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Fabrication method and application for citrinin immune chromatography detection test paper

A technology for immunochromatography detection and production method, which is applied in the field of production of citrinin immunochromatography test paper, can solve the problems of being unable to be popularized and used, harmful radioactive substances, etc., and achieves the effects of simple operation, high sensitivity, and low cost

Inactive Publication Date: 2005-04-06
AIBIT BIOTECH INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the RIA method has high sensitivity, it cannot be widely used due to the use of radioactive substances harmful to the human body.

Method used

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  • Fabrication method and application for citrinin immune chromatography detection test paper
  • Fabrication method and application for citrinin immune chromatography detection test paper
  • Fabrication method and application for citrinin immune chromatography detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A citrinin immunochromatographic detection test paper, comprising a liner 1, a sample pad 2, a tracer particle conjugate pad 3, a nitrocellulose membrane 4, an absorbent pad 5, a detection line 6, a quality control line 7, and the liner On 1, there are sample pad 2, tracer particle conjugate pad 3, nitrocellulose membrane 4, and water-absorbing pad 5 in sequence; tracer particle conjugate pad 3 has anti-ciritrinin antibody 10 labeled with tracer particle 9, The nitrocellulose membrane 4 is also provided with a detection line 6 and a quality control line 7, the detection line 6 has a citrinin detection antigen 8, and the quality control line 7 has an anti-antibody 11, wherein the anti-antibody 11 is an anti-citrinin Antibody to Antibody 10.

[0032] The detection principle is as follows: add sample 12 dropwise on the sample pad 2, if there is citrinin in the sample 12, the citrinin will move to the tracer particle conjugate pad 3 through chromatography, and the limited a...

Embodiment 2

[0035] The tracer particles 9 are colloidal gold particles. All the other are with embodiment 1.

Embodiment 3

[0037] The tracer particles 9 are latex particles. All the other are with embodiment 1.

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Abstract

This invention discloses a citrinin immune chromatography test paper and its process method and utility. The test paper comprises pad, sample pad, tracing particle compound pad, pyroxylin film, adsorption pad, test thread, and control thread. The method is the following: to orderly put sample pad, tracing particle compound pad, pyroxylin film and adsorption pad on the pad; the tracing particle compound pad has citrinin antibody with tracing particle mark; the pyroxylin film has test thread and control thread; the test thread has citrinin antigen and antibody of citrinin antigen.

Description

technical field [0001] The invention belongs to the field of biotechnology, and discloses a production method and application of a citrinin immunochromatographic detection test paper. Background technique [0002] Citrinin is a mycotoxin produced by certain strains of Penicillium and Aspergillus, which was first isolated and purified in 1931. It can cause significant nephrotoxicity and is carcinogenic. The survey found that some citrinin-producing bacteria are widely distributed in nature and often cause mildew in crops such as corn and rice. In recent years, the pollution of citrinin has attracted more and more attention. In the Symposium on Mycotoxins, Endemic Nephropathy and Urinary Tract Tumors held in Lyon, France in 1991, the role of citrinin in the occurrence of Balcan endemic nephropathy was discussed, which aroused the great attention of the International Cancer Research Society. At that time, citrinin was listed as one of the toxins that must be detected by the ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/532G01N33/543G01N33/558
Inventor 许杨刘仁荣熊勇华赖卫华陈高明
Owner AIBIT BIOTECH INSTR
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