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Alga microorganism fertilizer agent and preparation method thereof

A technology of microbial fertilizer and seaweed, applied in the direction of organic fertilizers, etc., can solve the problems such as the questionable effect of filtering out nutrients in the filter cake, time-consuming and labor-intensive, tedious raw material processing and preparation process, etc.

Active Publication Date: 2013-02-13
日照巴斯德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the raw material processing and preparation process is cumbersome, time-consuming and labor-intensive, and the filtering effect of nutrients in the filter cake is also open to question.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The seaweed microbial fertilizer water preparation described in the present embodiment is prepared through the following steps:

[0051] S01, the preserved Rhodopseudomonas palustris, Streptomyces flavinus, and Azotobacter ureus were respectively expanded and cultivated at 33°C, 30°C, and 29°C, and then the obtained strains were mixed according to the weight ratio of 2:2:1 The number is mixed to obtain the biological bacteria liquid, which is set aside;

[0052] The formula of the rhodopseudomonas palustris culture medium is: yeast powder 3.0g, peptone 3.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g, calcium chloride 0.3g, distilled water 1000mL. Adjust the pH value to 6.8-7.0, and autoclave the prepared medium at 121°C for 20 minutes.

[0053] The Streptomyces luteus culture medium formula is: soluble starch 2.0g, potassium nitrate 0.1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.05g, ferrous sulfate 0.001g, aga...

Embodiment 2

[0061] The seaweed microbial fertilizer water preparation described in the present embodiment is prepared through the following steps:

[0062] S01, the preserved Rhodopseudomonas palustris, Streptomyces flavinus, and Azotobacter ureus are respectively expanded and cultured, and then the resulting strains are mixed according to the weight ratio of 2:2:1 to obtain a biological bacterial liquid, which is set aside ;

[0063] The formula of the rhodopseudomonas palustris culture medium is: yeast powder 3.0g, peptone 3.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g, calcium chloride 0.3g, distilled water 1000mL. Adjust the pH value to 6.8-7.0, and autoclave the prepared medium at 121°C for 20 minutes.

[0064] The Streptomyces luteus culture medium formula is: soluble starch 2.0g, potassium nitrate 0.1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.05g, ferrous sulfate 0.001g, agar 2g, water 100ml. First put the starch in a b...

Embodiment 3

[0073] The seaweed microbial fertilizer water preparation described in the present embodiment is prepared through the following steps:

[0074] S01, the preserved Rhodopseudomonas palustris and Rhodospirillum chrysanthemum, Streptomyces flavinus and Frankia, Azotobacter rotundum and Bayerrinckia indica were respectively carried out to expand culture, and then the obtained The bacterial classification is mixed according to the parts by weight of 2:4:1 to obtain the biological bacterial liquid, for subsequent use;

[0075] The formulation of the culture medium for Rhodopseudomonas palustris and Rhodospirillum chrysogenum is: 0.5 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 3 mL of 95% alcohol, 2 g of yeast extract juice, and 1000 mL of sterilized sea water.

[0076] The formula of the Streptomyces luteus and Frankia culture medium is: soluble starch 2.0g, potassium nitrate 0.1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g, magnesium sulfate (M...

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Abstract

The invention relates to an alga microorganism fertilizer agent and a preparation method thereof and belongs to the technical field of aquiculture and the like. The alga microorganism fertilizer agent is fermented in such a way that monosodium glutamate waste liquid, alga paste, photosynthetic bacteria, actinomycetes, azotobacters and bacillus subtilis are compounded, and meanwhile, chitosan, chitosan oligosaccharide, microelements and molasses are added according to appropriate parts. The alga microorganism fertilizer agent is formed in such a way that the monosodium glutamate waste liquid is adopted for fermentation, and the raw materials preparation is simple; high-content compound bacteria are contained (the content of the bacillus subtilis is larger than three hundred million / mL, and the total content of the photosynthetic bacteria, the actinomycetes and the azotobacters is larger than one hundred million / mL) and the fertilizing effect is obvious; and the alga microorganism fertilizer agent also has a function of purifying water body, has a wide application range, and is suitable for sea-water and fresh-water cultivation.

Description

technical field [0001] The invention relates to a seaweed microbial fertilizer water preparation and a preparation method thereof, belonging to the technical field of aquaculture and the like. Background technique [0002] In the current economic development, the aquaculture industry has formed an industrial scale in our country and occupies an important position in the national economy. In the whole process of aquaculture industry, the fertilization of aquaculture water (also known as fertilizer water) occupies a very important position, which directly affects the aquaculture output. Generally, fertilizer and aquatic products will be used every 10-15 days or according to changes in water quality, especially after "water transfer" occurs, a large number of phytoplankton will die, seriously affecting photosynthesis, and the pond will suffer from hypoxia, causing the death of aquatic animals , At this time, it is necessary to use fertilizer and water products to multiply the ...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 战培林桑俊玲李作梅迟玉鹏
Owner 日照巴斯德生物科技有限公司
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