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Human polycystic albumen -1 quantitative determination kit

A polycystin and quantitative detection technology, applied in the field of medical immunology detection, can solve the problems of high technical requirements for auxiliary inspection equipment and complicated operation.

Inactive Publication Date: 2004-11-10
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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AI Technical Summary

Problems solved by technology

The results of genetic diagnosis are accurate and reliable, but the technical conditions and auxiliary examination equipment are high, and the operation is cumbersome. At present, there is no body fluid diagnostic index for ADPKD clinically. There is an urgent need for a simple, convenient, easy-to-promote, sensitive and specific clinical diagnosis of ADPKD. Detection method

Method used

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Experimental program
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Embodiment Construction

[0019] Preparation and source of each reagent in the human PC-1 quantitative detection kit

[0020] 1. Preparation of standard protein, fusion protein PC-1-e2

[0021] The standard protein is human PC-1 N-terminal fusion protein PC-1-e2 freeze-dried powder, which corresponds to the 46th to 202th amino acid of the N-terminal flank-LRR-flank region of the extracellular region of human PC-1 and part of the WSC region Residue, the purity is above 97%. For the preparation process of the standard protein, refer to the invention patent "Human polycystin-1 N-terminal fusion protein PC-1-e2" filed on September 25, 2003, with the patent application number 03151184.8. Now its preparation process is briefly described as follows:

[0022] (1) Preparation of total RNA from human kidney tissue

[0023] Fresh healthy adult kidney tissue was taken, and total RNA was extracted by the conventional guanidine isothiocyanate method with a total RNA extraction kit from Shanghai Huashun Company. ...

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Abstract

The invention relates to a reagent box to quantitatively detect multivesicular protein-1 content in human body fluid, using anti-human multivesicular protein-1 N-end monoclonal antibody and anti-human mulitvesicular protein-1 N-end polyclonal antibody, adopting immunologic technique to establish a double-antibody filled enzyme linked immunosorbent assay (ELISA) method of quantitatively detecting body fluid and multivesicular protein-1 content in tissue cracking liquid. Comparing the difference in multivesicular protein-1 content in body fluid, judge if the patient has autosomal dominant hereditary nephropathy and has an important reference value in clinic diagnosis of this disease and provides an effective route to prevent and cure this disease as soon as possible.

Description

technical field [0001] The invention relates to the technical field of medical immunology detection, and is a double-antibody sandwich ELISA kit for quantitative detection of polycystin-1 content in human body fluid. Background technique [0002] Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common fatal monogenic genetic diseases in humans, involving all races, with a worldwide incidence of 1 / 500-1 / 1000 , affecting approximately 13 million people worldwide. It is characterized by the progressive occurrence and enlargement of bilateral renal cysts, and the disease gradually progresses, often developing into end-stage renal failure in the late middle age of the patient. Renal failure caused by ADPKD accounts for more than 10% of the total number of renal replacement therapy patients. This cystic disease is very harmful, not only occurs in the kidney, but also often involves extrarenal organs, causing polycystic liver...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/535G01N33/577
Inventor 梅长林赵海丹
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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