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Scaffold material for nerve regeneration based on silk fibroin fibers

A silk fibroin and scaffold material technology, applied in the field of fibrous scaffolds, can solve the problems of poor electrical conductivity of silk fibroin and inability to repair nerves, achieve good biocompatibility and mechanical properties, good nerve repair, and ensure nerve repair. The effect of conduction

Inactive Publication Date: 2018-02-16
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems in the prior art, the present invention provides a scaffold material for nerve regeneration based on silk fibroin fibers, which solves the problem that silk fibroin has poor electrical conductivity and cannot be used for nerve repair, and the prepared scaffold material not only has good Biocompatibility and mechanical properties, and good electrophilicity, the conduction of electrical signals by nerve guides is not hindered

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A scaffold material for nerve regeneration based on silk fibroin fibers, characterized in that: the scaffold material is composed of silk fibroin, polyaniline and gelatin, the molecular weight of the silk fibroin is 100,000, and the weight content is 97.5%; The above-mentioned gelatin is white gelatin, the weight content is 2%, and the molecular weight is 60,000; the described polyaniline molecular weight is 10,000, and the weight content is 0.5%.

[0030] The scaffold material is prepared according to the following steps:

[0031] Step 1: Put silkworm cocoons into distilled water and cook for 30 minutes, take them out and soak them in a solution of 0.2% sodium carbonate and 0.025% polyethylene glycol octylphenyl ether for 2 hours to obtain degummed silk fibers, wash with distilled water, and dry overnight at 60°C ;

[0032] Step 2: Dissolve the degummed silk fibers in a lithium bromide chaotropic solvent with a concentration higher than 9M, stir with a magnet at 60°C ...

Embodiment 2

[0047] A scaffold material for nerve regeneration based on silk fibroin fibers, characterized in that: the scaffold material is composed of silk fibroin, polyaniline and gelatin, the molecular weight of the silk fibroin is 300,000, and the weight content is 93%; The above-mentioned gelatin is white gelatin, the weight content is 5%, and the molecular weight is 80,000; the described polyaniline molecular weight is 20,000, and the weight content is 2%.

[0048] The scaffold material is prepared according to the following steps:

[0049] Step 1: Put silkworm cocoons into distilled water and cook for 30 minutes, take them out and soak them in 0.2% sodium carbonate and 0.025% polyethylene glycol octyl phenyl ether solution for 6 hours to obtain degummed silk fibers, wash with distilled water, and dry overnight at 60°C ;

[0050] Step 2: Dissolve the degummed silk fibers in a lithium bromide chaotropic solvent with a concentration higher than 9M, stir with a magnet at 60°C until comp...

Embodiment 3

[0065] A scaffold material for nerve regeneration based on silk fibroin fibers, characterized in that: the scaffold material is composed of silk fibroin, polyaniline and gelatin, the molecular weight of the silk fibroin is 200,000, and the weight content is 95%; The above-mentioned gelatin is white gelatin, the weight content is 4%, and the molecular weight is 70,000; the described polyaniline molecular weight is 20,000, and the weight content is 1%.

[0066] The scaffold material is prepared according to the following steps:

[0067] Step 1: Put silkworm cocoons into distilled water and cook for 30 minutes, take them out and soak them in 0.2% sodium carbonate and 0.025% polyethylene glycol octylphenyl ether solution for 4 hours to obtain degummed silk fibers, wash with distilled water, and dry overnight at 60°C ;

[0068] Step 2: Dissolve the degummed silk fibers in a lithium bromide chaotropic solvent with a concentration higher than 9M, stir with a magnet at 60°C until com...

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Abstract

The invention belongs to the technical field of biological materials, and particularly relates to a scaffold material for nerve regeneration based on silk fibroin fibers. Silk fibroin is obtain aftera series of treatments are performed on silkworm cocoons, and a silk fibroin solution is obtain after the silk fibroin is dissolved with formic acid; aniline reacts in a hydrochloric acid solution ofammonium persulfate to obtain polyaniline powder which is dissolved in part of the silk fibroin solution to obtain a polyaniline silk fibroin solution; and finally, the silk fibroin solution and the polyaniline silk fibroin solution are mixed for electrostatic spinning to obtain the scaffold material. The invention solves the problem that the silk fibroin has low electric conductivity and cannot be used for nerve repair, and the prepared scaffold material not only has high biocompatibility and mechanical property, but also has a high electrophilic property, and the conduction of a nerve conduit to an electric signal is not blocked.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a scaffold material for nerve regeneration based on silk fibroin fibers, more specifically, a fiber scaffold mainly composed of silk fibroin prepared by high-voltage electrospinning. Background technique [0002] Peripheral nerve damage from trauma or other neuropathy often results in impaired nerve perception or drive, with irreversible disabling consequences. Artificial nerve repair sleeves are usually made of biocompatible natural polymers and synthetic polymers into a sleeve shape to bridge nerves with gaps greater than 5mm, and by inducing the axon differentiation and sprouting of adjacent nerves and peripheral nerves to achieve nerve restoration. Repaired functionality. In order to keep the pulse signal accurate and effective after nerve repair, artificial casing materials often need to introduce conductive materials. Conductive materials such as polypyrr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/26
CPCA61L27/26A61L27/50A61L2430/32C08L89/00C08L79/02
Inventor 孟庆怡许杉杉赵亮亮黄健翔
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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