the passage of regenerated nerve fibers The anastomosis grows into the distal end of the nerve; the exposure of the anastomosis and the outflow of nerve growth factors will also reduce the repair effect of the nerve

Method used

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  • Nerve repairing film and preparation method thereof
  • Nerve repairing film and preparation method thereof
  • Nerve repairing film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 A nerve repair membrane was prepared from pig dermis.

[0027] Take fresh pig dermis, remove excess fat, grind mechanically, rinse with deionized water for 3 times, then rinse with 0.2% trypsin solution for 2 hours, rinse with deionized water, and then rinse with 3% trypsin solution Sodium lauryl sulfate solution was shaken and rinsed for 3 hours, and rinsed with deionized water to obtain acellular porcine dermal matrix, which was freeze-dried, and the freeze-dried acellular dermal matrix was crushed with a high-speed homogenizer and dissolved in 0.5mol / L acetic acid solution, then pour it into a flat-bottomed mold, dry it at low temperature, then neutralize it with 0.1mol / L NaOH solution, wash it with deionized water until the pH is neutral, and dry it to get the nerve repair membrane (see figure 1 ), in order to prolong its degradation time, 254nm ultraviolet radiation was used to cross-link for 2h.

Embodiment 2

[0028] Example 2 The biocompatibility test of the nerve repair membrane prepared in Example 1 was carried out, including cytotoxicity test, hemolysis test and intradermal reaction test.

[0029] (1) Cytotoxic reaction test

[0030] Method: Prepare the test solution according to the ratio of nerve repair membrane with a surface area of ​​6cm2 plus 1mL extraction medium (MEM medium containing serum), (37±1)°C, (24±2)h, and take the test solution according to GB / T16886.5 -2003 stipulated in the test method of extract solution, and graded according to GB / T14233.2-2005.

[0031] Results: The cytotoxic reaction of nerve repair membrane was less than grade 1.

[0032] (2) Hemolysis test

[0033] Method: The test solution was prepared according to the proportion of nerve repair membrane with a surface area of ​​6 cm2 plus 1 mL of 0.9% sodium chloride solution, (37±1) °C, (24±2) h, and carried out according to the test method specified in GB / T16886.4-2003.

[0034] Results: The hemo...

Embodiment 3

[0038] Example 3 The nerve repair membrane prepared in Example 1 was used to repair the sciatic nerve injury of SD rats.

[0039] Establish the SD rat sciatic nerve injury model, after the test group is sutured end-to-end, the nerve repair film prepared in Example 1 is used to wrap the suture port ( figure 2 ), the control group was only sutured end-to-end. Anatomical observation was carried out 8 weeks after the operation. It can be seen that there is no tissue adhesion around the sciatic nerve of the SD rats in the test group, the nerve repair membrane has been completely degraded and absorbed, the suture is anastomosed well, and there is no neuroma formation ( image 3 ). In the SD rats in the control group, obvious tissue adhesion occurred around the sciatic nerve, and there was neuroma formation around the suture ( Figure 4 ).

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Abstract

The invention discloses a nerve repairing film and a preparation method thereof. The method mainly comprises the steps of treating raw materials, decellularizing, dissolving matrix, drying to form a film, neutralizing and washing and the like. According to the nerve repairing film prepared by the method, active ingredients of the extracellular matrix are maintained, the peripheral nerve suture is directly coated, so that an excellent microenvironment is provided to nerve regeneration, nerves are prevented from adhering to peripheral tissues, and nerve regeneration is promoted.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a nerve repair membrane and a preparation method thereof. Background technique [0002] Peripheral nerve injury is one of the common traumas in clinical practice. Studies have shown that after injury, effective measures should be taken to repair the continuity and integrity of the injured nerve, so that the proximal end of the injured nerve can issue regenerated axis buds and reach the distal end to reinnervate. target organ. If the patient is not repaired in time or the repair method is improper, it will cause neuroma or even disability, which will seriously affect the quality of life of the patient. How to improve the effect of nerve repair is still a hot topic. [0003] At present, the gold standard for repairing peripheral nerve injury in clinical practice is still epineurium suture under the microscope. After simple nerve suture, inflammatory cells invade arou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 高秀岩任孝敏姜红敖强王爱军
Owner 山东隽秀生物科技股份有限公司
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