Separation and culturing method of ectomesenchyme stem cell

A technology of stromal stem cells and culture method, which is applied in the field of ectodermal mesenchymal stem cell separation and culture, and can solve the problems such as NCSCs that have not been separated and cultured yet.

Inactive Publication Date: 2005-03-09
STOMATOLOGICAL HOSPITAL NO 4 ARMY MEDICAL COLLEGE PLA
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  • Summary
  • Abstract
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Problems solved by technology

[0003] At present, the research on NCSC at home and abroad is mainly limited to the non-oviparous system, and there are no literature reports and related patents on the isolation and cultivation of NCSC.

Method used

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Examples

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Embodiment Construction

[0034] This embodiment is a method for isolating and culturing ectodermal mesenchymal cells of SD rat maxillary process, comprising the following steps:

[0035] 1. Isolation and primary culture of SD rat mandibular ectodermal mesenchymal cells

[0036] The SD rats at 11-12 days of pregnancy were killed by neck dislocation, and the fetal rats were taken out by laparotomy under aseptic conditions. A large amount of serum-containing culture solution was used to stop digestion, and the tissue was dispersed into a cell suspension by gently pipetting, centrifuged at 800 rpm for 6 minutes, the supernatant was discarded, and the culture solution was added to resuspend the cells. culture medium at 37°C, 5% CO 2 In the incubator for 40 minutes, observe under an inverted microscope that most of the cells have adhered to the wall, discard the supernatant, add fresh culture medium to continue the culture, observe the growth of the cells every other day, and replace the culture medium eve...

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Abstract

A process for separating and culturing the ectomesenchyme stem cells which can be used for bone tissue engineering, muscle tissue engineering, tooth tissue engineering and repairing peripheral nerve nicludes such steps as preparing culture medium, separating, culturing and purifying.

Description

technical field [0001] The invention belongs to the technical field of biological cells, and relates to a method for separating and culturing ectodermal mesenchymal stem cells. Background technique [0002] During the development of each protrusion of the maxillofacial region, neural crest cells (neural crest stem cells, hereinafter referred to as NCSC) derived from the junction of the neural tube and the surface ectoderm undergo directional migration and proliferation to reach the facial process mesoderm, called ectoderm. Embryo mesenchyme. During embryonic development, ectodermal mesenchymal stem cells can further differentiate into multiple lineages such as odontoblasts, dental pulp cells, osteoblasts, myoblasts, glial cells, and neurons. Compared with other stem cells used in tissue engineering (such as embryonic stem cells, bone marrow mesenchymal stem cells, neural crest cells and neural stem cells), it has the following advantages: ① rich source, easy to obtain mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/02A61L27/36A61L31/00C12N5/0735C12N5/0775
Inventor 金岩邓蔓菁张建平张光东
Owner STOMATOLOGICAL HOSPITAL NO 4 ARMY MEDICAL COLLEGE PLA
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