Method for preparing low citrinin monascus pigment from rice

A technology of monascus pigment and citrinin, applied in the field of preparation of low-citrinin monascus pigment, can solve technical barriers and other problems

Inactive Publication Date: 2008-12-17
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regardless of the solid-state fermentation extraction method or the liquid-state fermentation extraction method, the red yeast rice pigment produced contains more citrinin which is harmful to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Weigh 3 kilograms of rice, break it into 80 mesh fineness, add 12 times of drinking tap water, soak it in a fermenter for 10 minutes, add amylase equivalent to 0.2% of the weight of rice, and keep it warm for 70 minutes at 45°C to obtain Rice milk; (2) add ammonium nitrate (NH 4 NO 3 ), 0.25% potassium dihydrogen phosphate (KH 2 PO 4 ), 0.1% manganese sulfate (MnSO 4 ), 2% corn steep liquor, 1.5% soy flour, adjust the pH to 6.0 with lactic acid, heat to 121°C for 20 minutes, and cool to 35°C to obtain a fermentation broth; Aspergillus species, after being cultivated on a solid slant, inoculated into the seed medium and cultivated for 50 hours at 33° C., to obtain the enlarged culture solution of Monascus species; Add the fermented culture solution cooled to 35°C to the expansion culture solution of Monascus strains, incubate and stir culture at 31°C for 20 hours, then add 0.2% caprylic acid and 0.1% methyl ketone equivalent to the weight of rice, and continue to...

Embodiment 2

[0019] (1) Weigh 5 kg of rice, break it into 80 mesh fineness, add 12 times of drinking tap water, soak it in a fermenter for 8 minutes, add 0.15% amylase equivalent to the weight of rice, and keep it warm at 45°C for 80 minutes to obtain rice (2) add ammonium nitrate, 0.15% potassium dihydrogen phosphate, 0.15% manganese sulfate, 1.5% corn steep liquor, 2% soybean powder that are equivalent to 0.2% of rice milk weight in rice milk, adjust pH with lactic acid to be 6.0, Heating to 121°C for 20 minutes, cooling to 40°C to obtain a fermentation broth; (3) Expanded cultivation of Monascus strains: after cultured on a solid slant, the Monascus strains were inoculated into the seed medium at 32°C Cultivate down for 50 hours, make the production and expand the nutrient solution of Monascus strains; (4) Fermentation culture: add the fermentation nutrient solution that is cooled to 40 ℃ with the expansion nutrient solution of Monascus bacterium that is equivalent to 10% of fermentation...

Embodiment 3

[0022] (1) Weigh 4 kg of rice, break it into 80 mesh fineness, add 12 times of drinking tap water, soak it in a fermenter for 10 minutes, add 0.12% amylase equivalent to the weight of rice, and keep it warm at 40°C for 60 minutes to obtain rice (2) add ammonium nitrate, 0.25% potassium dihydrogen phosphate, 0.2% manganese sulfate, 1.0% corn steep liquor, 2.5% soya bean powder that are equivalent to rice milk weight 0.15% in rice milk, adjust pH with lactic acid to be 6.5, Heating to 121°C for 20 minutes, cooling to 37°C to obtain a fermentation broth; (3) Expanded cultivation of Monascus species: the Monascus strains were cultured on a solid slant and inoculated into the seed medium at 33°C Cultivate down for 50 hours, make the production and expand the nutrient solution of Monascus strains; (4) Fermentation culture: add the fermentation nutrient solution that is cooled to 37 ℃ with the expansion nutrient solution of Monascus bacterium that is equivalent to 9% of fermentation n...

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Abstract

The invention relates to a method for producing monascus pigment with low content of citrinin by using rice as raw material, and the process thereof consists of the following steps: (1) rice milk is prepared; (2) the rice milk is added with 0.1 percent to 0.2 percent of ammonium nitrate, 0.15 percent to 0.25 percent of potassium dihydrogenphosphate, 0.1 percent to 0.2 percent of manganese sulfate, 1 percent to 2 percent of maize pulp and 1.5 percent to 2.5 percent of soybean powder, and the obtained mixture is heated to 121 DEG C to be kept for 20 minutes, and then cooled to 35 DEG C to 40 DEG C to obtain a fermentation culture solution; (3) monascus strains are transferred to a seed culture medium to be cultivated for 50 hours under the temperature of 31 DEG C to 33 DEG C, after being cultivated on a solid slant plane, and then an enlarged monascus culture solution is obtained; (4) 8 percent to 10 percent of the enlarged monascus culture solution is fed into the fermentation culture solution, and the obtained mixture is kept under the temperature of 31 DEG C to 37 DEG C, stirred and cultivated for 20 hours, hereafter, octanoic acid with the weight equal to 0.1 percent to 0.2 percent of the weight of rice and methyl ketone with the weight equal to 0.1 percent to 0.2 percent of the weight of rice are added, then the temperature is kept, and the mixture is stirred and cultivated for 85 hours to 95 hours, while the pressure of a fermentor is kept to be 0.04MPa to 0.06MPa so as to obtain a fermentation liquor; (5) the fermentation liquor is filtered, and the obtained filtrate is treated with spray drying or freeze drying after membrane concentration. The monascus pigment obtained by the method consists of less than 0.05 mg/kg of the monascus when color value is higher than 200.

Description

technical field [0001] The invention relates to a method for preparing a low-citrinin monascus pigment, in particular to a method for preparing a low-citrinin monascus pigment by using rice as a raw material through microbial liquid fermentation technology. Background technique [0002] Red yeast rice is a traditional Chinese medicine and food fermented rice product. It has multiple functions such as promoting blood circulation and removing stasis, invigorating spleen and eliminating food, and promoting metabolism. It has a history of more than 1,000 years in my country. Rice is used as raw material to be dried and pulverized after solid-state fermentation. [0003] Monascus pigment is the main component of red yeast rice, and its use is very wide: ① as a food additive (natural colorant); ② as a raw material for health care medicine; ③ as a green chemical raw material. [0004] Monascus pigment is a mixture of various substances. So far, the identified monascus pigments incl...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/66
Inventor 林亲录
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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