High-throughput algae culture and test method

A testing method and technology of an algae cultivation system are applied in the field of high-throughput algae cultivation and testing, which can solve the problems of difficulty in meeting the scientific research needs of algal cells, high dependence on manpower, time-consuming and laborious, and save research time and manpower costs. The system is small and the effect of improving the accuracy

Pending Publication Date: 2019-06-11
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to overcome the problems of high dependence on manpower in the traditional algae growth test in the prior art, time-consuming and laborious, poor stab

Method used

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  • High-throughput algae culture and test method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] This example is to illustrate the high-throughput algae cultivation and testing method of the present invention.

[0077] combine figure 1 with figure 2 ,specifically:

[0078] (H1) Cultivate algae in the photobioreactor array 10 in the high-throughput algae cultivation system;

[0079] (H2) The first stepper motor 3 drives the probe 4 to move horizontally, front-to-back, and vertically on the screw guide rail 2 at 5mm / min, and the second stepper motor 8 passes through the tooth groove structure on the gear and positioning belt III Mesh together, drive the displacement platform 1 to move horizontally, front-to-back and vertically at 5mm / min on the positioning belt III, and then drive the moving distance of the probe 4 to realize the precise positioning of the probe 4;

[0080] (H3) By controlling the one-way valve 7 on the sampling pump 5, sampling is realized through the flexible pipe 6. After sampling, the sampling pump 5 is idling, and all the liquid taken can be...

Embodiment 2

[0087] Carry out high-throughput algae culture and test according to the same method as Example 1, the difference is: the first stepping motor 3 drives the probe 4 on the screw guide rail 2 at 4mm / min in the horizontal, front and back and vertical directions Move, the second stepping motor 8 meshes with the cogged structure on the positioning belt III through the gear, and drives the displacement platform 1 to move horizontally, front-to-back and vertically at 4 mm / min on the positioning belt III; the photobioreactor array 10 The number of layers is 5 layers, and each layer includes 24 reactor units.

[0088] Results The automatic sampling frequency of the sampler can reach 2 samples / min, the sampling volume is 100 mL, and the sampling precision is 105 μL.

Embodiment 3

[0090] Carry out high-throughput algae culture and test according to the same method as Example 1, the difference is: the first stepper motor 3 drives the probe 4 on the screw guide rail 2 at 3mm / min in the horizontal, front and rear and vertical directions To move, the second stepper motor 8 meshes with the cog structure on the positioning belt III through the gear, and drives the displacement platform 1 to move horizontally, front and rear, and vertically at 3mm / min on the positioning belt III.

[0091] Results The automatic sampling frequency of the sampler can reach 2 samples / min, the sampling volume is 90 mL, and the sampling accuracy is 100 μL.

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Abstract

The invention relates to the technical field of culture and analysis of microorganisms, and discloses a high-throughput algae culture and test method. The method comprises the following steps: (S1) culturing algae in a high-throughput algae culture device; (2) caring out automatic sampling by an automatic sampling device from the high-throughput algae culture system and conveying the obtained sample to an analysis and detection device for detection; and (3) according to a test result of the analysis and detection device in the step (2), regulating and controlling culture conditions through a culture parameter feedback regulation device. According to the method, high-throughput and intelligent algae culture, rapid and automatic sampling detection, automatic sampling analysis in the cultureprocess and feedback control over culture conditions can be achieved.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation and analysis, in particular to a high-throughput algae cultivation and testing method. Background technique [0002] The cultivation and testing of algae is an essential part of the research and application of phycology. In the research of algae bioenergy, ecotoxicology, water eutrophication, water body plankton community structure and many other fields, generally by setting a normal control group Compared with the treatment group, for example, by adding toxic and harmful components such as heavy metals; adding sewage with high N and P content; adding different doses of nutrient salts; adding different algal species; adding different plankton, etc., comparing the growth of the two groups of algal cells Determination of important evaluation indicators such as algal biomass, oil pigment content, photosynthetic efficiency, and culture medium composition provides data support for the...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/36C12M1/34C12M1/26C12M1/04
Inventor 张学治沙君刘青玲肖敬荣
Owner INST OF AQUATIC LIFE ACAD SINICA
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