Plant composition with effects of controlling oil and removing acnes as well as preparation method and application of plant composition

Abstract

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to a plant composition with oil control and acne removal effects and a preparation method and application thereof. The invention provides an oil-controlling and acne-removing plant composition. The oil-controlling and acne-removing plant composition is prepared from a rheum palmatum extract, a scutellaria baicalensis extract, a radix sophorae flavescentis extract, a safflower extract, a campsis grandiflora extract, a chrysanthemum extract, a cockscomb extract and a rose extract in a specific proportion. Through synergistic compatibility of eight plants, the plant composition can effectively inhibit grease synthesis, inflammatory factor expression and propionibacterium acnes growth, so that good oil control and acne removal effects are achieved.

Description

Technical Field

[0001] This application belongs to the field of cosmetic raw material technology, specifically relating to plant compositions with oil-controlling and acne-reducing effects, their preparation methods, and applications. Background Technology

[0002] With increasing life pressures and changing dietary habits, oily skin has gradually become a problem plaguing many people, leading to a rise in acne and other skin conditions. Acne is a common chronic inflammatory disease of the hair follicles and sebaceous glands. Its main pathogenic mechanisms include excessive sebum secretion, abnormal differentiation and blockage of keratinocytes at the hair follicle opening, excessive proliferation of Propionibacterium acnes, and the resulting inflammatory response. For a long time, the cosmetics and dermatology fields have mainly relied on chemically synthesized ingredients to address these problems, such as salicylic acid to unclog pores, benzoyl peroxide for sterilization, and retinoic acid and its derivatives to regulate keratinization. Although these ingredients have proven effective, in practice, they are often accompanied by significant skin irritation, dryness, flaking, and redness.

[0003] Existing oil-control and acne-removing products primarily focus on "post-treatment," meaning intensive treatment after acne has formed. However, they pay insufficient attention to preventing acne, regulating the skin's microecological balance, and repairing damaged skin barriers. Furthermore, as consumers increasingly favor products with superior safety, gentleness, and naturally derived ingredients, developing innovative solutions that combine excellent efficacy with a pleasant skin feel has become an urgent market need.

[0004] Therefore, it is particularly important to develop oil-controlling and acne-removing compositions with plant-based active ingredients as the core. Summary of the Invention

[0005] Based on this, one embodiment of this application provides a plant composition with oil-controlling and acne-removing effects, its preparation method, and its application.

[0006] This application provides a plant-based composition with oil-controlling and acne-removing effects, wherein the composition comprises the following components in parts by weight:

[0007] 1-50 parts of Rheum palmatum extract, 1-50 parts of Scutellaria baicalensis extract, 1-50 parts of Sophora flavescens extract, 1-50 parts of Carthamus tinctorius extract, 1-50 parts of Campsis grandiflora extract, 1-30 parts of Chrysanthemum extract, 1-30 parts of Celosia cristata extract, and 1-30 parts of Rosa rugosa extract.

[0008] In some embodiments, the rhubarb extract comprises an aqueous or alcoholic extract of rhubarb.

[0009] In some embodiments, the Scutellaria baicalensis extract includes an aqueous extract or an alcoholic extract of Scutellaria baicalensis.

[0010] In some embodiments, the Sophora flavescens extract includes an aqueous extract or an alcoholic extract of Sophora flavescens.

[0011] In some embodiments, the safflower extract includes an aqueous or alcoholic extract of safflower.

[0012] In some embodiments, the trumpet vine extract includes a water extract or an alcohol extract of the trumpet vine.

[0013] In some embodiments, the chrysanthemum extract includes an aqueous or alcoholic extract of chrysanthemum.

[0014] In some embodiments, the cockscomb flower extract includes a water extract or an alcohol extract of cockscomb flower.

[0015] In some embodiments, the rose extract includes a water extract or an alcohol extract of roses.

[0016] This application also provides a method for preparing the aforementioned plant composition with oil-controlling and acne-reducing effects, comprising:

[0017] A plant composition with oil-controlling and acne-reducing effects was prepared using the extracts of Rheum palmatum, Scutellaria baicalensis, Sophora flavescens, Carthamus tinctorius, Campsis grandiflora, Chrysanthemum morifolium, Celosia cristata, and Rosa rugosa.

[0018] In some embodiments, the preparation method includes: crushing and mixing rhubarb, scutellaria, sophora flavescens, safflower, trumpet creeper, chrysanthemum, cockscomb, and rose to prepare a preparatory material;

[0019] The prepared material is subjected to high-pressure microfluidic extraction after adding an extraction solvent to prepare an extract.

[0020] The extract is concentrated under reduced pressure to prepare a concentrate; the concentrate is mixed with a stabilizer and then reconstituted, centrifuged, and the supernatant is collected; and the supernatant is subjected to membrane filtration to prepare a plant composition with oil-controlling and acne-removing effects.

[0021] In some embodiments, the extraction solvent includes one or more of water, ethanol, or an aqueous solution of ethanol;

[0022] In some embodiments, the mass-to-volume ratio of the preparative material to the extraction solvent is 1:(10-30)g / mL.

[0023] In some embodiments, the conditions for high-pressure microjets extraction include: a pressure of 80 MPa - 120 MPa, an extraction temperature of 20°C - 60°C, and an extraction time of 1 h - 3 h.

[0024] In some embodiments, the conditions for vacuum concentration include: a pressure of 2 kPa to 20 kPa and a temperature of 40°C to 60°C.

[0025] In some embodiments, the centrifugation speed is 5000 r / min - 20000 r / min.

[0026] In some embodiments, the stabilizer includes one or more of butanediol, dipropylene glycol, glycerol, and 1,3-propanediol.

[0027] In some embodiments, the filter membrane used in membrane filtration includes one or more of microfiltration membranes, ultrafiltration membranes, and nanofiltration membranes.

[0028] In some embodiments, the microfiltration membrane has a pore size of 0.1 μm-3 μm, the ultrafiltration membrane has a pore size of 10 nm-50 nm, and the nanofiltration membrane has a pore size of 100 Da-1000 Da.

[0029] This application also provides a product with oil-controlling and acne-removing effects, including the plant composition with oil-controlling and acne-removing effects described above or the plant composition with oil-controlling and acne-removing effects prepared by the preparation method described above.

[0030] In some embodiments, the product includes the plant composition and pharmaceutically acceptable excipients.

[0031] In some embodiments, the amount of the plant composition with oil-controlling and acne-reducing effects added to the product is 0.1%-5% by mass.

[0032] In some embodiments, the product includes the plant composition as well as cosmetic excipients.

[0033] In some embodiments, the cosmetically acceptable excipients include one or more of fillers, diluents, lubricants, wetting agents, and adhesives.

[0034] In some embodiments, the product having oil-controlling and acne-reducing effects may be in one or more of the following forms: cream, liquid, lotion, and foam.

