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In-situ rapid detection method for acetamiprid in vegetables or fruits

A detection method, acetamiprid technology, applied in the field of food science, can solve problems such as complex methods, and achieve the effect of simple method, fast processing speed and low cost

Pending Publication Date: 2022-01-14
上海市农产品质量安全中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a rapid in-situ detection method for acetamiprid in vegetables or fruits. The above-mentioned rapid in-situ detection method for acetamiprid in vegetables or fruits should solve the current There are complex technical problems in the detection method of acetamiprid in vegetables or fruits in the art

Method used

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  • In-situ rapid detection method for acetamiprid in vegetables or fruits
  • In-situ rapid detection method for acetamiprid in vegetables or fruits

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Sample preparation

[0023] The sampling amount of vegetables and fruits shall be carried out in accordance with GB / T8855. Random sampling of 1 kg of edible fungus samples shall be carried out in accordance with the provisions of GB 2763. After taking samples, chop them up, mix them thoroughly, and use the quartering method to sample or put them directly. Put it into a tissue masher and mash it into a homogenous slurry, and put it into a polyethylene bottle.

[0024] (2) QuEChERS extraction

[0025] Weigh 10g of sample into a 50mL plastic centrifuge tube, add 20mL of acetonitrile, shake for 2min, then add extraction salt bag and 1 ceramic homogenate to the filtrate, shake vigorously for 1min, and let stand at room temperature for 1-3min.

[0026] Further, the extraction salt package is composed of 4g of anhydrous magnesium sulfate, 1g of sodium chloride, 1g of sodium citrate, and 0.5g of disodium hydrogen citrate.

[0027] (3) m-PFC purification

[0028] Take 1.5...

Embodiment 2

[0035] The instrument used in the following examples is a high-efficiency thin-layer pesticide residue tester provided by Shanghai Ruixin Technology Instrument Co., Ltd. The principle of the rapid measuring instrument is to place the sample under a purple low-pressure mercury lamp (254nm), adopt high-sensitivity spectral image recognition technology, and automatically perform two-way scanning imaging to quantitatively analyze acetamiprid.

[0036] Six kinds of fresh vegetables, including Chinese cabbage, tomato, cucumber, green cabbage, water spinach, and head cabbage, were selected as substrates. After the fresh samples were minced, they were directly put into a tissue masher to be mashed into a homogenate, and then put into polyethylene bottles. Weigh 6 samples of each matrix, weigh 10g of each sample into a 50mL plastic centrifuge tube, add 20mL of acetonitrile, add extraction salt bag and 1 ceramic homogenizer, shake vigorously for 1min, and let stand at room temperature f...

Embodiment 3

[0042] The instruments used in the following examples are the high-efficiency thin-layer pesticide residue analyzer provided by Shanghai Ruixin Technology Instrument Co., Ltd. and the Xevo TQ-S liquid mass tandem mass spectrometer provided by Waters Technology (Shanghai) Co., Ltd.

[0043] Cucumber and green vegetables were selected as substrates, and after being treated respectively according to the method of the present invention and national standards GB 3200.121-2021 and GB / T 20769-2008, the residual amount of acetamiprid was determined.

[0044]As can be seen from Table 2, there was no significant difference in the recovery rate and standard deviation of acetamiprid among the three methods. But standard methods require large instruments and are not suitable for rapid testing. In this experiment, the m-PFC column was used, the extraction and purification were completed in one step, the amount of reagents was small, the extract did not need to be dried and concentrated, the...

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Abstract

The invention provides an in-situ rapid detection method for acetamiprid in vegetables or fruits, which comprises the following steps of: preparing a sample; namely, putting a sample into a tissue pounding machine to be pounded into homogenate, and putting the homogenate into a polyethylene bottle; performing an extraction step: weighing 10g of the sample, putting the sample into a 50 mL plastic centrifuge tube, adding 20mL of acetonitrile, adding an extraction salt bag and homogeneous protons, vigorously shaking the mixture, and performing standing at room temperature; performing a purification step: sucking the supernatant, passing through a filtration type purification column, passing through a microfiltration membrane after primary purification, and collecting the filtrate; performing an enrichment step: after vortex centrifugation, sucking a bottom organic phase by using a microsyringe, and placing the bottom organic phase in a centrifuge tube; and performing a step of thin-layer chromatography detection; namely, absorbing the filtrate by using a capillary tube, dotting a plate, performing developing by using a developing solvent, and performing imaging analysis. According to the invention, on-site in-situ rapid detection of acetamiprid in vegetables or fruits can be realized.

Description

technical field [0001] The invention belongs to the field of food science and relates to a method for detecting pesticides, in particular to an in-situ rapid detection method for acetamiprid in vegetables or fruits. Background technique [0002] Acetamiprid is the third excellent chlorinated neonicotinoid insecticide discovered after imidacloprid and nitenpyram. In recent years, acetamiprid has been widely used in vegetable crops such as cucumbers. The maximum residue limit value of acetamiprid in fruit and vegetable vegetables currently stipulated by the European Union is 0.3mg / kg, and the MRL value of acetamiprid on cucumbers stipulated by Japan is 5mg / kg. kg, my country's latest regulation in GB2763-2019 is that the MRL value of acetamiprid in cucumber is 1mg / kg. At present, the commonly used detection methods for the analysis of acetamiprid residues include gas chromatography, high performance liquid chromatography, gas chromatography-mass spectrometry and liquid chromat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/28G01N1/34G01N1/40
CPCG01N30/90G01N1/286G01N1/34G01N1/4055G01N2001/2873G01N2001/4061
Inventor 丰东升张维谊梅博王敏王霞汪弘康陈美莲沈斯文高猛峰
Owner 上海市农产品质量安全中心
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