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Methods for increasing isolation yields of cellular products

A product and cell technology, which is applied in the field of increasing the separation yield of cell products, can solve problems such as the impact on the yield of functional islets, and achieve the effect of increasing quantity and quality

Active Publication Date: 2013-08-21
LIFELINE SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at this time, donor pancreas harvesting for islet isolation and transplantation has not been widely practiced, in part because of concerns about the impact of postmortem ischemia on the yield of functional islets.

Method used

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  • Methods for increasing isolation yields of cellular products
  • Methods for increasing isolation yields of cellular products
  • Methods for increasing isolation yields of cellular products

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Embodiment Construction

[0119] Uniform perfusion can be achieved along the head of the pancreas including the duodenal segment. Can be ruled out from the pancreaticoduodenal artery ( figure 1 Leakage of small blood vessels branching from the two vascular loops surrounding the head of the pancreas, which in pigs is located between the head of the pancreas and the duodenum, enables equal perfusion of the head and neck of the pancreas. In addition, the opening of the pancreatic duct into the duodenum can be preserved. This procedure greatly facilitates cannulation of the pancreatic duct by avoiding the difficulties encountered in distinguishing the retracted catheter from the cannula, as well as preserving early duct branches. Preservation of early ductal branches may be necessary to ensure intact organ expansion for glandular digestion and islet isolation. It may be important to identify and tie all exposed vessels on the hepatic and gastroduodenal sides of the pancreas. Prior to perfusion, it may ...

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Abstract

Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a donor tissue having desired cells and undesired cells, perfusing the donor tissue with a perfusion solution, developing edema during perfusion of the donor tissue to form a swelled tissue, and separating the desired cells from undesired cellular material to obtain a cellular product. The methods may also include disrupting the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may result in an increased yield of cellular product that retains sufficient functional integrity to be useful as a transplantation resource.

Description

[0001] Cross References to Related Applications [0002] This non-provisional application claims priority to US Provisional Patent Application Serial No. 61 / 365,103, filed July 16, 2010. The content of this prior application is incorporated herein by reference in its entirety. Background technique [0003] Modern medicine, cell therapy, regenerative medicine and tissue engineering all involve the acquisition, expansion, transformation and replantation of living cells and tissues. A prime example is the transplantation of isolated islets of Langerhans for the treatment of type I (insulin dependent) diabetes. Since the first experimental attempts to ameliorate type 1 diabetes by transplantation of allogeneic donor islets, the need for improved methods of islet extraction from donor pancreas has been a challenge in the field. In fact, there are many global efforts to further develop the concept of treating type 1 diabetes by transplanting islets, but the clinical application o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/39A01N1/02C12N5/071
CPCA01N1/0247C12N5/0676
Inventor M·J·泰勒S·C·巴伊库D·克拉维茨
Owner LIFELINE SCI
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