[0035] In some embodiments, the product with oil-controlling and acne-reducing effects includes one or more of toners, serums, creams, masks, and gels.

[0036] This application provides a plant-based composition for oil control and acne removal, comprising extracts of Rheum palmatum, Scutellaria baicalensis, Sophora flavescens, Carthamus tinctorius, Campsis grandiflora, Chrysanthemum morifolium, Celosia cristata, and Rosa rugosa in specific proportions. This plant composition, through the synergistic combination of eight plants, effectively inhibits sebum synthesis, suppresses the expression of inflammatory factors, and inhibits the growth of Propionibacterium acnes, thereby exerting excellent oil control and acne removal effects. Rheum palmatum, Scutellaria baicalensis, and Sophora flavescens serve as the principal herbs, clearing heat from the lungs and stomach at its source, drying dampness and promoting diuresis, cutting off the root cause of sebum and inflammation; Carthamus tinctorius and Campsis grandiflora serve as assistant herbs, clearing heat while cooling the blood and promoting blood circulation, resolving blood stasis caused by heat; Chrysanthemum morifolium and Celosia cristata serve as adjuvant herbs, clearing heat and detoxifying, astringing and promoting tissue regeneration for existing acne; Rosa rugosa serves as the guiding herb, directing the medicine upwards to the skin and harmonizing the effects of the other herbs. This comprehensive traditional Chinese medicine formulation system, integrating "clearing the source, unblocking, treating the symptoms, and guiding," achieves a combined effect of oil control, anti-inflammation, acne removal, and scar removal. Attached Figure Description

[0037] To more clearly illustrate the technical solutions in the embodiments of this application and to more completely understand this application and its beneficial effects, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0038] Figure 1 This is a Venn diagram of acne and sebum targets versus potential compound targets provided in one embodiment of this application.

[0039] Figure 2 Protein-protein interaction network analysis of the composition provided in one embodiment of this application with skin acne and sebum overlap targets;

[0040] Figure 3 This is a functional enrichment map of the core target GO provided in an embodiment of this application;

[0041] Figure 4 This is an enrichment map of the KEGG pathway, a core target, provided in an embodiment of this application.

[0042] Compound Target; Acne Target; Enrichment score; Cellular response to lipid; Intracellular receptor signaling pathway; Rapid development; Regulation of mRNA transcription; Regulation of mRNA metabolic process; Transcription regulator complex; Spindle; Centrosome; Receptor complex; RNA polymerase II transcription regulator complex; Ligand-modulated transcription factor binding; DNA-binding transcription factor binding; Biological process; Cellular component; Molecular function. Detailed Implementation

[0043] The present application will be further described in detail below with reference to the embodiments and examples. It should be understood that these embodiments and examples are for illustrative purposes only and are not intended to limit the scope of the present application. The purpose of providing these embodiments and examples is to enable a more thorough and comprehensive understanding of the disclosure of the present application. It should also be understood that the present application can be implemented in many different forms and is not limited to the embodiments and examples described herein. Those skilled in the art can make various modifications or alterations without departing from the spirit of the present application, and the equivalent forms obtained also fall within the protection scope of the present application. Furthermore, numerous specific details are set forth in the following description to provide a fuller understanding of the present application. It should be understood that the present application can be implemented without one or more of these details.

[0044] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0045] Unless otherwise stated or in case of contradiction, the terms or phrases used herein shall have the following meanings:

[0046] The terms "and / or," "or / and," and "and / or" as used herein include any one of two or more of the related listed items, as well as any and all combinations of the related listed items. These arbitrary and all combinations include any two related listed items, any more related listed items, or a combination of all related listed items. It should be noted that when at least three items are connected by at least two conjunctions selected from "and / or," "or / and," and "and / or," it should be understood that in this application, the technical solution undoubtedly includes technical solutions connected by "logical AND," and also undoubtedly includes technical solutions connected by "logical OR." For example, "A and / or B" includes three parallel solutions: A, B, and A+B. For example, the technical solution of "A, and / or, B, and / or, C, and / or, D" includes any one of A, B, C, and D (that is, a technical solution that is connected by "logical OR"), as well as any and all combinations of A, B, C, and D, that is, combinations of any two or three of A, B, C, and D, and also combinations of all four of A, B, C, and D (that is, a technical solution that is connected by "logical AND").

[0047] In this application, the terms "multiple", "various", "multiple times", "multi-dimensional", etc., unless otherwise specified, refer to a quantity greater than or equal to 2. For example, "one or more" means one or more than or equal to two.

[0048] The terms “combinations of,” “any combination of,” and “any combination of” used in this article include all suitable combinations of any two or more of the listed items.

[0049] In this document, the term "suitable" as used in phrases such as "suitable combination," "suitable method," and "any suitable method" refers to the ability to implement the technical solution of this application, solve the technical problem of this application, and achieve the expected technical effect of this application.

[0050] In this application, terms such as "further," "even further," and "particularly" are used to describe purposes and indicate differences in content, but should not be construed as limiting the scope of protection of this application.

[0051] In this application, "optionally," "optionally," and "optional" mean that something is optional, that is, it means that it is selected from either "with" or "without." If there are multiple "optional" entries in a technical solution, unless otherwise specified, and there are no contradictions or mutual constraints, each "optional" entry shall be independent.

[0052] In this application, the technical features described in an open-ended manner include both closed technical solutions composed of the listed features and open technical solutions composed of the listed features.

[0053] In this application, numerical intervals (i.e., numerical ranges) are involved. Unless otherwise specified, the selected numerical distributions within the aforementioned numerical intervals are considered continuous and include the two endpoints (i.e., the minimum and maximum values) of the numerical range, as well as every value between these two endpoints. Unless otherwise specified, when a numerical interval refers only to integers within that interval, it includes the two endpoint integers of the numerical range, as well as every integer between the two endpoints. In this document, this is equivalent to directly listing every integer. For example, if t is an integer selected from 1 to 10, it means that t is any integer selected from the group of integers consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10. Furthermore, when multiple ranges are provided to describe features or characteristics, these ranges can be merged. In other words, unless otherwise specified, the ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0054] Unless otherwise specified, the temperature parameters in this application are permitted to be either constant-temperature treatment or variations within a certain temperature range. It should be understood that the constant-temperature treatment allows temperature fluctuations within the precision range of the instrument control, such as ±5℃, ±4℃, ±3℃, ±2℃, or ±1℃.

[0055] In this application, % (w / w) and wt% both represent weight percentage, % (v / v) refers to volume percentage, and % (w / v) refers to mass-volume percentage.

[0056] All references to documents mentioned in this application are incorporated herein by reference as if each document were individually incorporated herein by reference. Unless they conflict with the inventive purpose and / or technical solution of this application, all cited documents are incorporated herein by reference in their entirety and for all purposes. When citing documents in this application, the definitions of relevant technical features, terms, nouns, phrases, etc., are also incorporated herein by reference. When citing documents in this application, examples and preferred embodiments of the cited technical features may also be incorporated herein by reference, but only to the extent that they enable the implementation of this application. It should be understood that when the cited content conflicts with the description in this application, this application shall prevail or modifications shall be made adaptably to the description in this application.

