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Small-caliber silk fibroin tubular material and preparation method thereof

A silk fibroin and small-caliber technology, applied in the direction of single-component protein rayon, types of packaging items, special packaging items, etc., can solve the problems of not fully meeting the sufficient conditions for vascular regeneration and repairing scaffolds, and achieve good biocompatibility , Conducive to adhesion, excellent physical and chemical properties

Inactive Publication Date: 2010-11-10
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method is beneficial to the adhesion of vascular endothelial cells, it does not fully meet the sufficient conditions as a scaffold for vascular regeneration and repair because it does not provide the corresponding support and environment for the repair of the middle and outer layers of blood vessels.

Method used

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  • Small-caliber silk fibroin tubular material and preparation method thereof
  • Small-caliber silk fibroin tubular material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] See attached figure 1 , which is a schematic diagram of the structure of a small-caliber silk fibroin tubular material forming die provided in this embodiment; the cylindrical intermediate body 1 is set on the outside of the hollow cylinder 2, and they are coaxially installed on the circular base 3; The diameter of the cylindrical intermediate body is 1.5 mm, the height is 5 cm, and it is connected with the circular base by screws; the hollow cylinder is 4 cm in height, and the distance between the cylindrical intermediate body and the cylindrical intermediate body is 0.3 mm, and it is engaged with the circular base through the card slot. Together.

[0026] This embodiment provides a small-diameter silk fibroin tubular material, and the preparation steps are as follows:

[0027] 1) Put 1 kg of cocoon shell into 50 liters of neutral soap solution with a mass concentration of 0.5%, and treat it at 98-100°C for 2 hours to degumming the cocoon shell, and obtain pure silk f...

Embodiment 2

[0038] 1) Put 1 kg of raw silk into 30 liters of 0.2% sodium carbonate aqueous solution, treat it at 98-100° C. for 2 hours, degumming the raw silk, and obtain pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin is dissolved with 8 liters of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 75±5° C. to form a silk fibroin mixed solution.

[0039] 2) Using a cellulose membrane as a dialysis material, dialyzing the obtained silk fibroin mixed solution with deionized water to remove impurities such as calcium chloride to obtain a pure silk fibroin solution, adjusting the mass concentration of the silk fibroin solution to 6%.

[0040] 3) Pour the silk fibroin solution into a polyethylene container, dry it at room temperature to obtain a silk fibroin film, and pulverize it to obtain a silk fibroin powder.

[0041] 4) Weigh 0.45 g of silk fibroin powder with an electronic balance, dissolve it in 2.55 g of anhydrous formic ac...

Embodiment 3

[0049] 1) Put 200 grams of cocoon shells into 10 liters of aqueous sodium carbonate solution with a mass concentration of 0.3%, and treat them at 98-100° C. for 2 hours to degumming the cocoon shells, and obtain pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin is dissolved with 100 ml of 9.3 mol / liter lithium bromide aqueous solution under stirring at 65±2° C. to form a silk fibroin mixed solution.

[0050] 2) Using a cellulose membrane as a dialysis material, dialyze the obtained silk fibroin mixed solution with deionized water to remove impurities such as lithium bromide to obtain a pure silk fibroin solution, and adjust the mass concentration of the silk fibroin solution to 9%.

[0051] 3) Pour the silk fibroin solution into a polyethylene container, dry it at room temperature to obtain a silk fibroin film, and pulverize it to obtain a silk fibroin powder.

[0052] 4) Weigh 0.9 g of silk fibroin powder with an electronic balance, dissolve...

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Abstract

The invention discloses a small-caliber silk fibroin tubular material and a preparation method thereof and belongs to the field of biomedical materials. The tubular material consists of three layers of materials which are firmly jointed, wherein an outer layer is a tubular electrostatic spinning silk net; a middle layer is a netlike pipe formed by weaving bright silk prepared from degummed silk; and an inner layer is a silk porous material. After the processes of soaking, drying, curing and the like in silk solution, the three layers of the material are firmly jointed, and the inner layer is treated by using anti-coagulation medicaments such as heparin and the like so that the material has coagulation resistance; and the tubular material obtained by using a forming die consisting of a cylindrical intermediate (1), a hollow cylinder (2) and a round base (3) has the inner diameter of between 1 and 8 millimeters, the thickness of between 0.2 and 2 millimeters, high mechanical property, biocompatibility, coagulation resistance and capability of repairing tissues such as blood vessels, nerves and the like.

Description

technical field [0001] The invention relates to a biomedical material that can be used for repairing tissues such as blood vessels and nerves, in particular to a small-caliber tubular material with good mechanical properties and biological properties and a preparation method thereof. Background technique [0002] Various pipeline systems of the human body often need to be repaired due to damage, disease and other reasons. Taking blood vessels as an example, tens of thousands of people around the world suffer from vascular diseases every year due to various reasons such as vascular aging, damage, and disease. Vascular defect repair, especially the repair of small-diameter vessels (diameter <6 mm) has always been one of the clinical difficulties. Small vessel defect repair includes traditional direct replacement and tissue regeneration methods (tissue engineering methods). Direct replacement of defective small blood vessels with biologically inert materials such as polyes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/56A61L27/22A61L33/10A61L33/04D01F4/00
Inventor 赵荟菁李明忠吴徵宇
Owner SUZHOU UNIV
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