Penicillium citrinum sourced citrinin compound (penicitrinol O) as well as preparation method and application thereof

A compound, the technology of Penicillium citrinum, is applied in the field of citrinin compounds to achieve the effect of significant anti-tumor activity

Inactive Publication Date: 2015-04-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the citrinin compounds shown have anti-tumor activity, and there is no report on the inhibitory activity of the compound on cell proliferation, so there is no drug related to it in the market.

Method used

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  • Penicillium citrinum sourced citrinin compound (penicitrinol O) as well as preparation method and application thereof
  • Penicillium citrinum sourced citrinin compound (penicitrinol O) as well as preparation method and application thereof
  • Penicillium citrinum sourced citrinin compound (penicitrinol O) as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Fermentative production and separation and purification of the compound of embodiment 1

[0019] 1 Fermentation production

[0020] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013713) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.

[0021] Get the Penicillium citrinum ( Penicillium citrinum ) appropriate amount of IBPT-5, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 30.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth were obtain...

Embodiment 2

[0029] Example 2 Test of anti-tumor activity in vitro

[0030] 1 Experimental samples and experimental methods

[0031] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0032] Cell lines and cell subculture Tumor cell lines were used, and tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0033] Cell Proliferation Inhibitory Activity Test Method

[0034] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. Add 2 microliters of sample solution...

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Abstract

The invention relates to a penicillium citrinum sourced citrinin compound (penicitrinol O) as well as a preparation method and an application thereof. The compound has an effect on inhibiting tumor cell proliferation and has anti-tumor activity, and the structural formula is shown in the specification. The preparation method comprises the steps: fermenting and culturing penicillium citrinum IBPT-5 to obtain a fermented product; and then separating and purifying the fermented product to obtain the compound. The experiment shows that the compound has high anti-tumor activity for human cervical cancer cell line hela and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for the study on anti-tumor.

Description

technical field [0001] The invention relates to a citrinin compound penicitrinol O derived from Penicillium citrinum and its preparation method and application. Background technique [0002] Fungi are relatively higher among microorganisms. Fungal natural products are an important source of activity diversity and structural diversity, and many chemical drugs with good therapeutic effects have been developed. For example, antibiotic penicillin (penicillin) series, immunosuppressant cyclosporin A (cyclosporin A), blood lipid-lowering drugs mevastatin (mevastatin), lovastatin (lovastatin) and antifungal drug griseofulvin (griseofulvin), etc. , These all show that fungus is an important treasure house of natural medicine. Citrinin, a well-known secondary metabolite of polyketide fungi, was first isolated from a secondary metabolite of Penicillium citrinum. Studies in recent years have found that citrinin compounds exhibit promising biological activities such as anti-tumor, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/06A61K31/352A61P35/00C12P17/18C12R1/80
CPCC07D493/06C12P17/181
Inventor 陈立毕延雪周彤张其清
Owner FUZHOU UNIV
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