[0057] The term "high-pressure microjets extraction process" refers to a modern, highly efficient extraction technology. Its principle involves forcing material through a micron-sized nozzle under ultra-high pressure (50-150 MPa), generating strong shearing forces, cavitation effects, and impact forces, thereby effectively breaking down plant cell walls and releasing intracellular active ingredients. Compared to traditional extraction methods, high-pressure microjets extraction offers advantages such as high extraction efficiency, short extraction time, low energy consumption, and complete preservation of active ingredients. In this invention, the process can be carried out at relatively low temperatures (≤60℃), effectively avoiding the decomposition of heat-sensitive active ingredients (such as flavonoids and polyphenols) and ensuring the bioactivity of the plant composition.

[0058] The term "network pharmacology approach" refers to a new discipline based on systems biology theory. It involves analyzing the networks of biological systems to select specific signaling nodes for multi-target drug molecule design. In this invention, the network pharmacology approach mainly includes four steps: 1) screening plant active ingredients (OB≥30%, DL≥0.18) using the TCMSP database; 2) predicting potential targets of active ingredients using the SwissTarget Prediction platform; 3) constructing a protein-protein interaction network using the String database; and 4) performing GO functional annotation analysis and KEGG pathway enrichment analysis using the Metascape database. This method can systematically elucidate the mechanism of action of traditional Chinese medicine compound prescriptions, providing a scientific basis for the rational compatibility of plant compositions.

[0059] AKT1, TP53, TNF, IL6, PPARG, and ESR1 targets: These are the core targets identified in this application through network pharmacology analysis. AKT1 (protein kinase B) participates in the regulation of cell proliferation, survival, and metabolism; TP53 (tumor suppressor protein p53) plays an important role in cell cycle regulation and DNA repair; TNF (tumor necrosis factor) is a major pro-inflammatory cytokine; IL6 (interleukin-6) participates in inflammatory responses and immune regulation; PPARG (peroxisome proliferator-activated receptor γ) regulates adipocyte differentiation and lipid metabolism; and ESR1 (estrogen receptor α) participates in hormone signal transduction. These targets are closely related to sebum secretion, inflammatory responses, and cell proliferation, and are key points of action for the plant composition to exert its oil-controlling and acne-reducing effects.

[0060] One application provides a plant-based composition with oil-controlling and acne-reducing effects, comprising the following components by weight:

[0061] 1-50 parts of Rheum palmatum extract, 1-50 parts of Scutellaria baicalensis extract, 1-50 parts of Sophora flavescens extract, 1-50 parts of Carthamus tinctorius extract, 1-50 parts of Campsis grandiflora extract, 1-30 parts of Chrysanthemum extract, 1-30 parts of Celosia cristata extract, and 1-30 parts of Rosa rugosa extract.

[0062] This application provides a plant-based composition for oil control and acne removal, comprising rhubarb, scutellaria, sophora flavescens, safflower, trumpet creeper, chrysanthemum, cockscomb, and rose. This plant composition, through the synergistic combination of eight plants, effectively inhibits sebum synthesis, suppresses the expression of inflammatory factors, and inhibits the growth of Propionibacterium acnes, thereby exerting excellent oil control and acne removal effects. Rhubarb, scutellaria, and sophora flavescens serve as the principal herbs, clearing away excess heat in the lungs and stomach at its source, drying dampness and promoting diuresis, cutting off the root cause of sebum and inflammation; safflower and trumpet creeper serve as assistant herbs, clearing heat while cooling the blood and promoting blood circulation, resolving blood stasis caused by heat; chrysanthemum and cockscomb serve as adjuvant herbs, clearing heat and detoxifying, astringing and promoting tissue regeneration for existing acne; rose serves as the guiding herb, directing the medicine upwards to the skin and harmonizing the effects of the other herbs. This complete traditional Chinese medicine formulation system, integrating "clearing the source, unblocking, treating the symptoms, and guiding," achieves a comprehensive effect of oil control, anti-inflammation, acne removal, and scar removal.

[0063] Specifically, the rhubarb extract can be selected from any number of parts: 10, 20, 30, 40, or 50; the scutellaria baicalensis extract can be selected from any number of parts: 10, 20, 30, 40, or 50; the sophora flavescens extract can be selected from any number of parts: 10, 20, 30, 40, or 50; the safflower extract can be selected from any number of parts: 10, 20, 30, 40, or 50; and the lingzhi extract... The extract of *Celosia argentea* can be selected from any number of 10, 20, 30, 40, or 50 parts; the extract of *Chrysanthemum morifolium* can be selected from any number of 5, 10, 15, 20, 25, or 30 parts; the extract of *Celosia cristata* can be selected from any number of 5, 10, 15, 20, 25, or 30 parts; and the extract of *Rosa rugosa* can be selected from any number of 5, 10, 15, 20, 25, or 30 parts.

[0064] In some embodiments, the rhubarb extract comprises an aqueous or alcoholic extract of rhubarb.

[0065] In some embodiments, the Scutellaria baicalensis extract includes an aqueous extract or an alcoholic extract of Scutellaria baicalensis.

[0066] In some embodiments, the Sophora flavescens extract includes an aqueous extract or an alcoholic extract of Sophora flavescens.

[0067] In some embodiments, the safflower extract includes an aqueous or alcoholic extract of safflower.

[0068] In some embodiments, the trumpet vine extract includes a water extract or an alcohol extract of the trumpet vine.

[0069] In some embodiments, the chrysanthemum extract includes an aqueous or alcoholic extract of chrysanthemum.

[0070] In some embodiments, the cockscomb flower extract includes a water extract or an alcohol extract of cockscomb flower.

[0071] In some embodiments, the rose extract includes a water extract or an alcohol extract of roses.

[0072] The above-mentioned plant compositions were screened using a network pharmacology approach, which includes: screening active ingredients through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), constructing a protein-protein interaction network, and performing core target enrichment analysis. The core targets screened using this method include AKT1, TP53, TNF, IL6, PPARG, and ESR1, which are closely related to skin acne and sebum, providing a scientific basis for the oil-controlling and acne-reducing efficacy of the plant compositions.

[0073] This application also provides a method for preparing the aforementioned plant composition with oil-controlling and acne-reducing effects, comprising:

[0074] A plant composition with oil-controlling and acne-removing effects was prepared using the Scutellaria baicalensis extract, Sophora flavescens extract, Carthamus tinctorius extract, Campsis grandiflora extract, Chrysanthemum extract, Celosia cristata extract, and Rosa rugosa extract.

