The invention discloses a passive type body liquid sample screening and examining device based on paper diaper and a using method thereof, relates to an examining device of body liquid sample, and aims to solve the problem that the conventional device is inconvenient to use, a sample with a small volume cannot be measured accurately and reliably, and the results cannot be maintained for a long time. From the top to bottom, the device sequentially comprises a surface layer made of a hydrophilic material, a collecting layer made of a hydrophobic material, a test layer made of a hydrophilic material, a protective layer made of a hydrophobic material, and a read layer made of a hydrophobic material; the collecting layer comprises a central outlet arranged on the center of the collecting layer, and a self locking switch arranged on the center outlet; and the test layer comprises a test layer body, a route network for passing fluids, and a detection reaction reagent block arranged in the route network. The provided device can detect small volume samples, is capable of detecting multiple markers, and has the advantages of reliable test results, long retaining time of results, and user-friendliness.