Tissue engineered fine particle tissue and method for preparing the same

A technology of microparticle tissue and tissue engineering, applied in medical science, prosthesis, etc., can solve the problem that the cells in the central part cannot get enough nutrients supply, there is no public information on tissue engineered microparticle tissue, and the metabolites are difficult to remove in time, etc. problem, to achieve good clinical treatment effect, good cell growth state, easy to use effect

Inactive Publication Date: 2008-06-11
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

The traditional method of tissue engineering technology to construct artificial tissues/organs is to combine seed cells with scaffold materials. When the volume of cultured tissues/organs is large, the cells in the center cannot get enough nutrients, and t

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  • Tissue engineered fine particle tissue and method for preparing the same

Examples

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example 1

[0016] Example 1: Preparation of tissue engineered microparticle skin tissue

[0017] 1. Preparation of acellular dermal matrix (extracellular matrix) microcarriers: Take fresh back skin of pigs, cut into 5cm×10cm in size, take skin in reverse direction with a skin-taking drum, cut off the epidermis about 0.3mm thick skin slice, expose The dermis part; use the leather drum to cut out the dermis with a thickness of about 0.3mm, and set it aside.

[0018] Put the pig dermis in 2.5g / L trypsin solution to digest, after cleaning, put the pig dermis in -80℃ freezer for more than half an hour to ensure that the temperature inside and outside the dermis reaches the same; after taking it out, thaw naturally at room temperature, and freeze and thaw repeatedly for 2~ 5 times to make the cells completely rupture and disintegrate; after washing with water, soak the corium in 4% NaOH solution, replace the NaOH solution every 6 hours, and soak for 24 hours; then use PBS solution (phosphate b...

example 2

[0021] Example 2: Preparation of tissue engineered microparticle adipose tissue

[0022] 1. Preparation of gelatin microcarriers: refer to the literature [Wang Yijuan et al. Preparation of gelatin-based slow-release microspheres as cell microcarriers. Chemical Journal of Chinese Universities, 2007 28(9): 1776-1780] to prepare gelatin microcarriers, and to screen particles Microcarriers with a diameter of 250 μm to 350 μm were sterilized with cobalt 60 and stored for later use.

[0023] 2. Preparation of tissue-engineered adipose tissue microparticles: Under sterile conditions, soak 2 grams of gelatin microcarriers in 20 ml of cell culture medium (DMEM culture medium containing 10% fetal bovine serum) for 24 hours to make them fully swell, and remove them by centrifugation supernatant; then add 5ml of 1mg / ml polylysine solution to soak the microcarriers for 2 hours, discard the liquid; dry naturally under sterile conditions.

[0024] will contain 1×10 5 10ml of cell culture s...

example 3

[0025] Example 3: Preparation of tissue engineered microcartilage tissue

[0026] 1. Preparation of PLGA microcarriers: Reference [Jean-Manuel Péan et al. NGF release from poly(D, L-lactide-co-glycolide) microspheres.Effect of someformulation parameters on encapsulated NGF stability. Journal of Controlled Release. 1998 56(4 ): 175-187] Prepare PLGA microcarriers, screen the microcarriers with a particle size of 150 μm-250 μm, sterilize them with cobalt 60 and save them for later use.

[0027] 2. Preparation of tissue engineered microparticle cartilage tissue: under aseptic conditions, soak 20 ml of cell culture medium (FAD culture medium containing 10% fetal bovine serum, plus 5 ml of 2 mg / ml polylysine solution) 1 gram of PLGA microcarrier for 24 hours to make it fully swell; centrifuge to remove the supernatant, and then dry it naturally under aseptic conditions.

[0028] will contain 3 x 10 5 10ml of chondrocyte cell culture medium per ml was mixed with microcarriers, and...

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Abstract

A tissue engineering particle tissue and preparing process is characterized in that the tissue engineering particle tissue which is formed by cells to be compounded on micro-carriers comprises larger particle tissues which are formed by mutual connection of a plurality of particle tissues. The invention uses the tissue engineering technology to culture highly active tissue engineering particle tissues to implant in the body for repairing defected tissues and organs. The invention has a good cell growth state and higher cell activity, and can clinically solve the difficulties of less donor tissues and large repairing area, and the micro-carriers can finally be degraded, after the body is cured, no influences can be caused to the body. In the clinical treatment, the invention can be used as a transplant for repairing large area tissue defection and as filling material for repairing body depressed deformation, and can be transplanted to wound surfaces. The invention can also be used by injection, and is extensive in application range and convenient in utilization.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a tissue engineered microparticle tissue and a preparation method thereof. Background technique [0002] In the field of surgical repair, the problem of insufficient skin source is often encountered, especially the large-scale skin defects caused by large-scale deep burns bring difficulties to surgery and treatment. Micro-skin technology is a commonly used autologous skin transplantation technique in clinical practice of burns. When the autologous skin is seriously insufficient, the remaining limited skin source of the patient is used to physically make it 0.5mm 3 ~1mm 3 The skin fragments are scattered and transplanted on the wound at a certain interval, and the proliferation ability of skin cells is used to form skin islands and gradually connect with each other to heal the wound; its advantage is that it can maximize the use of i...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/36A61L27/60
Inventor 金岩张勇杰
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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