[0075] The preparation method of the plant composition with oil-controlling and acne-removing effects described in some embodiments includes: crushing and mixing rhubarb, scutellaria baicalensis, sophora flavescens, safflower, trumpet creeper, chrysanthemum, cockscomb and rose to prepare a preparatory material;

[0076] The prepared material is subjected to high-pressure microfluidic extraction after adding an extraction solvent to prepare an extract.

[0077] The extract is concentrated under reduced pressure to prepare a concentrated solution; the concentrated solution is mixed with a stabilizer and reconstituted, then centrifuged; the supernatant is collected and the supernatant is subjected to membrane filtration to prepare a plant composition with oil-controlling and acne-removing effects.

[0078] In some embodiments, the extraction solvent includes one or more of water, ethanol, or an aqueous solution of ethanol.

[0079] In some embodiments, the mass-to-volume ratio of the preparative material to the extraction solvent is 1:(10-30). Specifically, the mass-to-volume ratio of the preparative material to the extraction solvent is 1:10, 1:15, 1:20, 1:25, or 1:30.

[0080] In some embodiments, the conditions for high-pressure microjets extraction include: a pressure of 80 MPa-120 MPa, an extraction temperature of 20℃-60℃, and an extraction time of 1h-3h. Specifically, the extraction pressure can be selected from any value among 80 MPa, 100 MPa, and 120 MPa; the extraction temperature can be selected from any value among 20℃, 30℃, 40℃, 50℃, and 60℃; and the extraction time can be selected from any value among 1h, 2h, and 3h.

[0081] In some embodiments, the conditions for vacuum concentration include: a pressure of 2 kPa-20 kPa and a temperature of 40°C-60°C; for example, pressures of 2 kPa, 4 kPa, 6 kPa, 8 kPa, 10 kPa, 12 kPa, 14 kPa, 16 kPa, 18 kPa, or 20 kPa and any intermediate values; and temperatures of 40°C, 45°C, 50°C, 55°C, or 60°C and any intermediate values.

[0082] In some embodiments, the centrifugation speed is 5000 r / min - 20000 r / min; for example, the centrifugation speed is 5000 r / min, 10000 r / min, 15000 r / min or 20000 r / min and any value in between.

[0083] In some embodiments, the stabilizer includes one or more of butanediol, dipropylene glycol, glycerol, and 1,3-propanediol.

[0084] In some embodiments, the filter membrane used in membrane filtration includes one or more of microfiltration membranes, ultrafiltration membranes, and nanofiltration membranes.

[0085] The microfiltration membrane has a pore size of 0.1 μm-3 μm, the ultrafiltration membrane has a pore size of 10 nm-50 nm, and the nanofiltration membrane has a pore size of 100 Da-1000 Da. For example, the pore size of the microfiltration membrane can be 0.1 μm, 0.2 μm, 0.4 μm, 0.6 μm, 0.8 μm, 1.0 μm, 1.2 μm, 1.4 μm, 1.6 μm, 1.8 μm, 2.0 μm, 2.2 μm, 2.4 μm, 2.6 μm, 2.8 μm, or 3.0 μm, or any value in between.

[0086] Specifically, the preparation method of this application includes the following steps: raw material pretreatment, extraction and separation, and concentration and purification. This preparation method utilizes a high-pressure microfluidic extraction process, which generates strong shear force and cavitation effect under ultra-high pressure to break down plant cell walls, resulting in extremely high extraction efficiency. Furthermore, the extraction and purification temperature remains below 60℃ throughout the entire process, preventing the decomposition of heat-sensitive substances and better preserving the activity of the active ingredients.

[0087] This application also provides a product with oil-controlling and acne-removing effects, including the plant composition with oil-controlling and acne-removing effects described above or the plant composition with oil-controlling and acne-removing effects prepared by the preparation method described above.

[0088] In some embodiments, the product includes the plant composition and pharmaceutically acceptable excipients;

[0089] In some embodiments, the plant composition with oil-controlling and acne-reducing effects is added to the product at a mass concentration of 0.1%-5%. For example, the mass concentration of the plant composition with oil-controlling and acne-reducing effects in the product is 0.1%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, 4.5%, or 5.0%, or any value in between.

[0090] In some embodiments, the product includes the plant composition as well as cosmetic excipients.

[0091] In some embodiments, the cosmetically acceptable excipients include one or more of fillers, diluents, lubricants, wetting agents, and adhesives.

[0092] In some embodiments, the product having oil-controlling and acne-reducing effects may be in one or more of the following forms: cream, liquid, lotion, and foam.

[0093] In some embodiments, the product with oil-controlling and acne-reducing effects includes one or more of toners, serums, creams, masks, and gels.

[0094] The embodiments of this application will be described in detail below with reference to examples. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of this application. For experimental methods in the following embodiments where specific conditions are not specified, please refer to the guidelines given in this application, or follow experimental manuals or conventional conditions in the art, or follow the conditions recommended by the manufacturer, or refer to experimental methods known in the art.

[0095] In the specific embodiments described below, the measurement parameters involving raw material components may have slight deviations within the weighing accuracy range unless otherwise specified. Temperature and time parameters are subject to acceptable deviations due to instrument testing accuracy or operational precision.

[0096] It should be understood that in the various embodiments of this application, the order of the above-mentioned processes does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0097] Example 1

[0098] I. Network pharmacology analysis:

[0099] 1. Collection of potential active plant ingredients in oil-controlling and acne-removing formulas

[0100] Data retrieval and analysis were conducted using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Potential active ingredients from rhubarb, scutellaria baicalensis, sophora flavescens, safflower, trumpet creeper, chrysanthemum, cockscomb, and rose were collected and screened using a dual screening threshold of oral bioavailability (OB) ≥30% and drug-likeness (DL) ≥0.18.

[0101] 2. Obtain a dataset of target points related to skin oil control and acne treatment.

[0102] Using the SwissTarget Prediction platform, 141 potential targets for the active ingredient in the composition were identified through screening and integration. Simultaneously, 1707 targets related to acne and 294 targets related to sebum were retrieved and screened from the GeneCards database. Venn diagram analysis was used to compare the intersection of the three groups of targets, revealing 16 common targets between the potential active ingredient targets and acne and sebum-related skin targets (e.g., ...). Figure 1 (As shown).

[0103] 3. Construct a protein-protein interaction network to identify the key targets of the effective components of traditional Chinese medicine.

[0104] Intersecting target points were imported into the STRING database for analysis, and network topology analysis and visualization were performed using Cytoscape 3.9.1 software. For example... Figure 2As shown, by calculating the degree value and betweenness centrality of nodes using software, the importance of target points in the network was ranked. The top-ranked core targets were found to be AKT1, TP53, TNF, IL6, PPARG, and ESR1. These targets can be preliminarily identified as key targets for the regulation of skin acne and sebum by potential active ingredients for oil control and acne removal.

