Artificial silk fibroin nano-fiber nerve repair conduit and preparation method thereof

A nerve repair, silk fibroin technology, applied in catheters, medical science, surgery, etc., can solve problems such as nerve cell migration not helping

Inactive Publication Date: 2009-11-18
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After Gu Xiaosong et al. dissolved the silk fibroin, the eggshell-structured catheter prepared by the mold was confirmed by in vivo experiments to promote the regeneration of nerve defects in mice (see: Xiaosong GU et al.Biomaterials.28(2007) 5526-5535) , but because the inner wall of the above-mentioned eggshell structure is a mesh structure, it is not helpful for the migration of nerve cells

Method used

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  • Artificial silk fibroin nano-fiber nerve repair conduit and preparation method thereof
  • Artificial silk fibroin nano-fiber nerve repair conduit and preparation method thereof
  • Artificial silk fibroin nano-fiber nerve repair conduit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) and stirring and dissolving in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis and drying, it becomes a regenerated silk fibroin film.

[0041] (2) The regenerated silk fibroin film prepared in step (1) was dissolved in formic acid, and the regenerated silk fibroin artificial nerve repair catheter was prepared by electrospinning.

[0042] (3) In step (2), through the rotation and movement of the roller device, a catheter with an inner layer parallel to the axial direction of the catheter and an outer layer surrounding the axial direction of the catheter is obtained. The specific method is: first, the rotation speed of the roller device is in a l...

Embodiment 2

[0045] (1) The natural tussah silk was boiled and degummed with 5% sodium carbonate solution for 30 minutes, and the pure tussah silk fibroin protein fiber was obtained after repeated 3 times.

[0046] (2) Dissolving the tussah silk fiber prepared in step (1) in lithium thiocyanate solution. Dialyzed with deionized water for 3 days, dried to become a regenerated tussah silk fibroin film.

[0047] (3) Dissolve the regenerated tussah fibroin protein film prepared in step (2) in HFIP, and obtain the regenerated tussah silk fibroin protein artificial nerve repair catheter by electrospinning method: first, the rotation speed of the drum device is 10r / min, the translation speed is 15cm / s, and parallel fibers are formed to form the inner conduit (300 microns in thickness); the translation speed is adjusted to 2 cm / s, and the rotation speed is 2200r / min, to form the outer fiber wall (300 microns in thickness).

[0048] (4) During the catheter spinning process in step (3), a catheter...

Embodiment 3

[0050] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin fibers were dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) and stirring and dissolving in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis and drying, it becomes a regenerated silk fibroin film.

[0051] (2) The regenerated silk fibroin film prepared in step (1) was dissolved in formic acid, and the regenerated silk fibroin artificial nerve repair catheter was prepared by electrospinning.

[0052] (3) In step (2), the structure in which the inner layer is parallel to the axial direction of the conduit and the outer layer of fibers is arranged perpendicular to the axial direction of the conduit is obtained through the rotation and movement of the collecting tube (same as the first embodiment).

...

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Abstract

The invention discloses a nerve repair conduit and a preparation method thereof. The nerve repair conduit consists of an inner layer conduit and an outer layer fiber wall, wherein the inner layer conduit consists of silk fibroin fibers arranged in the extending direction of the conduit, the outer layer fiber wall consists of the silk fibroin fibers encircling the conduit vertically and arranged axially, and the diameters of the silk fibroin fibers are between 50 and 3,000 nanometers; and the method for preparing the nerve repair conduit comprises the following steps: (1) preparing a regenerative silk fibroin film; (2) dissolving a pure regenerative silk fibroin film and preparing the silk fibroin fibers by using electrostatic spinning; and (3) collecting the silk fibroin fibers by using a roller device to obtain the nerve repair conduit. The nerve repair conduit is made from high-purity silk fibroin and is biodegradable, and the inner layer is made from parallel fibers arranged in the extending direction of the conduit and can conduct nerve cell migration; and the outer layer fibers are arranged by encircling the conduit axially and can support the conduit to prevent collapse.

Description

technical field [0001] The invention relates to a bridge for peripheral nerve and spinal cord injury and a preparation method thereof. Background technique [0002] Due to the accelerating pace of production and life in modern society, people are more likely to be harmed. Injuries due to accidents are often accompanied by damage to the peripheral nervous system. Nerve damage often results in long-term loss of function due to the inability of tissue to regenerate successfully. Currently, autologous nerve transplantation is usually used to treat severe peripheral nerve injury clinically. This method has a good repair effect and is the "gold standard" for repairing peripheral nerve defects. However, the autologous transplantation method also has many shortcomings, such as the limitation of the source of the donor nerve and the loss of function of the donor nerve. In order to overcome these problems, using artificial nerve conduits to induce regeneration of damaged nerves is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B17/11A61B17/00A61L29/04
Inventor 张锋左保齐
Owner SUZHOU UNIV
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