Type 1 diabetes related antigen-antibody simultaneous detection egg white slice

A type 1 diabetes, antigen-antibody technology, applied in the field of life science immunohistochemical protein chip, can solve the problems of inconvenient diabetes typing and many antibodies, and achieve the effect of shortening the time of diagnosis, saving the amount, and saving manpower and material resources.

Inactive Publication Date: 2003-10-15
GENETECH BIOTECH SHANGHAI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] Since the diagnosis of type 1 diabetes involves many antibodie

Method used

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  • Type 1 diabetes related antigen-antibody simultaneous detection egg white slice
  • Type 1 diabetes related antigen-antibody simultaneous detection egg white slice
  • Type 1 diabetes related antigen-antibody simultaneous detection egg white slice

Examples

Experimental program
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Embodiment Construction

[0017] Such as figure 1 The present invention detects antibody schematic diagram and figure 2As shown in the antigen detection principle diagram of the present invention, the protein chip for simultaneous detection of type 1 diabetes-related antigens and antibodies is to place a variety of different antigens and antibodies on different positions on the solid-phase carrier, and the antigens and antibodies are connected to the solid-phase carrier through chemical bonds. After the surface of the solid phase carrier is blocked, add the sample to be tested, perform hybridization reaction with the immobilized biomolecules on the solid phase carrier, wash off the unbound substances, add the labeled secondary antibody and the labeled specific antibody mixture, and then carry out Hybridization, detection of marker signals after washing away unbound substances, characterized in that: the different antigens are mumps virus antigen (Mumps virus), Coxsackie virus B4 antigen (Coxsakievirus...

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Abstract

The present invention is protein chip for simultaneous detection of type-I diabetes relevant antigen and antibody, and relates to immunohistochemical protein chip in life science. The protein chip has on solid carrier different antigen and antibody lattices and antigen and antibody chemical bonds connected to the solid carrier. After the surface of the solid carrier is closed, tested specimen is added to produce hybridizing reaction with biological molecules fixed on the solid carrier. After the uncombined matter is washed off, the mixture of labeled double antibody and labeled specific antibody for further hybridization and the label signal is detected after washing off uncombined matter again. The said different antigens include mumps virus antigen, glomerular basement membrane antibody, etc. The present invention may be used in accurate and fast diagnosis of type-I diabetes.

Description

technical field [0001] The invention discloses a protein chip for simultaneous detection of type 1 diabetes related antigen and antibody, relates to a protein chip, application and preparation method, and belongs to the field of life science immunohistochemical protein chip. Background technique [0002] Diabetes (Diabetes Mellitus) is a complex disease of multiple etiologies. It is a combination of glucose, protein and lipid metabolism disorders caused by insulin deficiency or insulin-antagonizing hormone increase, or the inability of insulin to exert normal physiological functions in target cells. sign. Its basic feature is chronic hyperglycemia. In July 1997, the World Health Organization revised the diagnostic criteria and types of diabetes according to the revised opinion proposed by the American Diabetes Association. Mediated and idiopathic; 2. Type 2 diabetes: mainly insulin resistance with relative insulin deficiency, or insulin secretion defect with insulin resist...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/543G01N33/66
Inventor 张涛李宾彭永济邓富桥葛海鹏孙露
Owner GENETECH BIOTECH SHANGHAI
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