[0105] 4. Enrichment analysis of core targets

[0106] To systematically elucidate the molecular mechanisms related to the research objectives, functional enrichment analysis was performed on 16 co-expressed targets identified in the initial screening using the Metascape platform. The results showed that multiple Gene Ontology (GO) functional annotation entries were identified, which could be clearly divided into three modules according to functional category: biological processes (BP), cellular components (CC), and molecular functions (MF). To further focus on core functional information, the significance level of the enrichment results was used as the screening criterion, and the top 10 highly significant functional entries in the three categories of BP, CC, and MF were finally selected and visualized using charts (such as...). Figure 3 As shown, this visually displays each item and its enrichment level, providing key functional evidence for further in-depth analysis of molecular mechanisms.

[0107] KEGG pathway enrichment analysis results revealed (e.g.) Figure 4 As shown in the figure, the composition may regulate key signaling pathways through multiple targets, thereby clarifying the specific signaling pathways and potential mechanisms of action involved in its oil-controlling and acne-reducing effects. The study identified multiple significantly enriched signaling pathways (P<0.05), which affect excessive sebum secretion and abnormal follicular keratinization through multiple pathways, thereby jointly exerting the effects of oil control and acne reduction.

[0108] Example 1

[0109] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 20g of Rheum palmatum extract, 20g of Scutellaria baicalensis extract, 20g of Sophora flavescens extract, 20g of Carthamus tinctorius extract, 20g of Campsis grandiflora extract, 20g of Chrysanthemum extract, 20g of Celosia cristata extract, and 20g of Rosa rugosa extract.

[0110] Example 2

[0111] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 40g of Rheum palmatum extract, 30g of Scutellaria baicalensis extract, 20g of Sophora flavescens extract, 20g of Carthamus tinctorius extract, 10g of Campsis grandiflora extract, 10g of Chrysanthemum extract, 10g of Celosia cristata extract, and 20g of Rosa rugosa.

[0112] Example 3

[0113] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 10g of Rheum palmatum extract, 40g of Scutellaria baicalensis extract, 40g of Sophora flavescens extract, 10g of Carthamus tinctorius extract, 10g of Campsis grandiflora extract, 10g of Chrysanthemum extract, 20g of Celosia cristata extract, and 20g of Rosa rugosa extract.

[0114] Example 4

[0115] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 20g of Rheum palmatum extract, 10g of Scutellaria baicalensis extract, 10g of Sophora flavescens extract, 40g of Carthamus tinctorius extract, 40g of Campsis grandiflora extract, 20g of Chrysanthemum extract, 10g of Celosia cristata extract, and 10g of Rosa rugosa extract.

[0116] Example 5

[0117] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 20g of Rheum palmatum extract, 20g of Scutellaria baicalensis extract, 10g of Sophora flavescens extract, 10g of Carthamus tinctorius extract, 10g of Campsis grandiflora extract, 20g of Chrysanthemum extract, 40g of Celosia cristata extract, and 40g of Rosa rugosa extract.

[0118] Example 6

[0119] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 40g of Rheum palmatum extract, 10g of Scutellaria baicalensis extract, 10g of Sophora flavescens extract, 20g of Carthamus tinctorius extract, 20g of Campsis grandiflora extract, 40g of Chrysanthemum extract, 10g of Celosia cristata extract, and 10g of Rosa rugosa extract.

[0120] Example of preparation:

[0121] S1. Raw material pretreatment: Rhubarb palmatum, Scutellaria baicalensis, Sophora flavescens, Carthamus tinctorius, Campsis grandiflora, Chrysanthemum morifolium, Celosia cristata and Rosa rugosa are pulverized into powder using a pulverizer, passed through a 50-mesh sieve for later use, and the plant powder is accurately weighed and mixed evenly.

[0122] S2. Extraction and separation: The above-mentioned plant powder was added to a 50% ethanol-water (V / V) solution for extraction. The material-to-liquid ratio was 1:20 g / mL. High-pressure microjet extraction was performed at an extraction pressure of 100 MPa, an extraction temperature of 25℃, and an extraction time of 2 h. After the first extraction, the filtrate was collected by filtration. The residue was extracted a second time as described above. The supernatant was collected by filtration. The first and second supernatants were combined to obtain the extract.

[0123] S3. Concentration and Refining: The extract obtained in S2 was concentrated under reduced pressure at 10 kPa and 55°C until it was equivalent to the mass of plant powder. An equal mass of butanediol was added for redissolution, and the mixture was stirred evenly. The liquid was allowed to settle at low temperature for 15 hours. Then, it was centrifuged at high speed using a tubular centrifuge at 15,000 r / min. The supernatant was collected and filtered through a 0.22 μm pore size microfiltration membrane to obtain the final plant composition.

[0124] To demonstrate the effectiveness of this application, comparative experimental examples are provided below:

[0125] The preparation methods of any one of the oil-controlling and acne-removing plant compositions in Comparative Examples 1-6 are the same as those in the examples.

[0126] Comparative Example 1

[0127] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 40g of Sophora flavescens extract, 40g of Carthamus tinctorius extract, 20g of Campsis grandiflora extract, 20g of Chrysanthemum extract, 20g of Celosia cristata extract, and 20g of Rose extract.

[0128] Comparative Example 2

[0129] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 30g of Rheum palmatum extract, 30g of Scutellaria baicalensis extract, 40g of Campsis grandiflora extract, 20g of Chrysanthemum extract, 20g of Celosia cristata extract, and 20g of Rose extract.

[0130] Comparative Example 3

[0131] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 20g of Rheum palmatum extract, 20g of Scutellaria baicalensis extract, 20g of Sophora flavescens extract, 20g of Carthamus tinctorius extract, 40g of Celosia cristata extract, and 40g of Rosa rugosa extract.

[0132] Comparative Example 4

[0133] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 30g of Rheum palmatum extract, 20g of Scutellaria baicalensis extract, 20g of Sophora flavescens extract, 20g of Carthamus tinctorius extract, 40g of Campsis grandiflora extract, and 30g of Chrysanthemum extract.

[0134] Comparative Example 5

[0135] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 30g of Scutellaria baicalensis extract, 20g of Sophora flavescens extract, 20g of Carthamus tinctorius extract, 20g of Campsis grandiflora extract, 20g of Chrysanthemum extract, 20g of Celosia cristata extract, and 20g of Rosa rugosa extract.

[0136] Comparative Example 6

[0137] An oil-controlling and acne-removing plant composition is prepared by extracting the following components: 20g of Rheum palmatum extract, 20g of Carthamus tinctorius extract, 30g of Campsis grandiflora extract, 30g of Chrysanthemum extract, 30g of Celosia cristata extract, and 30g of Rose extract.

[0138] The aforementioned oil-controlling and acne-removing plant composition can be used to prepare oil-controlling and acne-removing cosmetics. The oil-controlling and acne-removing cosmetics are topical skin preparations made with the active ingredients of the aforementioned plant composition having oil-controlling and acne-removing effects, plus conventional pharmaceutical or cosmetic excipients or auxiliary ingredients. In specific use, the oil-controlling and acne-removing plant composition prepared in this application is directly added to the cosmetic matrix. The amount added can be adjusted according to actual needs, preferably 0.1wt%-5wt%.

