Serum-free cell culture fluid suitable for enriching and culturing tumour stem cells

A technology of serum-free culture medium and tumor stem cells, which is applied in the field of cell biology research, enrichment and cultivation of human tumor cells and tumor stem cells, can solve the problem of no tumor stem cells, etc., achieve good stem cell characteristics, clear experimental system, and applicable wide range of effects

Active Publication Date: 2011-03-09
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no culture medium for tumor stem cells on the market

Method used

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  • Serum-free cell culture fluid suitable for enriching and culturing tumour stem cells
  • Serum-free cell culture fluid suitable for enriching and culturing tumour stem cells
  • Serum-free cell culture fluid suitable for enriching and culturing tumour stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Alanine 4mg / L Arginine 134mg / L

[0076] Asparagine 6.8mg / L Aspartic Acid 6.5mg / L

[0077] Cysteine ​​16.1mg / L Cystine 30mg / L

[0078] Glutamic acid 7.0mg / L Glutamine 335mg / L

[0079] Glycine 16mg / L Histidine 31mg / L

[0080] Isoleucine 52.3mg / L Leucine 55.5mg / L

[0081] Lysine 90.8mg / L Methionine 16.5mg / L

[0082] Phenylalanine 33.25mg / L Proline 16.5mg / L

[0083] Serine 24.8mg / L Threonine 51.25mg / L

[0084] Tryptophan 8.5mg / L Tyrosine 54.35mg / L

[0085] Valine 50.55mg / L Biotin 0.003mg / L

[0086] Choline Chloride 7.8mg / L Folic Acid 2.2mg / L

[0087] Inositol 11.28mg / L Niacinamide 1.9mg / L

[0088] Calcium pantothenate 1.9mg / L Vitamin B6 1.9mg / L

[0089] Pyridoxine 0.03mg / L Riboflavin 0.2mg / L

[0090] Thiamine Hydrochloride 1.9mg / L Thymidine 0.315mg / L

[0091] Vitamin B12 0.6mg / L L-Ascorbic acid-2-phosphate 9.5mg / L

[0092] trisodium salt

[0093] Copper sulfate 0.001mg / L Ferric nitrate 0.045mg / L

[0094] Ferrous sulfate 0.38mg / L Magnesiu...

Embodiment 2

[0110] Alanine 5mg / L Arginine 155mg / L

[0111] Asparagine 8.2mg / L Aspartic Acid 7.5mg / L

[0112] Cysteine ​​18.3mg / L Cystine 35mg / L

[0113] Glutamic acid 8.0mg / L Glutamine 450mg / L

[0114] Glycine 19mg / L Histidine 33mg / L

[0115] Isoleucine 56.6mg / L Leucine 60mg / L

[0116] Lysine 96.5mg / L Methionine 18.5mg / L

[0117] Phenylalanine 37.75mg / L Proline 18.5mg / L

[0118] Serine 27.6mg / L Threonine 55.45mg / L

[0119] Tryptophan 9.6mg / L Tyrosine 57.25mg / L

[0120] Valine 55.75L Biotin 0.04mg / L

[0121] Choline Chloride 9.5mg / L Folic Acid 2.76mg / L

[0122] Inositol 13.46mg / L Niacinamide 2.1mg / L

[0123] Calcium pantothenate 2.3mg / L Vitamin B6 2.2mg / L

[0124] Pyridoxine 0.033mg / L Riboflavin 0.25mg / L

[0125] Thiamine Hydrochloride 2.3mg / L Thymidine 0.385mg / L

[0126] Vitamin B12 0.72mg / L L-Ascorbic acid-2-phosphate 12.5mg / L

[0127] trisodium salt

[0128] Copper sulfate 0.0015mg / L Ferric nitrate 0.065mg / L

[0129] Ferrous sulfate 0.42mg / L Magne...

Embodiment 3

[0146] Alanine 4.5mg / L Arginine 145mg / L

[0147] Asparagine 7.5mg / L Aspartic Acid 7.0mg / L

[0148] Cysteine ​​17.1mg / L Cystine 33mg / L

[0149] Glutamic acid 7.3mg / L Glutamine 350mg / L

[0150] Glycine 18mg / L Histidine 32mg / L

[0151] Isoleucine 55mg / L Leucine 57.1mg / L

[0152] Lysine 94.8mg / L Methionine 17.5mg / L

[0153] Phenylalanine 35.25mg / L Proline 17.5mg / L

[0154] Serine 25.0mg / L Threonine 52.5mg / L

[0155] Tryptophan 9.2mg / L Tyrosine 55.35mg / L

[0156] Valine 53.55mg / L Biotin 0.035mg / L

[0157] Choline Chloride 8.5mg / L Folic Acid 2.4mg / L

[0158] Inositol 12mg / L Niacinamide 2.0mg / L

[0159] Calcium pantothenate 2.0mg / L Vitamin B6 2.0mg / L

[0160] Pyridoxine 0.032mg / L Riboflavin 0.22mg / L

[0161] Thiamine Hydrochloride 2.1mg / L Thymidine 0.35mg / L

[0162] Vitamin B12 0.67mg / L L-Ascorbic acid-2-phosphate 10.2mg / L

[0163] trisodium salt

[0164] Copper sulfate 0.0012mg / L Ferric nitrate 0.055mg / L

[0165] Ferrous sulfate 0.40mg / L Ma...

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PUM

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Abstract

The invention provides a serum-free cell culture fluid suitable for enriching and culturing tumour stem cells. DMEM/f12 serves as the basic culture fluid of the serum-free cell culture fluid and transferrin, insulin and other substances are also added as the components of the serum-free cell culture fluid. The serum-free cell culture fluid provided by the invention can efficiently enrich tumour stem cells from malignant tumour cell strains and tumour tissues. In addition, the serum-free cell culture fluid can promote stable growth of the tumour stem cells and maintain fine cell activity and physiological properties of the tumour stem cells, thus being very suitable for research fields related to tumour cells and tumour stem cells.

Description

technical field [0001] The invention relates to the field of cell biology research. More specifically, the present invention relates to the technical field of enrichment and cultivation of human tumor cells and tumor stem cells. Background technique [0002] Tumor is a heterogeneous tissue. Tumor cells are affected by the complex microenvironment and continuously acquire gene mutations during the proliferation process, resulting in a variety of tumor cells with different phenotypes and different degrees of differentiation in tumor tissue. In recent years, more and more researchers support the hypothesis of cancer stem cells (Cancer Stem Cells, CSC) through the further study of tumors. CSC is a cell with self-renewal and self-replication ability existing in tumor tissue. CSC has strong tolerance to chemotherapy drugs and radiation therapy, and can differentiate into tumor cells of different phenotypes, rebuilding the heterogeneity of tumor tissue . In-depth research on CSC...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/095
Inventor 张雁张海霞
Owner SUN YAT SEN UNIV
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