The invention discloses a
reference gene related to regulation and control of stress-resistant
gene expression of
arabidopsis thaliana and application of the
reference gene, and relates to the technical field of
genetic engineering, the
reference gene is LHCB5 and / or LHCB4.1, the
nucleotide sequence of the LHCB5 is shown as SEQ ID NO.1, and the
nucleotide sequence of the LHCB4.1 is shown as SEQ ID NO.2. The invention further discloses a preparation method of the reference
gene related to the regulation and control of stress-resistant
gene expression of
arabidopsis thaliana and application of the reference gene related to the regulation and control of stress-resistant
gene expression of
arabidopsis thaliana. Various analysis methods are used for analyzing and finding that
reference genes LHCB5 and LHCB4.1 show consistent stability under the high-
temperature stress condition of
arabidopsis thaliana, and when the LHCB5 and the LHCB4.1 are independently used as
reference genes and the combination of the LHCB5 and the LHCB4.1 is used as the
reference genes for relative quantification, the relative expression quantity of the HSFA2 gene under the high-
temperature stress condition is increased and has a consistent trend; it is verified that both the LHCB5 and the LHCB4.1 and the combination of the LHCB5 and the LHCB4.1 can be used as reference genes for qPCR analysis of the
arabidopsis thaliana under high
temperature stress, a reliable reference standard is provided for
gene expression research of the
arabidopsis thaliana under high temperature stress in the future, and the accuracy and comparability of experimental data can be improved.