[0139] To better illustrate the advantages of this application, the following are experimental results demonstrating the efficacy of the oil-controlling and acne-reducing plant-based composition provided in this application:

[0140] Test 1: IL-6 inhibition assay in a macrophage inflammation model

[0141] The plant compositions prepared in Examples 1-6 and Comparative Examples 1-6 were tested as follows:

[0142] 1. Cytotoxicity Assay: RAW264.7 cell suspension was adjusted to different concentrations and added to 96-well cell plates. The cells were incubated overnight at 37°C with 5% CO2 for assay. Test samples were diluted with cell culture medium to different concentrations and added to cell plates, then incubated at 37°C with 5% CO2 for 48 hours. The culture medium was discarded, the cells were washed twice with PBS, and MTT working solution was added for further incubation. After incubation, the culture medium was discarded, DMSO was added, and the mixture was shaken to mix. The absorbance was measured at 570 nm (with a background of 630 nm). Cytotoxicity was calculated based on the results, and, combined with cell morphology, the same concentration of samples showing no significant cytotoxicity was selected for further experiments.

[0143] 2. IL-6 Assay: RAW264.7 cell suspension was adjusted to a non-cytotoxic concentration and added to 96-well cell plates. The cells were incubated overnight at 37°C with 5% CO2 for detection. The test samples were diluted with LPS-containing cell culture medium to a non-cytotoxic concentration before addition. LPS-containing medium served as the negative control (NC), and LPS-free cell culture medium served as the blank control (BC). A positive control (PC, dexamethasone sodium phosphate) diluted with LPS-containing cell culture medium was used as the positive control. Cells were cultured for 20-24 hours after addition. After the assay, the supernatant was collected and analyzed according to the IL-6 ELISA kit procedure.

[0144] Result calculation and judgment:

[0145] Formula for calculating the downregulation rate of inflammatory factor IL-6: Downregulation rate = (1-T / NC) × 100%.

[0146] Wherein: T—mean value of inflammatory factor IL-6 in the experimental group; NC—mean value of inflammatory factor IL-6 in the negative control group.

[0147] Perform statistical and significance analysis on the data, calculate the P-value, P < 0.05 indicates a significant difference, otherwise there is no statistical difference.

[0148] When the IL-6 level in the negative control group was significantly higher than that in the blank control group (P < 0.05), the experiment was considered successful.

[0149] When the level of the inflammatory factor IL-6 in the sample group was lower than that in the negative control group, and the difference between the two was statistically significant (P < 0.05), the sample could be considered to have a soothing effect.

[0150] Table 1: Experimental results of plant composition on downregulation of inflammatory factor IL-6

[0151]

[0152] Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and 0.01 < P ≤ 0.05, denoted as "*"; 0.001 < P ≤ 0.01, denoted as "**"; 0.0001 < P ≤ 0.001, denoted as "***"; P ≤ 0.0001, denoted as "****".

[0153] The results of the experiments on the downregulation of the inflammatory factor IL-6 by the plant compositions of Examples 1-6 and Comparative Examples 1-6 are shown in Table 1 above. BC is the experimental blank control, NC is the experimental negative control, and PC is the positive control. As shown in the figure, the IL-6 content in the NC group was significantly higher than that in the blank control group (P < 0.05), indicating the validity of the experiment. As shown in the table, when the same safe concentration of the samples from the examples and comparative examples was added, Example 3 showed the best effect, with a downregulation rate of 82.68% for the IL-6 inflammatory factor induced by exogenous stimulation, close to the downregulation rate of the positive control PC, and significantly different from the negative control group (P < 0.05). Furthermore, the downregulation rate of cellular inflammatory factors in the examples was all above 70%, better than any of the comparative examples, indicating the rationality of the formulation of these eight plant compounds. Their combined effect is synergistic, and the plant compositions can effectively inhibit the release of the inflammatory factor IL-6, effectively improve skin inflammation, and indirectly improve acne and sebum release, demonstrating good oil control and acne-removing effects in this pathway.

[0154] Test 2: Inhibition of inflammatory factor IL-33

[0155] IL-33 (Interleukin-33) is an important immunomodulatory cytokine that plays a key role in allergic diseases (such as asthma and atopic dermatitis), autoimmune diseases (such as rheumatoid arthritis), and chronic inflammation. Therefore, this analysis primarily measured IL-33 inflammatory factors in the examples and comparative samples to assess their soothing effects and evaluate the pro-inflammatory role of IL-33 in suppressing excessive inflammation, such as in allergic diseases and chronic inflammation.

[0156] Cytotoxicity assay: RAW264.7 cell suspension was adjusted to different concentrations and added to 96-well cell plates. The cells were incubated overnight at 37°C with 5% CO2 for assay. Test samples were diluted with cell culture medium to different concentrations and added to cell plates, then incubated at 37°C with 5% CO2 for 48 hours. The culture medium was discarded, the cells were washed twice with PBS, and MTT working solution was added for further incubation. After incubation, the culture medium was discarded, DMSO was added, and the mixture was shaken and the absorbance was measured at 570 nm (with a background of 630 nm). Cytotoxicity was calculated based on the results, and, combined with cell morphology, the same concentration of samples showing no significant cytotoxicity was selected for further experiments.

[0157] RAW 264.7 cells were used to determine IL-33 levels. Cell suspensions were adjusted to an appropriate concentration and seeded into 96-well plates, incubated overnight at 37°C with 5% CO2. The experiment included a sample group (containing LPS and plant compositions from Examples 1-6 and Comparative Examples 1-6), a negative control (NC, containing LPS), a positive control (PC, containing tranilast and LPS), and a blank control (BC, without LPS). After 24 hours of treatment, the culture medium was slowly washed with PBS, and the cells were repeatedly freeze-thawed with added PBS. The supernatant was then collected, centrifuged, and the IL-33 level was detected using a double-antibody sandwich ELISA method: standards / samples, biotinylated detection antibody, HRP-labeled streptavidin, and TMB substrate were added sequentially. After color development, the absorbance at 450 nm was measured. The IL-33 inhibition rate was calculated using the formula: IL-33 inhibition rate = 1 - (sample OD - blank OD) / (NC - BC) * 100%. All experiments were performed in triplicate.

[0158] Table 2: Experimental results of the plant composition's inhibition of the inflammatory factor IL-33

[0159]

[0160] Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and 0.01 < P ≤ 0.05, denoted as "*"; 0.001 < P ≤ 0.01, denoted as "**"; 0.0001 < P ≤ 0.001, denoted as "***"; P ≤ 0.0001, denoted as "****".

