Serum-free cell culture fluid suitable for enriching and culturing tumour stem cells

A technology of serum-free culture medium and tumor stem cells, which is applied in the field of cell biology research, enrichment and cultivation of human tumor cells and tumor stem cells, can solve the problem of no tumor stem cells, etc., achieve good stem cell characteristics, clear experimental system, and applicable wide range of effects
CN101984051AActive Publication Date: 2011-03-09SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2011-03-09

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Abstract

The invention provides a serum-free cell culture fluid suitable for enriching and culturing tumour stem cells. DMEM / f12 serves as the basic culture fluid of the serum-free cell culture fluid and transferrin, insulin and other substances are also added as the components of the serum-free cell culture fluid. The serum-free cell culture fluid provided by the invention can efficiently enrich tumour stem cells from malignant tumour cell strains and tumour tissues. In addition, the serum-free cell culture fluid can promote stable growth of the tumour stem cells and maintain fine cell activity and physiological properties of the tumour stem cells, thus being very suitable for research fields related to tumour cells and tumour stem cells.
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Description

technical field

[0001] The invention relates to the field of cell biology research. More specifically, the present invention relates to the technical field of enrichment and cultivation of human tumor cells and tumor stem cells. Background technique

[0002] Tumor is a heterogeneous tissue. Tumor cells are affected by the complex microenvironment and continuously acquire gene mutations during the proliferation process, resulting in a variety of tumor cells with different phenotypes and different degrees of differentiation in tumor tissue. In recent years, more and more researchers support the hypothesis of cancer stem cells (Cancer Stem Cells, CSC) through the further study of tumors. CSC is a cell with self-renewal and self-replication ability existing in tumor tissue. CSC has strong tolerance to chemotherapy drugs and radiation therapy, and can differentiate into tumor cells of different phenotypes, rebuilding the heterogeneity of tumor tissue . In-depth research on CSC...

Claims

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