Method for producing efficient composite microbial preparation by livestock and poultry liquid dung
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A technology of compound microbial agent and livestock and poultry manure, which is applied in the field of environmental microbiology and agricultural science, can solve the problems of livestock and poultry manure polluting the environment, and achieve the effect of solving pollution
Inactive Publication Date: 2009-08-12
SHENYANG HUIZE BIOTIC PROD
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Problems solved by technology
[0005] The technical problem to be solved by the present invention is: according to the principles of microbiology and microbial ecology, the present invention provides a method for producing high-efficiency composite microbial bacterial agents using livestock and poult
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Embodiment 1
[0042] Step 1: Aseptically inoculate Rhodopseudomonas palustris and Rhodobacter sphaeroides in 1L of photosynthetic bacteria enrichment medium, mix and culture at 3000Lux, 27°C for 7 days, and expand the culture under the same conditions 1000 kilograms of photosynthetic bacteria liquid, spare;
[0043] Step 2: Aseptically inoculate Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum in 1L MRS medium, mix and cultivate at 27°C for 36 hours, and then expand and cultivate 100 kg of lactic acid bacteria liquid under the same conditions ,spare;
[0044] Step 3: Aseptically inoculate brewer's yeast, Candida tropicalis and Candida utilis in 1L of PDA medium, mix and culture at 27°C for 48 hours, and expand and culture 100 kg of yeast under the same conditions Bacteria solution, spare;
[0045] Step 4: Aseptically inoculate Bacillus subtilis, Bacillus megaterium and Bacillus colioids in 1L of beef extract peptone medium, mix and culture at 37°C for 24 hours, and...
Embodiment 2
[0054] Step 1: Aseptically inoculate Rhodopseudomonas palustris and Rhodobacter sphaeroides in 1L of photosynthetic bacteria enrichment medium, mix and culture at 3000Lux, 27°C for 7 days, and expand the culture under the same conditions 2000 kilograms of photosynthetic bacteria liquid, spare;
[0055] Step 2: Aseptically inoculate Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum in 1L MRS medium, mix and culture at 27°C for 36 hours, and then expand and cultivate 200 kg of lactic acid bacteria liquid under the same conditions ,spare;
[0056] Step 3: Aseptically inoculate brewer's yeast, Candida tropicalis and Candida utilis in 1L of PDA medium, mix and culture at 27°C for 48 hours, and expand and cultivate 200 kg of yeast under the same conditions Bacteria solution, spare;
[0057] Step 4: Aseptically inoculate Bacillus subtilis, Bacillus megaterium and Bacillus colioids in 1L beef extract peptone medium, mix and culture at 37°C for 24 hours, and th...
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Abstract
The invention relates to a method for producing a high-efficiency composite microbial agent by using livestock liquid dung, and belongs to the technical field of environmental microbiology and agricultural science. The method comprises the following steps: diluting livestock liquid dung or livestock feces with water; after hydrolysis and acidification treatment, performing standing and settlement for desilting; mixing a supernatant obtained with a photosynthetic bacterial solution for anaerobic culture; using the mixed solution as a base liquid and stepwise adding lactobacilli, saccharomyces, bacillus thuringienesis and actinomycete into the base liquid in a proportion; and after anaerobic culture and aerobic treatment, finally filling the mixture into a biological fluidized bed for further treatment to obtain the high-efficiency composite microbial agent. The microbial agent is rich in the photosynthetic bacteria, the lactobacilli, the saccharomyces, the actinomycete, the bacillus thuringienesis, azotobacter, nitrobacteria, denitrifying bacteria and other beneficial organisms, and be widely used in agriculture, livestock breeding, fresh water aquaculture, organic sewage treatment, microbial deodorization, and other aspects. The technology is suitable to be used by various livestock farms (raisers) for liquid dung treatment.
Description
technical field [0001] The invention relates to a production method of a high-efficiency composite microbial agent, in particular to a method for producing a high-efficiency composite microbial agent by using livestock and poultry manure, and belongs to the technical fields of environmental microbiology and agricultural science. Background technique [0002] With the rapid development of my country's national economy, GDP has maintained a high-speed growth trend for several years in a row. At the same time, the rapid economic development has also led to varying degrees of damage to the ecological environment, especially the pollution of water resources. One of the important The reason is the pollution from livestock and poultry manure. According to statistics, the annual excrement of livestock and poultry in my country is about 140 million tons in terms of dry matter, and the total amount of feces is several billion tons, and more than 80% of them are directly discharged with...
Claims
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