[0161] The results of the experiments on the downregulation of the inflammatory factor IL-33 content by the plant compositions of Examples 1-6 and Comparative Examples 1-6 are shown in Table 2 above. Model validation showed that after repeated freeze-thaw stimulation with LPS, the intracellular IL-33 secretion level remained stable at 117.20 pg / ml, confirming the successful modeling. The positive control drug tranilast showed a significant inhibition rate of 92.45%, further verifying the sensitivity of the model to drug response. As shown in the table, when the same safe concentration of the sample from the examples and comparative examples was added, Example 2 showed the best effect, with an inhibition rate of 85.26% against the inflammatory factor IL-33, which was significantly different from the negative group (P < 0.05). Moreover, the inhibition rate of the inflammatory factor IL-33 in all examples was above 70%, which was better than any comparative example, further demonstrating the rationality of the eight plant combinations and their synergistic effect. It also shows that this plant composition can effectively inhibit the release of the inflammatory factor IL-33, effectively improve the pro-inflammatory effect of the skin, and indirectly improve skin acne and sebum release, thus having a good oil control and acne removal effect in this pathway.

[0162] Test 3: Cellular oil-controlling efficacy test

[0163] The compositions obtained in Examples 1-6 and Comparative Examples 1-6 were tested as follows:

[0164] Cytotoxicity assay: The test samples were diluted to different concentrations and added to SZ95 cell plates. The cytotoxicity was determined by the MTT assay. Combined with cell morphology, the same concentration of the sample with no obvious cytotoxicity was screened for the next step of the experiment.

[0165] Lipid synthesis inhibition assay: The concentration of SZ95 cell suspension was adjusted and added to 96-well cell plates. The cells were cultured overnight at 37°C with 5% CO2 for detection. The assay included a sample group (S), a negative control group (NC), a blank control group (BC), and a positive control group (PC). After sample addition, the cells were cultured for another 48 hours. After the assay, Nile Red staining reagent was added, and the fluorescence intensity was detected.

[0166] Result calculation and judgment:

[0167] Relative fat content = (S-BC) / (NC-BC)×100%.

[0168] Oil inhibition rate = [1 - (S - BC) / (NC - BC)] × 100%.

[0169] In the formula: S refers to the mean fluorescence intensity of the sample group; NC is the mean fluorescence intensity of the negative control group; BC is the mean fluorescence intensity of the blank control group.

[0170] Statistical and significance analysis was performed on the data, and the P-value was calculated. P < 0.05 indicates a significant difference. The sample group showed a positive oil inhibition rate and a significant difference compared to the negative control group (P < 0.05), thus the sample can be considered to have oil-controlling efficacy. (Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and 0.01 < P ≤ 0.05 is denoted as "*"; 0.001 < P ≤ 0.01 is denoted as "**"; 0.0001 < P ≤ 0.001 is denoted as "***"; P ≤ 0.0001 is denoted as "****").

[0171] Table 3: Experimental results of the composition's inhibitory effect on lipid synthesis

[0172]

[0173] The results of the experiments on the inhibition of sebum content in human sebaceous gland cells in Examples 1-6 and Comparative Examples 1-6 are shown in Table 3 above. BC is the experimental blank control, NC is the experimental negative control, and PC group is the positive control. As shown in the table, when the same safe concentration of the sample from the examples and comparative examples was added, Example 2 showed the highest inhibition rate of intracellular sebum synthesis, at 55.80%, which was higher than the positive control PC group and significantly different from the negative control group (P < 0.05), indicating its excellent sebum synthesis inhibition effect. Furthermore, all examples showed varying degrees of inhibition on sebum synthesis, with inhibition rates all above 30%. The inhibition rate of any example sample was better than that of any comparative example, further demonstrating the rationality of this formulation and its synergistic effect. This also indicates that this oil-controlling and acne-removing composition has a good inhibitory effect on cellular sebum secretion and can exert a good oil-controlling effect.

[0174] Test 4: Propionibacterium acnes antibacterial test

[0175] Propionibacterium acnes is a common parasitic bacterium in human hair follicles and sebaceous glands. The growth of Propionibacterium acnes is closely related to the occurrence of acne. When the secretions inside the hair follicles and sebaceous glands increase and are not discharged smoothly, a large amount of culture medium for Propionibacterium acnes can appear. Propionibacterium acnes will multiply in large numbers, which will lead to a series of acne symptoms. Inhibition of Propionibacterium acnes can, to a certain extent, indicate its potential acne-removing effect.

[0176] The in vitro antibacterial efficacy of Examples 1-6 and Comparative Examples 1-6 was investigated using a quantitative suspension antibacterial test: 24-hour fresh slant culture of the test bacteria was washed with PBS and diluted with PBS to approximately 5.0 × 10⁻⁶. 5 CFU / mL -4.5×10 6Prepare a CFU / mL bacterial suspension. Take a sterile test tube, add 5.0 mL of sample (use either stock solution or diluent as specified in the instructions), incubate in a 20℃±1℃ water bath for 5 min, then add 0.1 mL of the test bacterial suspension, mix quickly and start timing immediately. After the test bacteria have interacted with the sample for the time specified in the instructions, inoculate 1.0 mL of the test bacteria and sample mixture into two Petri dishes and pour the culture medium. If the bacterial count cannot be counted, perform a 10-fold serial dilution with PBS, select an appropriate dilution, inoculate 1.0 mL into two Petri dishes, and perform viable cell culture and counting. Simultaneously, use PBS instead of the sample to perform a parallel test as a positive control. The recovered colony count for the positive control should be 1.0 × 10⁻⁶. 4 CFU / mL - 9.0 × 10 4 The concentration of CFU / mL was between [value missing]. PBS and culture medium from the same batch were used as negative controls. All test and control samples were cultured at 36℃±1℃, and bacterial vegetative cells were cultured for 48 hours before the final results were observed. The experiment was repeated three times, and the inhibition rate was calculated.

[0177] Antibacterial rate calculation: X=[(A0-A1) / A0]×100%.

[0178] In the formula, X is the inhibition rate (%), A0 is the amount of bacteria recovered in the positive control group (CFU / mL), and A1 is the amount of bacteria recovered in the experimental group (CFU / mL).

[0179] Results determination: If the antibacterial rate is ≥50%-90%, it is determined to have an antibacterial effect; if the antibacterial rate is ≥90%, it is determined to have a strong antibacterial effect.

[0180] Table 4: Results of the experiment on the antibacterial rate against Propionibacterium acnes

[0181]

[0182] Table 4 shows that the plant composition samples of the same concentration prepared in Examples 1-6 and Comparative Examples 1-6 all exhibited inhibitory effects against Propionibacterium acnes, with all samples showing an inhibition rate of over 55%. Examples 2 and 3 showed the best results, with an inhibition rate exceeding 98%, and all examples showed an inhibition rate exceeding 80%. The antibacterial effect of each example was stronger than that of the comparative examples, indicating that the eight plants could work synergistically, further demonstrating the rationality of the formulation. This also indicates that the plant composition has a good inhibitory effect on Propionibacterium acnes, effectively reducing the growth of acne-related bacteria and providing good relief and improvement for acne, thus exhibiting good acne-removing efficacy.

