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Liquid chromatogram and fluorescence method for simultaneously detecting aflatoxin B1, ochratoxin A, zearalenone and citrinin in grains

A technology for zearalenone and aflatoxin, which is applied in the field of liquid chromatography-fluorescence method, can solve the problems of long detection period, high cost, large pollution, etc., and achieves fast measurement speed, little environmental pollution, and low detection cost. Effect

Inactive Publication Date: 2012-08-15
INST OF AGRI QUALITY STANDARDS & TESTING TECH RES HUBEI ACADEMY OF AGRI SCI +2
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Problems solved by technology

However, due to the great differences in the physical and chemical properties of various mycotoxins, the existing analysis methods are all aimed at a single toxin or the same family of toxins with similar chemical structures, and have the disadvantages of long detection period, high cost, large pollution, and low efficiency. It is difficult to effectively prevent and control mycotoxins in grains; the existing multi-mycotoxin detection technology is usually based on high-performance liquid chromatography-tandem mass spectrometry and other cutting-edge equipment with high detection costs, but at this stage, such high-cost methods Detection technology is still difficult to promote at the grassroots level

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  • Liquid chromatogram and fluorescence method for simultaneously detecting aflatoxin B1, ochratoxin A, zearalenone and citrinin in grains
  • Liquid chromatogram and fluorescence method for simultaneously detecting aflatoxin B1, ochratoxin A, zearalenone and citrinin in grains
  • Liquid chromatogram and fluorescence method for simultaneously detecting aflatoxin B1, ochratoxin A, zearalenone and citrinin in grains

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0031] The present embodiment provides a method for simultaneously detecting aflatoxin B in grains 1 , the liquid chromatography-fluorescence method of ochratoxin A, zearalenone and citrinin, the method comprises the following steps:

[0032] 1 Extraction of toxins to be tested in grains

[0033] 1.1 Sample preparation

[0034] Grind the grain samples and pass them all through a 40-mesh sieve, mix well, and store them in the dark and at low temperature for later use.

[0035] 1.2 Preparation of general-purpose extract

[0036] Take 12 mL of glacial acetic acid, dilute to 100 mL with distilled water, shake well and set aside.

[0037] 1.3 Sample extraction

[0038] Accurately weigh 5 g of ground grain samples, accurate to 0.01 g, put them into a 50 mL capped centrifuge tube A, add 15 mL of general-purpose extraction solution, shake vigorously for 1 ...

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Abstract

The invention belongs to the technical field of food safety detection, and provides a high-efficient liquid chromatogram method for simultaneously detecting aflatoxin B1, ochratoxin A, zearalenone and citrinin in grains. Universal type extracting solution is extracted, detection is realized by a high-efficeint liquid chromatogram and fluorescence detector method, and quantification is realized by an external standard method. Detection limits of the method respectively include 1.08 micro-grams / kg of aflatoxin B1, 3 micro-grams / kg of ochratoxin A, 35.85 micro-grams / kg of zearalenone and 6.52 micro-grams / kg of citrinin. The method is simple, convenient and fast, is low in environmental pollution and detection cost, and is applicable to simultaneously and quantitatively detect four types of mycotoxin in the grains.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, in particular to a method for simultaneously detecting aflatoxin B in grains 1 , ochratoxin A, zearalenone and citrinin by liquid chromatography-fluorescence method. Background technique [0002] Mycotoxins refer to secondary metabolites secreted during the growth and metabolism of toxin-producing filamentous fungi that are harmful to human and animal health. Due to the wide distribution of such mycotoxins, some toxins also have teratogenic, carcinogenic and mutagenic effects, and have been listed by the World Health Organization as one of the three major causes of foodborne diseases in recent years. Because toxin-producing fungi can easily infect agricultural products before and after delivery under suitable conditions, resulting in mycotoxin pollution, which threatens the health of humans and livestock, and also brings huge economic losses. At present, although countries around ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 周有祥陈福生王小红董丽娜陈建彪路磊李书谦胡定金
Owner INST OF AGRI QUALITY STANDARDS & TESTING TECH RES HUBEI ACADEMY OF AGRI SCI
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