[0183] In summary, this application uses eight plants as sources, references the theory of principal, assistant, adjuvant, and guide herbs in traditional Chinese medicine formulation, and selects suitable medicinal components based on a complete four-in-one system of "clearing the source, unblocking, treating the symptoms, and guiding the flow" in traditional Chinese medicine formulation. Through steps such as high-pressure microfluidic extraction, reduced-pressure concentration, high-speed centrifugation, membrane separation, concentration, and preparation, an oil-controlling and acne-removing plant composition is obtained. Based on network pharmacology analysis, a protein-protein interaction network was constructed, and core target enrichment analysis was performed, revealing a strong correlation with multiple pathways related to acne and sebum. Efficacy tests show that this plant composition can effectively inhibit sebum synthesis, inhibit the expression of inflammatory factors IL-6 and IL-33, and inhibit the growth of Propionibacterium acnes, effectively exerting good oil-controlling and acne-removing effects. Furthermore, the composition itself is natural, safe, and non-irritating. When added as an active ingredient to daily chemical products, it can effectively improve skin conditions such as acne, pimples, and increased sebum production, demonstrating excellent oil-controlling and acne-removing effects.

[0184] The embodiments described above are merely illustrative of several implementation methods of this application, intended to facilitate a detailed understanding of the technical solutions of this application, but should not be construed as limiting the scope of protection of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the scope of protection of this application. Furthermore, it should be understood that after reading the above teachings of this application, those skilled in the art can make various alterations or modifications to this application, and the equivalent forms obtained also fall within the scope of protection of this application. It should also be understood that technical solutions obtained by those skilled in the art based on the technical solutions provided in this application through logical analysis, reasoning, or limited experimentation are all within the scope of protection of the appended claims. Therefore, the scope of protection of this patent application should be determined by the content of the appended claims, and the specification can be used to interpret the content of the claims.

Claims

1. A plant-based composition with oil-controlling and acne-reducing effects, characterized in that, The composition comprises the following components in parts by weight: 1-50 parts of Rheum palmatum extract, 1-50 parts of Scutellaria baicalensis extract, 1-50 parts of Sophora flavescens extract, 1-50 parts of Carthamus tinctorius extract, 1-50 parts of Campsis grandiflora extract, 1-30 parts of Chrysanthemum extract, 1-30 parts of Celosia cristata extract, and 1-30 parts of Rosa rugosa extract.

2. The plant composition with oil-controlling and acne-removing effects according to claim 1, characterized in that, The rhubarb extract includes an aqueous or alcoholic extract of rhubarb; The Scutellaria baicalensis extract includes an aqueous extract or an alcoholic extract of Scutellaria baicalensis; and / or The Sophora flavescens extract includes an aqueous extract or an alcoholic extract of Sophora flavescens; and / or The safflower extract includes an aqueous or alcoholic extract of safflower; and / or The trumpet vine flower extract includes an aqueous extract or an alcoholic extract of the trumpet vine flower; and / or The chrysanthemum extract includes an aqueous or alcoholic extract of chrysanthemum; and / or The cockscomb flower extract includes an aqueous or alcoholic extract of cockscomb flower; and / or The rose extract includes rose water extract or rose alcohol extract.

3. A method for preparing the plant composition with oil-controlling and acne-removing effects according to any one of claims 1 to 2, characterized in that, include: A plant composition with oil-controlling and acne-reducing effects was prepared using the extracts of Rheum palmatum, Scutellaria baicalensis, Sophora flavescens, Carthamus tinctorius, Campsis grandiflora, Chrysanthemum morifolium, Celosia cristata, and Rosa rugosa.

4. The method for preparing the plant composition with oil-controlling and acne-removing effects according to claim 3, characterized in that, include: The following ingredients were crushed and mixed to prepare a preparatory material: Rheum palmatum, Scutellaria baicalensis, Sophora flavescens, Carthamus tinctorius, Campsis grandiflora, Chrysanthemum morifolium, Celosia cristata, and Rosa rugosa. The prepared material is subjected to high-pressure microfluidic extraction after adding an extraction solvent to prepare an extract. The extract is concentrated under reduced pressure to prepare a concentrate; the concentrate is mixed with a stabilizer and then reconstituted, centrifuged, and the supernatant is collected. as well as The supernatant was subjected to membrane filtration to prepare a plant composition with oil-controlling and acne-removing effects.

5. The method for preparing the plant composition with oil-controlling and acne-removing effects according to claim 4, characterized in that, The method satisfies one or more of the following conditions: (1) The extraction solvent includes one or more of water, ethanol, or an aqueous solution of ethanol; (2) The mass-to-volume ratio of the prepared material to the extraction solvent is 1:(10-30)g / mL; (3) The conditions for high-pressure microjets extraction include: pressure of 80 MPa - 120 MPa, extraction temperature of 20℃ - 60℃, and extraction time of 1h - 3h; (4) The conditions for vacuum concentration include: pressure of 2 kPa-20 kPa and temperature of 40℃-60℃; (5) The centrifugation speed is 5000 r / min - 20000 r / min; (6) The stabilizer includes one or more of butanediol, dipropylene glycol, glycerol, and 1,3-propanediol; and (7) The filter membranes used in membrane filtration include one or more of microfiltration membranes, ultrafiltration membranes and nanofiltration membranes; The microfiltration membrane has a pore size of 0.1 μm-3 μm, the ultrafiltration membrane has a pore size of 10 nm-50 nm, and the nanofiltration membrane has a pore size of 100 Da-1000 Da.

6. A product with oil-controlling and acne-reducing effects, characterized in that, This includes the plant composition with oil-controlling and acne-removing effects as described in any one of claims 1 to 2, or the plant composition with oil-controlling and acne-removing effects prepared by the preparation method described in any one of claims 3 to 5.

7. The product with oil-controlling and acne-removing effects according to claim 6, characterized in that, The product includes the plant composition and pharmaceutically acceptable excipients; The amount of the plant composition with oil-controlling and acne-removing effects added to the product is 0.1%-5% by mass.

8. The product with oil-controlling and acne-removing effects according to claim 7, characterized in that, The product includes the plant composition and cosmetic excipients.

9. The product with oil-controlling and acne-removing effects according to claim 8, characterized in that, Excipients acceptable in the cosmetics field include one or more of fillers, diluents, lubricants, wetting agents, and adhesives.

10. The product with oil-controlling and acne-removing effects according to any one of claims 6 to 9, characterized in that, The product with oil-controlling and acne-reducing effects may be in one or more of the following forms: cream, liquid, lotion, and foam; and / or The products with oil-controlling and acne-reducing effects include one or more of the following: toner, serum, cream, mask, and gel.

Images